Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 7

Integrity Analysis and Functional Sperm Assessments to Predict Boar Fertility Performance

Tainã F Cardoso1, Antonio Sergio Varela Jr2, Estela F Silva1, Mariana B Jorge2, Adalto Bianchini2, Carine D Corcini1,3*

1Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brasil
2Instituto de Ciências Biológicas, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brasil
3ReproPel, Faculdade de Veterinária, Campus Capão do Leão, Universidade Federal de Pelotas (UFPel), Pelotas, RS, Brasil

*Corresponding Author: Carine D Corcini, Programa de Pós-Graduação em Ciências Fisiológicas, Universidade Federal do Rio Grande (FURG), Rio Grande, RS, Brasil.

Received: January 17, 2022; Published: June 24, 2022

Abstract

Methods for estimating the in vitro fertility quality of a semen sample for use in artificial insemination have been investigated for a long time; however, for animals with high reproductive performance, these methods still have many shortcomings. Furthermore, there have been few reports on the peculiarities of swine sperm cells, especially regarding factors that influence fertility. Thus, some characteristics of sperm integrity and functionality may serve as indicators of reproductive performance in swine. The present study evaluated the morphophysiology of spermatozoa from swine with high and low in vivo farrowing rates. Animals with high fertility had higher mitochondrial functionality (p < 0.05), lower plasma membrane fluidity (p < 0.01) and higher total antioxidant capacity (p < 0.01). The last two parameters were positively correlated with in vivo fertility (r = 0.77, p = 0.0003; and r = 0.63, p = 0.0049; respectively). These findings indicate that certain parameters affect fertility and are potentially useful factors for evaluating semen and indicating the farrowing rate in swine.

Keywords: Plasma Membrane Fluidity; Sperm Physiology; Farrowing Rate

References

  1. Agarwal A., et al. “Proteomics, oxidative stress and male infertility”. Reproductive BioMedicine Online 29 (2014): 32-58.
  2. Akpa GN., et al. “Relationships between Body and Scrotal Measurements, and Semen characteristics in Yankasa ram”. Journal of Veterinary Science 1 (2012): 7-10.
  3. Alavi SMH., et al. “Spermatozoa motility in the Persian sturgeon, Acipenserpersicus: effects of pH, dilution rate, ions and osmolality”. Reproduction 128 (2004): 819-828.
  4. Amado LL., et al. “A method to measure total antioxidant capacity against peroxyl radicals in aquatic organisms: Application to evaluate microcystins toxicity”. Science of the Total Environment 407 (2009): 2115-2123.
  5. Antunes RC. “Avanço tecnológico e aplicabilidade da técnica de congelamento de sêmen suíno”. Revista Brasileira de Reprodução Animal 31 (2007): 60-63.
  6. Behan JR., et al. “A field investigation of intra-cervical insemination with reduced sperm numbers in gilts”. Theriogenology 66 (2006): 338-343.
  7. Benedetti S., et al. “Differences in blood and semen oxidative status in fertile and infertile men, and their relationship with sperm quality”. Reproductive BioMedicine Online 25 (2012): 300-306.
  8. Birck A., et al. “In vitro induction of the acrosome reaction in bull sperm and the relationship to field fertility using low-dose inseminations”. Theriogenology 73 (2010): 1180-1191.
  9. Broekhuijse MLWJ., et al. “Field data analysis of boar semen quality”. Reproduction in Domestic Animals 46 (2011): 59-63.
  10. Broekhuijse MLWJ., et al. “Relationship of flow cytometric sperm integrity assessments with boar fertility performance under optimized field conditions”. Journal of Animal Science 90 (2012): 4327-4336.
  11. Broekhuijse MLWJ., et al. “The value of microscopic semen motility assessment at collection for a commercial artificial insemination centre, a retrospective study on factors explaining variation in pig fertility”. Theriogenology 77 (2012a): 1466-1479.
  12. Broekhuijse MLWJ., et al. “Application of computer-assisted semen analysis to explain variations in pig fertility”. Journal of Animal Science 90 (2012b): 779-789.
  13. Colégio Brasileiro de Reprodução Animal. “Manual para exame andrológico e avaliação de sêmen animal”. 2nd edition. Belo Horizonte (1998).
  14. Corcini CD., et al. “In vitro assays for vesper mice (calomyslaucha) sperm using coincubation and oocyte’s cryopreservation”. Journal of Experimental Zoology 154 (2012): 96-102.
  15. Correa JR., et al. “Relationships among frozen-thawed sperm characteristics assessed via the rotine semen analysis, sperm functional tests and fertility of bulls in artificial insemination program”. Theriogenology 48 (1997): 721-731.
  16. De Ambrogi M., et al. “Viability and DNA fragmentation in differently sorted boar spermatozoa”. Theriogenology 66 (2006): 1994-2000.
  17. Domínguez-Rebolledo AE., et al. “Response of thawed epididymal red deer spermatozoa to increasing concentrations of hydrogen peroxide, and importance of individual male variability”. Reproduction in Domestic Animals 46 (2011): 393-403.
  18. Evenson DP., et al. “Flow cytometric evaluation of boar semen by the sperm chromatin structure assay as related to cryopreservation and fertility”. Theriogenology 41 (1994): 637-651.
  19. Fernández-Gago R., et al. “Seminal plasma applied post-thawing affects boar sperm physiology: A flow cytometry study”. Theriogenology 80 (2013): 400-410.
  20. Ferreira ALA and Matsubara LS. “Radicais livres: conceitos, doenças relacionadas, sistema de defesa e estressee oxidativo”. Revista da Associação Médica Brasileira 43 (1997): 1-16.
  21. Gadella BM., et al. “Capacitation and the acrosome reaction in equine sperm”. Animal Reproduction Science 68 (2001): 249-265.
  22. Gillan L., et al. “Flow cytometric evaluation of sperm parameters in relation to fertility potential”. Theriogenology 63 (2005): 445-457.
  23. Graham JK. “Assessment of sperm quality: a flow cytometric approach”. Animal Reproduction Science 68 (2001): 239-247.
  24. Hu JH., et al. “Effect of GynostemmaPentaphyllum Polysaccharide on boar spermatozoa quality following freezing-thawing”. Cryobiology 59 (2009): 244-249.
  25. Hurtado LA., et al. “AMP-activated kinase, AMPK, is involved in the maintenance of plasma membrane organization in boar spermatozoa”. Biochimica et Biophysica Acta 1828 (2013): 2143-2151.
  26. Jimenez T., et al. “Na,K-ATPase alpha4 isoform is essential for sperm fertility”. Proceedings of the National Academy of Sciences of the United States of America 11 (2010): 644-649.
  27. Kroemer G., et al. “Mitochondrial control of apoptosis”. Immunology Today 18 (1997): 44-51.
  28. Lanzafame FM., et al. “Oxidative stress and medical antioxidant treatment in male infertility”. Reproductive BioMedicine Online 19 (2009): 638-659.
  29. Levine RL., et al. “Determination of carbonyl content in oxidatively modified proteins”. Methods Enzymology 186 (1990): 464-478.
  30. Lopes TM., et al. “Effect of copper onion content in isolated mantle cells of the marine clam Mesodesmamactroides”. Environmental Toxicology and Chemistry 30 (2011): 1582-1585.
  31. Ly JD., et al. “The mitochondrial membrane potential (deltapsi (m)) in apoptosis; an update”. Apoptosis 8 (2003): 115-128.
  32. Martínez-López P., et al. “Mouse sperm K+ currents stimulated by pH and cAMP possibly coded by Slo3 channels”. Biochemical and Biophysical Research Communications 381 (2009): 204-209.
  33. Melo MIV., et al. “Teste hiposmótico para avaliação da viabilidade do sêmen equino resfriado com diferentes diluidores”. Arquivo brasileiro de medicina veterinária e zootecnia 57 (2005): 757-763.
  34. Ménézo Y., et al. “Stress oxydantetfertilité: faussesévidences et mauvaisesrecettes”. Gynécologie Obstétrique Fertilité 4 (2012): 787-796.
  35. Nordberg J and Arnér ESJ. “Reactive oxygen species, antioxidants and the mammalian thioredoxin system”. Free Radical Biology and Medicine 31 (2001): 1287-1312.
  36. Novak S., et al. “Biomarkers of in vivo fertility in sperm and seminal plasma of fertile stallions”. Theriogenology 7 (2010): 956-967.
  37. Oakes KD., et al. “Utility of the TBARS assay in detecting oxidative stress in white sucker (Catostomuscommersoni) populations exposed to pulp mill effluent”. Aquatic Toxicology 63 (2003): 447-463.
  38. Petrunkina AM., et al. “Functional significance of responsiveness to capacitating conditions in boar spermatozoa”. Theriogenology 64 (2005): 1766-1782.
  39. Piehler E., et al. “Dynamic quantification of the tyrosine phosphorylation of the sperm surface proteins during capacitation in vitro”. Cytom 69 (2005): 1062-1070.
  40. Pursel VG and Johnson LA. “Freezing of boar spermatozoa: Freezing capacity with concentrated semen and a new thawing procedure”. Journal of Animal Science 40 (1975): 99-102.
  41. Quinn JP., et al. “Studies of the distribution of the major cations in semen and male accessory secretions”. Journal of Reproduction and Infertility 10 (1965): 379-388.
  42. Rajender S., et al. “Mitochondria, spermatogenesis and male infertility”. Mitochondrion 10 (2010): 419-428.
  43. Rodriguez-Martinez H and Larsson B. “Assessment of sperm fertilizing ability in farm animal”. Acta Agriculturae Scandinavica B 29 (1998): 12-18.
  44. Senger PL. “Pathways to pregnancy and parturition. 2nd edition”. Pullman (WA): Current Conceptions (2003): 271.
  45. Songsasen N and Leibo SP. “Cryopreservation of mouse spermatozoa: Effect of seeding on fertilizing ability of cryopreserved spermatozoa”. Cryobiology 35 (1997): 240-254.
  46. Valsa J., et al. “Split ejaculation study: semen parameters and calcium and magnesium in seminal plasma”. Central European Journal of Urology 65 (2012): 216-218.
  47. Watson PF. “The causes of reduced fertility with cryopreserved semen”. Animal Reproduction Science 61 (2000): 481-492.

Citation

Citation: Carine D Corcini., et al. “Integrity Analysis and Functional Sperm Assessments to Predict Boar Fertility Performance".Acta Scientific Veterinary Sciences 4.7 (2022): 119-127.

Copyright

Copyright: © 2022 Carine D Corcini., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is May 20, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US