Use of Hemostatic Compression in the Making of the Nx 5/6 Experimental Model in Wistar Rats
Fellipe Ferreira Lemos de Medeiros1, Natasha Nogueira Ferreira2,
Cecília Ribeiro Castañon2, Patricia Pereira Almeida3, Ágatha Cristie
Menezes3, D'Angelo Carlo Magliano4, Milena Barcza Stockler-Pinto4,
Maria de Lourdes Gonçalves Ferreira5, Viviane Alexandre Nunes
Degani6* and Nayro Xavier de Alencar5
1Postgraduate Program in Clinic and Animal Breeding, Universidade Federal
Fluminense, Niterói, RJ, Brazil
2Autonomus Veterinarian, Niterói-RJ, Brazil
3Postgraduate Cardiovascular Sciences Post Graduate Program, Fluminense Federal
University (UFF), Niterói-RJ, Brazil
4Cardiovascular Sciences Post Graduate Program, Fluminense Federal University (UFF),
5Clinic and Animal Reproduction Post Graduate Program, Fluminense Federal
University (UFF), Niterói-RJ, Brazil
6Morphology Department, Biomedical Institute, Fluminense Federal University (UFF),
*Corresponding Author: Viviane Alexandre Nunes Degani, Morphology
Department, Biomedical Institute, Fluminense Federal University (UFF),
March 01, 2022; Published: March 21, 2022
Objectives: To enhance the technique for the creation of the experimental model for chronic kidney disease with 5/6 nephrectomy
and to establish parameters for the postoperative evaluation of animals undergoing the surgical procedure.
Methods: Twenty-one male Wistar rats aged over two months were divided into two groups, namely, SHAM (n = 9) and 5/6 Nx (n
= 12). The animals were anesthetized intraperitoneally with ketamine, midazolam and tramadol. In the 5/6 Nx group, bipolar nephrectomy
of one kidney and total nephrectomy of the contralateral kidney were performed in a single surgical procedure, whereas
the SHAM group underwent laparotomy without nephrectomy. The following measurements were carried out in both groups: 24-h
urinary volume and proteinuria; total body weight; systolic blood pressure; and serum urea and creatinine.
Conclusion: The modifications in the technique proved to be feasible, providing longer survival of the animals in the medium term,
fewer intraoperative complications and effectiveness for the construction of the experimental model.
Keywords: Nephrectomy; Kidney Disease; Rodents
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