Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 4

Use of Hemostatic Compression in the Making of the Nx 5/6 Experimental Model in Wistar Rats

Fellipe Ferreira Lemos de Medeiros1, Natasha Nogueira Ferreira2, Cecília Ribeiro Castañon2, Patricia Pereira Almeida3, Ágatha Cristie Menezes3, D'Angelo Carlo Magliano4, Milena Barcza Stockler-Pinto4, Maria de Lourdes Gonçalves Ferreira5, Viviane Alexandre Nunes Degani6* and Nayro Xavier de Alencar5

1Postgraduate Program in Clinic and Animal Breeding, Universidade Federal Fluminense, Niterói, RJ, Brazil
2Autonomus Veterinarian, Niterói-RJ, Brazil
3Postgraduate Cardiovascular Sciences Post Graduate Program, Fluminense Federal University (UFF), Niterói-RJ, Brazil
4Cardiovascular Sciences Post Graduate Program, Fluminense Federal University (UFF), Niterói-RJ, Brazil

5Clinic and Animal Reproduction Post Graduate Program, Fluminense Federal University (UFF), Niterói-RJ, Brazil
6Morphology Department, Biomedical Institute, Fluminense Federal University (UFF), Niterói-RJ, Brazil

*Corresponding Author: Viviane Alexandre Nunes Degani, Morphology Department, Biomedical Institute, Fluminense Federal University (UFF), Niterói-RJ, Brazil.

Received: March 01, 2022; Published: March 21, 2022

Abstract

Objectives: To enhance the technique for the creation of the experimental model for chronic kidney disease with 5/6 nephrectomy and to establish parameters for the postoperative evaluation of animals undergoing the surgical procedure.

Methods: Twenty-one male Wistar rats aged over two months were divided into two groups, namely, SHAM (n = 9) and 5/6 Nx (n = 12). The animals were anesthetized intraperitoneally with ketamine, midazolam and tramadol. In the 5/6 Nx group, bipolar nephrectomy of one kidney and total nephrectomy of the contralateral kidney were performed in a single surgical procedure, whereas the SHAM group underwent laparotomy without nephrectomy. The following measurements were carried out in both groups: 24-h urinary volume and proteinuria; total body weight; systolic blood pressure; and serum urea and creatinine.

Conclusion: The modifications in the technique proved to be feasible, providing longer survival of the animals in the medium term, fewer intraoperative complications and effectiveness for the construction of the experimental model.

Keywords: Nephrectomy; Kidney Disease; Rodents

References

  1. National Kidney Foundation. “K/DOQI clinical practice guidelines for chronic kidney disease: evaluation, classification, and stratification”. American Journal of Kidney Diseases: The Official Journal of the National Kidney Foundation2 (2002): S1-266.
  2. Bargman JM and Skorecki K. “Chronic kidney disease”. In: Longo D et al. (Eds.). Harrison’s Principles of Internal Medicine. 18. ed. New York: McGraw-Hill Education (2011): 2308-2321.
  3. Jha V., et al. “Chronic kidney disease: Global dimension and perspectives”. The Lancet9888 (2013): 260-272.
  4. Lv JC and Zhang LX. “Prevalence and Disease Burden of Chronic Kidney Disease”. Advances in Experimental Medicine and Biology 1165 (2019): 3-15.
  5. Câmara NOS., et al. “Kidney disease and obesity: Epidemiology, mechanisms and treatment”. Nature Reviews Nephrology 3 (2017): 181-190.
  6. Bao YW., et al. “Kidney disease models: tools to identify mechanisms and potential therapeutic targets”. Zoological Research2 (2018): 72-86.
  7. Wei Q and Dong Z. “Mouse model of ischemic acute kidney injury: Technical notes and tricks”. American Journal of Physiology - Renal Physiology11(2012): 54-76.
  8. Pearce RM. “The influence of the reduction of kidney substance upon nitrogenous metabolism”. The Journal of Experimental Medicine5 (1908): 632-644.
  9. Bradford JR. “The results following partial Nephrectomy and the influence of the Kidney on Metabolism”. The Journal of Physiology6 (1899): 415-496.
  10. Macnider WD. “The development of the chronic nephritis induced in the dog by uranium nitrate. A functional and pathological study with observations on the formation of urine by the altered kidneys”. Journal of Experimental Medicine3 (1929): 387-410.
  11. Heymann W., et al. “Production of nephrotic syndrome in rats by Freund’s adjuvants and rat kidney suspensions”. Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine (New York, N.Y.)4 (1959):660-664.
  12. Allison ME., et al. “Pathophysiology of experimental glomerulonephritis in rats”. Journal of Clinical Investigation5 (1974):1402-1423.
  13. Kumano K., et al. “A new method of inducing experimental chronic renal failure by cryosurgery”. Kidney International3 (1986): 433-436.
  14. He J., et al. “Bone marrow stem cells-derived microvesicles protect against renal injury in the mouse remnant kidney model”. Nephrology5 (2012): 493-500.
  15. Chanutin A and Ferris JR. “Experimental renal insufficiency produced by partial nephrectomy: I. control diet”. Archives of Internal Medicine5 (1932):767-787.
  16. Platt R., et al. “Experimental renal failure”. Clinical Science3 (1952): 217-231.
  17. Avioli LV., et al. “Intestinal Calcium Absorption: Nature of Defect in Chronic Renal Disease”. Science3909 (1969) :1154 LP - 1156.
  18. Ergür BU., et al. “The effects of α-lipoic acid on aortic injury and hypertension in the rat remnant kidney (5/6 nephrectomy) model”. Anatolian Journal of Cardiology 6 (2015): 443-449.
  19. Hamzaoui M., et al. “5/6 nephrectomy induces different renal, cardiac and vascular consequences in 129/Sv and C57BL/6JRj mice”. Scientific Reports1 (2020):1-9.
  20. Boudet J., et al. “Experimental chronic renal failure in the rat by electrocoagulation of the renal cortex”. Kidney International1 (1978): 82-86.
  21. Yin J., et al. “Renalase attenuates hypertension, renal injury and cardiac remodelling in rats with subtotal nephrectomy”. Journal of Cellular and Molecular Medicine6 (2016):1106-1117.
  22. Gava AL., et al. “Effects of 5/6 nephrectomy on renal function and blood pressure in mice”. International Journal of Physiology, Pathophysiology and Pharmacology3 (2012):167-173.
  23. Gabrielli MA., et al. “Avaliação histológica de agentes hemostáticos implantados em mandíbulas de coelhos Histologic Evaluation of Hemostatic Agents in the Rabbit Mandible”. Revista de Cirurgia e Traumatologia Buco-Maxilo-Facial2 (2009): 97-106.
  24. Teles F., et al. “Brazilian red propolis attenuates hypertension and renal damage in 5/6 renal ablation model”. PLoS ONE1 (2015): 1-15.
  25. Koppe L., et al. “Kidney cachexia or protein-energy wasting in chronic kidney disease: facts and numbers”. Journal of Cachexia, Sarcopenia and Muscle3 (2019): 479-484.
  26. Kosaka S., et al. “Effects of angiotensin II AT1-receptor blockade on high fat diet-induced vascular oxidative stress and endothelial dysfunction in dahl salt-sensitive rats”. Journal of Pharmacological Sciences2 (2013): 95-102.
  27. Bartlett PC., et al. “Case-control study of risk factors associated with feline and canine chronic kidney disease”. Veterinary Medicine International10 (2010): 34-46.
  28. Huidobro EJP., et al. “Estimation of glomerular filtration rate with creatinine”. Revista Medica de Chile3 (2018): 344-350.

Citation

Citation: Fellipe Ferreira Lemos de Medeiros., et al. “Use of Hemostatic Compression in the Making of the Nx 5/6 Experimental Model in Wistar Rats". Acta Scientific Veterinary Sciences 4.4 (2022): 88-94.

Copyright

Copyright: © 2022 Fellipe Ferreira Lemos de Medeiros., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US