Fellipe Ferreira Lemos de Medeiros¹, Natasha Nogueira Ferreira², Cecília Ribeiro Castañon², Patricia Pereira Almeida³, Ágatha Cristie Menezes³, D'Angelo Carlo Magliano⁴, Milena Barcza Stockler-Pinto⁴, Maria de Lourdes Gonçalves Ferreira⁵, Viviane Alexandre Nunes Degani⁶* and Nayro Xavier de Alencar⁵

¹Postgraduate Program in Clinic and Animal Breeding, Universidade Federal Fluminense, Niterói, RJ, Brazil
²Autonomus Veterinarian, Niterói-RJ, Brazil
³Postgraduate Cardiovascular Sciences Post Graduate Program, Fluminense Federal University (UFF), Niterói-RJ, Brazil
⁴Cardiovascular Sciences Post Graduate Program, Fluminense Federal University (UFF), Niterói-RJ, Brazil

⁵Clinic and Animal Reproduction Post Graduate Program, Fluminense Federal University (UFF), Niterói-RJ, Brazil
⁶Morphology Department, Biomedical Institute, Fluminense Federal University (UFF), Niterói-RJ, Brazil

*Corresponding Author: Viviane Alexandre Nunes Degani, Morphology Department, Biomedical Institute, Fluminense Federal University (UFF), Niterói-RJ, Brazil.

Received: March 01, 2022; Published: March 21, 2022

Abstract

Objectives: To enhance the technique for the creation of the experimental model for chronic kidney disease with 5/6 nephrectomy and to establish parameters for the postoperative evaluation of animals undergoing the surgical procedure.

Methods: Twenty-one male Wistar rats aged over two months were divided into two groups, namely, SHAM (n = 9) and 5/6 Nx (n = 12). The animals were anesthetized intraperitoneally with ketamine, midazolam and tramadol. In the 5/6 Nx group, bipolar nephrectomy of one kidney and total nephrectomy of the contralateral kidney were performed in a single surgical procedure, whereas the SHAM group underwent laparotomy without nephrectomy. The following measurements were carried out in both groups: 24-h urinary volume and proteinuria; total body weight; systolic blood pressure; and serum urea and creatinine.

Conclusion: The modifications in the technique proved to be feasible, providing longer survival of the animals in the medium term, fewer intraoperative complications and effectiveness for the construction of the experimental model.

Keywords: Nephrectomy; Kidney Disease; Rodents

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Citation

Citation: Fellipe Ferreira Lemos de Medeiros., et al. “Use of Hemostatic Compression in the Making of the Nx 5/6 Experimental Model in Wistar Rats". Acta Scientific Veterinary Sciences 4.4 (2022): 88-94.

Copyright

Copyright: © 2022 Fellipe Ferreira Lemos de Medeiros., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.