Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 4

Quantification of Gallic Acid, Rutin and Quercetin in Hydro-Ethanolic Extract of Holarrhena Antidysenterica using High Performance Thin Layer Chromatography (HPTLC)

Swapnil S Mehesare, Sunil P Waghmare*, Milind G Thorat, Sunil W Hajare, Ravindrakumar I Hatzade and Mahesh V Ingawale

Department of TVCC, Post Graduate Institute of Veterinary and Animal Science, Akola, MASFU, Nagpur, India

*Corresponding Author: Sunil P Waghmare, Department of TVCC, Post Graduate Institute of Veterinary and Animal Science, Akola, MASFU, Nagpur, India.

Received: February 14, 2022; Published: March 11, 2022

Abstract

Holarrhena antidysenterica possesses antidiarrhoeal and antibacterial activity. The present study was focused to detect bioactive compounds by high-performance liquid chromatography (HPLC). HPLC is a tool to separate mixture of compounds in analytical chemistry (identify, quantify, purify) but cannot be used to establish biological activity. HPLC was performed to separate mixture of compounds with varying molecular sizes like gallic acid, quercetin and rutin in hydro-ethanolic extracts of seeds of Holarrhena antidysenterica. These markers were detected, quantified and compared with standards at 200 - 450 nm wavelength using mobile phase Toulene: Ethyl Acetate: Formic Acid: Methanol (3:3:0.3:0.2) with CAMAG Scanner III system. The hydro-ethanolic seeds extracts of Holarrhena antidysenterica showed peak Rf values which were coinciding with standard Rf value of gallic acid, quercetin and rutin. Comparable Rf values were found for gallic acid, quercetin and rutin in sample and reference standard. This study confirmed the presence of gallic acid, quercetin and rutin in Holarrhena antidysenterica seeds hydro-ethanolic extract might be important for preventing diarrhoea. In conclusion, the hydro-ethanolic extract of seeds of Holarrhena antidysenterica could be a potential source of antidiarrhoeal, antibacterial, astringent and anti-inflammatory drugs.

Keywords: Gallic Acid; Holarrhena Antidysenterica; Hptlc; Rutin; Quercetin

References

  1. Mehesare SS. “Phytopharmacological and therapeutic study of polyherbal antidiarrhoeal formulations in goats”. Ph.D. Thesis, Maharashtra Animal and Fishery Sciences University, Nagpur, India (2018).
  2. Lather A., et al. “Pharmacological Potential of Ayurvedic Formulation: Kutajghan Vati - A Review”. Journal of Advanced Scientific Research 2 (2010): 41-45.
  3. Sharma DK., et al. “Evaluation of antidiarrhoeal activity of ethanolic extract of Holarrhena antidysenterica seeds in rats”. Veterinary World 12 (2015): 1392-1395.
  4. Singh M., et al. “Evaluation of Antibacterial and Therapeutic Potential of Holarrhena Antidysenterica Bark Extract in Coli Calf Diarrhea”. International Journal of Advanced Research 4.8 (2016): 1334-1339.
  5. Sinha S., et al. “Evaluation of phytochemical and pharmacological aspects of Holarrhena antidysenterica (Wall.): A comprehensive review”. Journal of Pharmacy Research 4 (2013): 88-92.
  6. Alam P., et al. “Stability-indicating densitometric high-performance thin layer chromatographic method for the quantitative analysis of biomarker naringin in the leaves and stems of Rumex vesicarius L”. Journal of Planar Chromatography 27 (2014): 204-209.
  7. Ravishankar MN., et al. “Chromatography” 744 (2000): 257-262.
  8. Sethi PD. “HPTLC, CBS Publishers and distributors 1st edition”. New Delhi” (1996): 39.
  9. Sajeeth CI., et al. “Quantitative estimation of gallic Acid, rutin and quercetin in certain herbal plants by HPTLC method”. Pelagia Research Library Der Chemica Sinica2 (2010): 80-85.
  10. Shrestha SP., et al. “Nitric Oxide Production Inhibitory Activity of Flavonoids Contained in Trunk Exudates of Dalbergia sissoo”. Journal of Natural Products.1 (2008): 98-101.
  11. Brijesh SP., et al. “Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: Validating its traditional usage, BMC Complement Altern”. Medicine 9 (2009): 1-12.
  12. Nahak G and RK Sahu. “Free Radical Scavenging activity of Multivitamin Plant (Sauropusandrogynus Merr)”. Researcher 2.11 (2014): 6-14.
  13. Ballal M., et al. “Antibacterial activity of Holarrhena antidysenterica [kurchi] against the enteric pathogens”. Indian Journal of Pharmacology (2001): 392-393.
  14. Ganapathy PS., et al. “Pharmacognostic and phytochemical evaluation of Holarrhena antidysenterica wall”. The Asian and Australasian Journal of Plant Science and Biotechnology 1 (2009): 47-50.
  15. Hajare SW., et al. “Anti-inflammatory activity of the 90% ethanolic extract of Dalbergia sissoo Leaves”. Fitoterapia2 (2001): 131-139.
  16. Gayathri P., et al. “Screening and Quantitation of Phytochemicals and Nutritional Components of the Fruit and Bark of Helicteres Isora”. Hygeia Journal for Drugs and Medicines 1 (2010): 57-62.

Citation

Citation: Sunil P Waghmare., et al. “Quantification of Gallic Acid, Rutin and Quercetin in Hydro-Ethanolic Extract of Holarrhena Antidysenterica using High Performance Thin Layer Chromatography (HPTLC) ". Acta Scientific Veterinary Sciences 4.4 (2022): 43-49.

Copyright

Copyright: © 2022 Sunil P Waghmare., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is October 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US