Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 4

Chromatographic Analysis of Vitamin E (α - Tocopherol) in Blood of Chicken Under Heat Stress and its Correlation with Serum Muscle Injury Markers

Abhishek Sharma, Madhu Swamy*, Kshemankar Shrman, Yamini Verma and Amita Dubey

Department of Veterinary Pathology, Nanaji Deshmukh Veterinary Sciences University, Jabalpur, India

*Corresponding Author: Madhu Swamy, Department of Veterinary Pathology, Nanaji Deshmukh Veterinary Sciences University, Jabalpur, India.

Received: February 28, 2022; Published: March 08, 2022


Study was carried out to determine the effect of heat stress on broiler birds during hot humid climate with mean ambient temperature of 42 0C and a 58 per cent relative humidity on the serum muscle injury markers and vitamin E. For the study broiler birds from different poultry outlets covering a population of 1.5 lakh birds were selected. Samples were also collected from 25 apparently healthy birds with no indication of heat stress. Approximately 2-4ml of blood was collected in vacutainers without anticoagulant at the time of slaughter. The two muscle injury markers, creatinine kinase (CK) and lactate dehydrogenase (LDH) were found to be significantly raised in the serum of birds under heat stress (p ≤ 0.01). Concentration of vitamin E in the form of α tocopherol was estimated by Ultra High-Performance Liquid Chromatography (UHPLC). A strong linear correlation was determined between occurrence of heat stress and serum vitamin E concentration at the 0.01 level (2-tailed). This study indicates the vitamin E requirement of birds during hot humid climate is more and supplementation of Vitamin E in diet may prevent losses due to heat stress.

Keywords: Chromatography; Heat Stress; Creatinine Kinase; Lactate Dehydrogenase and Vitamin E.


  1. Ajakaiye JJ., et al. “Effects of heat stress on some blood parameters and egg production of Shika Brown layer chickens transported by road”. Biological Research43.2 (2010): 183-189.
  2. Sahin K., et al. “Role of dietary zinc in heat-stressed poultry: A review”. Poultry Science88 (2009): 2176-2183.
  3. Khan R., et al. “Effect of vitamin E in heat-stressed poultry”. World's Poultry Science Journal (2011): 469478.
  4. Dalolio FS., et al. “Heat stress andvitamin E in diets for broilers as a mitigating measure”. ActaScientiarum Animal Sciences 37 (2015): 419-427.
  5. Mujahid A., et al. “Mitochondrial oxidative damage in chicken skeletal muscle induced by acute heat stress”. The Journal of Poultry Science 4 (2007): 439-445.
  6. Xie J., et al. “Effects of acute and chronic heat stress on plasma metabolites, hormones and oxidant status in restrictedly fed broiler breeders”. Poultry Science7 (2015): 1635-1644.
  7. Kim YJ., et al. “Effects of dietary α-tocopherol, selenium and their different combinations on growth performance and meat quality of broiler chickens”. Poultry Science3 (2010): 603-608.
  8. Guetchom B., et al. “Effect of extra dietary vitamin E on preventing nutritional myopathy in broiler chickens”. Journal of Applied Poultry Research21 (2012): 548-555.
  9. Pompeu MA., et al. “Effect of vitamin E supplementation on growth performance, meat quality and immune response of male broiler chickens: a meta-analysis”. Livestock Science208 (2018): 5-13.
  10. Abidin SZ., et al. “Determination of vitamin E isomers in plasma using ultra performance liquid chromatography”. The Malaysian Journal of Analytical Sciences16 (2012): 71-81.
  11. Gezelinska Z., et al. “Plasma concentration of vitamin E, vitamin A and β - carotene in healthy men”. Polish Journal of EnvironmentalStudies16 (2007): 209-213.
  12. Khan RU., et al. “Effect of ascorbic acid in heat- stressed poultry”. World's Poultry Science Journal68 (2012): 477-490.
  13. Ozaki M., et al. “The in vivo cytoprotection of ascorbic acid against ischemia/reoxygenation injury of rat liver”. Archives of Biochemistry and Biophysics318.2 (1995): 439-445.
  14. Mitchell MA and Sandercock DA. “Creatine kinase isoenzyme profiles in the plasma of the domestic fowl (Gallus domesticus): effects of acute heat stress”. Research in Veterinary Science59 (1995): 30-34.
  15. Mikami T., et al. “Endurance exercise training inhibits activity of plasma GOT and liver caspase-3 of rats exposed to stress by induction of heat shock protein 70”. Journal of Applied Physiology964 (2004): 1776-1781.
  16. Sandercock DA., et al. “Thermoregulatory capacity and muscle membrane integrity are compromised in broilers compared with layers at the same age or body weight”. British Poultry Science3 (2006): 322-329.
  17. Huang Shu-cheng., et al. “Acute heat stress in broiler chickens and its impact on serum biochemical and electrolyte parameters”. Indian Journal of Animal Research 52 (2018): 683-686.
  18. Luo J., et al. “Effect of epigallocatechin gallate on growth performance and serum biochemical metabolites in heat-stressed broilers”. Poultry Science2 (2018): 599-606.
  19. da Silva ICM., et al. “Effect of vitamin E levels on the cell-mediated immunity of broilers vaccinated against coccidiosis”. Brazilian Journal of Poultry Science 13 (2011): 53-56.
  20. Bou R., et al. “Effect of dietary fish oil, α-tocopheryl acetate and zinc supplementation on the composition and consumer acceptability of chicken meat”. Poultry Science83.2 (2004): 282-292.
  21. Souza MGD., et al. “Use of vitamins C and E on ration for broilers kept in high temperature environment”. RevistaBrasileira de Zootecnia 40 (2011): 2192-2198.
  22. Singh M., et al. “Influence of dietary supplementation of Vitamin E in ameliorating adverse effects of ochratoxin on biochemical profile and immune response in broiler chickens”. Indian journal of Animal Sciences 82 (2016): 1447-145.
  23. Khattak FM., et al. “Comparative efficacy of different supplements used to reduce heat stress in broilers”. Pakistan Journal of Zoology44 (2012): 34-41.
  24. Sahin K and Kucuk O. “Effects of vitamin C and vitamin E on performance, digestion of nutrients carcass characteristics of Japanese quails reared under chronic heat stress”. Journal of Animal Physiology and Animal Nutrition85 (2001a): 335-341.
  25. Sahin K and Kucuk O. “Effects of vitamin E and selenium on performance, digestibility of nutrients, and carcass characteristics of Japanese quails reared under heat stress”. Journal of Animal Physiology and Animal Nutrition85 (2001b): 342-348.


Citation: Madhu Swamy., et al. “Chromatographic Analysis of Vitamin E (α - Tocopherol) in Blood of Chicken Under Heat Stress and its Correlation with Serum Muscle Injury Markers”. Acta Scientific Veterinary Sciences 4.4 (2022): 22-26.


Copyright: © 2022 Madhu Swamy., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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