Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 4

Chromatographic Analysis of Vitamin E (α - Tocopherol) in Blood of Chicken Under Heat Stress and its Correlation with Serum Muscle Injury Markers

Abhishek Sharma, Madhu Swamy*, Kshemankar Shrman, Yamini Verma and Amita Dubey

Department of Veterinary Pathology, Nanaji Deshmukh Veterinary Sciences University, Jabalpur, India

*Corresponding Author: Madhu Swamy, Department of Veterinary Pathology, Nanaji Deshmukh Veterinary Sciences University, Jabalpur, India.

Received: February 28, 2022; Published: March 08, 2022

Abstract

Study was carried out to determine the effect of heat stress on broiler birds during hot humid climate with mean ambient temperature of 42 0C and a 58 per cent relative humidity on the serum muscle injury markers and vitamin E. For the study broiler birds from different poultry outlets covering a population of 1.5 lakh birds were selected. Samples were also collected from 25 apparently healthy birds with no indication of heat stress. Approximately 2-4ml of blood was collected in vacutainers without anticoagulant at the time of slaughter. The two muscle injury markers, creatinine kinase (CK) and lactate dehydrogenase (LDH) were found to be significantly raised in the serum of birds under heat stress (p ≤ 0.01). Concentration of vitamin E in the form of α tocopherol was estimated by Ultra High-Performance Liquid Chromatography (UHPLC). A strong linear correlation was determined between occurrence of heat stress and serum vitamin E concentration at the 0.01 level (2-tailed). This study indicates the vitamin E requirement of birds during hot humid climate is more and supplementation of Vitamin E in diet may prevent losses due to heat stress.

Keywords: Chromatography; Heat Stress; Creatinine Kinase; Lactate Dehydrogenase and Vitamin E.

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Citation

Citation: Madhu Swamy., et al. “Chromatographic Analysis of Vitamin E (α - Tocopherol) in Blood of Chicken Under Heat Stress and its Correlation with Serum Muscle Injury Markers”. Acta Scientific Veterinary Sciences 4.4 (2022): 22-26.

Copyright

Copyright: © 2022 Madhu Swamy., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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Impact Factor1.008

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