Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 2

Use of Primer Design to Detect the Glycoprotein C Gene of Canine Herpes Virus by Polymerase Chain Reaction

Marco Vargas, María Antonieta Jara and Carlos Navarro*

Department of Medicina Preventiva Animal, Universidad de Chile, Chile

*Corresponding Author:Carlos Navarro, Department of Medicina Preventiva Animal, Universidad de Chile, Chile.

Received: February 04, 2022; Published: February 21, 2022

Abstract

The fundamental difference between a virus and microorganisms lies in the way they generate progeny and some viruses destroy the cell membrane, a phenomenon called cytolysis. The herpes virus has this cytolytic capacity and in veterinary medicine, several important herpes viruses have been described, among which is the Canine Herpes Virus type 1 (CaHV-1) and this study contributed both to the genomic characterization of a national isolate of the CaHV-1 virus -called RP5- as well as to small animal veterinary medicine with a specific, sensitive, and fast diagnostic method result. For this, detection of the CaHV-1 glycoprotein C gene was performed by Polymerase Chain Reaction using a pair of primers designed in silico. Using a temperature gradient thermocycler, the alignment temperature (55ºC) was established by the clear and unequivocal observation of a DNA fragment of approximately 200 bp. These fragments were sequenced and a percentage of nucleotide identity (NIP > 94%) was established with respect to the official GenBank data and indicates that the amplified fragment corresponds to the gC gene fragment of CaHV-1. Thus, this methodology constitutes another technique of choice for the detection of CaHV-1 in Small Animal Medicine.

Keywords: Canine Herpes Virus; Glycoprotein C; Diagnosis; In Silico

References

  1. “International Committee on Taxonomy of Viruses”. Virus Taxonomy (2020).
  2. Carter GR., et al. Herpesviridae. In: “A Concise Review of Veterinary Virology” G.R. Carter, D.J. Wise and E.F. Flores (Eds.) Publisher: International Veterinary Information Service. Ithaca, New York, USA (2006).
  3. Murphy FA., et al. “Veterinary virology. 3rd Ed. Academic Press. London, Inglaterra (1999).
  4. Papageorgiou KV., et al. “Genome Sequence of Canine Herpesvirus”. PLoS ONE5 (2016): e0156015.
  5. Madigan MT., et al. “ Biología de los microorganismos”. 10ª ed. Ed. Pearson. Madrid, España (2003): 1011.
  6. Liu F and Zhou ZH. “Comparative virion structures of human herpesviruses. In: Arvin A, Campadelli-Fiume G, Mocarski E, et al., editors. Human Herpesviruses: Biology, Therapy, and Immunoprophylaxis. Cambridge: Cambridge University Press (2007).
  7. Boehmer P and Lehman I. “Herpes simplex virus DNA replication”. Annual Review of Biochemistry 66 (1997): 347-384.
  8. Norval M. “Virus–cell interactions”. Medical Microbiology (2012): 82-96.
  9. Friedman H., et al. “Glycoprotein C of herpes simplex virus 1 acts as a receptor for the C3b complement component on infected cells”. Nature 390 (1984): 633-635.
  10. Reubel GH., et al. “Nucleotide sequence of glycoprotein genes B, C, D, G, H and I, the thymidine kinase and protein kinase genes and gene homologue UL24 of an Australian isolate of canine herpesvirus”. Virus Genes2 (2002): 195-200.
  11. Xuan X., et al. “Analysis of canine herpesvirus gB, gC and gD by a recombinant vaccinia virus”. Archives of Virology5 (1997): 1003-1010.
  12. Limbach K., et al. “Nucleotide sequence of the genes encoding the canine herpesvirus gB, gC and gD homologues”. Journal of General Virology 8 (1994): 2029-2039.
  13. Tyack S., et al. “Nucleotide sequence of canine herpesvirus homologues of herpes simplex virus type 1 US2, US3, glycoproteins I and E, US8.5 and US9 genes”. DNA Sequencing (1997): 365-368.
  14. Cargnelutti J., et al. “Outbreaks of canid herpesvirus 1 disease in puppies in southern Brazil”. Pesquisa Veterinaria Brasileira 06 (2015).
  15. Dumon C and Mimouni P. “Mortinatalidad asociada an enfermedades infecciosas en la cría canina: brucelosis, herpesvirosis, micoplasmosis. In: Enciclopedia Veterinaria, Lafont A.; Durieux F. Ed. Elsevier. E-RE 2250 (2005): 1-7.
  16. Rota A., et al. “Seroprevalence of Canine Herpesvirus-1 in Breeding Dogs with or Without Vaccination in Northwest Italy”. Animals7 (2020): 1116.
  17. Erles K., et al. “Longitudinal study of viruses Associated with Canine Infectious Respiratory Disease”. Journal of Clinical Microbiology10 (2004): 4524-4529.
  18. Ronsse V., et al. “Canine herpesvirus-1 (CHV-1): clinical, serological and virological patterns in breeding colonies”. Theriogenology1 (2005): 61-74.
  19. Mullis K and Faloona F. “Specific synthesis of DNA in vitro via a polymerase- catalyzed chain reaction”. Methods of Enzymology 155 (1987): 335-350.
  20. Ghannam MG and Varacallo M. “Biochemistry, Polymerase Chain Reaction. In: StatPearls Internet. Treasure Island (FL): StatPearls Publishing (2021).
  21. Danchin A and Yuek KY. “Bacterial genomics in the study of Virulence”. In SUSSMAN Molecular Medical Microbiology. Barcelona. España. Academic Press (2002): 341-353.
  22. Pennington T. “Electrophoretic typing. In SUSSMAN Molecular medical microbiology”. Barcelona. España. Academic Press (2002): 535-547.
  23. Larenas J., et al. “Primeros antecedentes en Chile de infección por herpes canino con mortalidad neonatal: Mevepa” 1 (1992): 13-16.
  24. Navarro C., et al. “Virus herpes canino en Chile: Propiedades biológicas”. Archivos de Medicina Veterinaria 2 (2005): 133-137.
  25. Fuentes A., et al. “Detection of ul37 gene from Canine Herpes Virus by Polymerase Chain Reaction”. IJMRGE3-4 (2022): 227-237.
  26. Carrasco L., et al. “Detection of the canine herpes virus glycoprotein B gene via Polymerase Chain Reaction. Open Access Research Journal of Multidisciplinary Studies (2022).
  27. Clustal Omega. “Multiple             Sequence             Alignment”         (2021).
  28. Mikloska Z and Cunningham AL. “Herpes simplex virus type 1 glycoproteins gB, gC and gD are major targets for CD4 T-lymphocyte cytotoxicity”. Journal of General Virology pt2 (1989): 353-361.
  29. Arsenakis M., et al. “Expression and regulation of glycoprotein C gene of herpes simplex virus 1”. Journal of General Virology 2 (1986): 367-376.
  30. Rosenthal KL., et al. “Cells Expressing Herpes Simplex Virus Glycoprotein gC but Not gB, gD, or gE Are Recognized by Murine Virus Specific Cytotoxic T Lymphocytes”. Journal of General Virology8 (1987): 2438-2447.

Citation

Citation: Carlos Navarro., et al. “Use of Primer Design to Detect the Glycoprotein C Gene of Canine Herpes Virus by Polymerase Chain Reaction". Acta Scientific Veterinary Sciences 4.3 (2022): 94-101.

Copyright

Copyright: © 2022 Carlos Navarro., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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