Effect of Dietary Probiotics (Lactobacillus acidophilus) on the Growth Performance of Labeo Rohita
Amit Sharma, S Kumar and S.K. Raghuvanshi*
P.G. Department of Zoology, Bareilly College, MJP Rohilkhand University, Bareilly, UP, India
*Corresponding Author: P.G. Department of Zoology, Bareilly College, MJP Rohilkhand University, Bareilly, UP, India
Received:
January 25, 2022; Published: January 31, 2022
Abstract
The present study was carried out to evaluate the influence of dietary supplementation of probiotic bacteria (Lactobacillus acidophilus), on growth performance of Labeo rohita. The probiotic L. acidophilus was procured from the market and the feed was prepared by crushing the basal food with probiotic bacteria in different combinations. The feeding trials were conducted in triplicate for 60 days, to determine the effect of dietary probiotics on growth status of fish. For this, the live fishes (L. rohita) were captured from Sagar Taal, Budaun, U.P. After acclimatization for 10 days, the fishes with similar body weight were distributed randomly into five treatment groups at Department of Zoology, Bareilly College, Bareilly, U.P. These fishes were given basal feed containing L. acidophilus in five concentrations viz., 0.5 (T1), 1.0 (T2), 1.5 (T3), 2.0 (T4) and 2.5 (T5). The control group (T0) was fed basal food only, without L. acidophilus for the same period. Growth was analysed by measuring body length and weight, after the interval of every 15 days (at 15, 30, 45 and 60 days). Two treatment groups (T4 and T5) showed better growth of fishes, than any other treatment group. Group T4 showed a weight gain of 20.51 ± 0.64%, while the percentage length gain was 16.40 ± 0.43%. Similarly, the performance of group T5 was also found good (weight gain 20.22 ± 0.61%, length gain 17.05 ± 0.08%). On the other hand, the results of control treatment were comparatively poor (weight gain 19.50 ± 0.49%, length gain 15.37 ± 0.08%). These results suggest that L. acidophilus can be used as an effective probiotic @ 2% or more in the form of dietary supplement, for L. rohita in aquaculture practices.
Keywords: Feed, Probiotic; Lactobacillus; Growth Performance; Labeo rohita
References
- Javaid MY. “Aquaculture Development in Pakistan". Aquaculture in Asia, Asian Fish Society, India (1990): 291-301.
- Mishra S. “Probiotics: Possible application in aquaculture”. Fish Chimes 1 (2001): 31-37.
- Irianto A and Austin B. “Use of probiotics to control furunculosis in Rainbow trout, Oncorhynchus mykiss (Walbaum)”. Journal of Fish Disease 25 (2002a): 333-342.
- Sahu MK. “Probiotics in aquaculture: importance and future perspectives”. Indian Journal of Microbiology 3 (2008): 299-308.
- Department of Fisheries. “Handbook of Fisheries Statistics”. Ministry of Fisheries, animal husbandry and Dairying, Govt. of India (2015).
- “ICLARM. The World Fish Center annual report” (2001).
- Khan MA., et al. “Growth and body composition of rohu, Labeo rohita (Hamilton), fed compound diet: Winter feeding and rearing to marketable size”. Journal of Applied Ichthyology 20 (2004): 265-270.
- Giri SS. “Effects of dietary supplementation of potential probiotic Bacillus subtilis VSG1 singularly or in combination with Lactobacillus plantarum VSG 3 or/and Pseudomonas aeruginosa VSG 2 on the growth, immunity, and disease resistance of Labeo rohita”. Aquaculture Nutrition 2 (2014): 163-171.
- Fisheries and Aquaculture Information and Statistics Branch (2017).
- Verschuere L. “Probiotic bacteria as biological control agents in aquaculture”. Microbiology and Molecular Biology Reviews 4 (2000): 655-671.
- Taoka Y. “Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system”. Fisheries Science 2 (2006): 310-321.
- Panigrahi A and Azad IS. “Microbial intervention for better fish health in aquaculture: the Indian scenario”. Fish Physiology and Biochemistry 4 (2007): 429-440.
- Ringø E and Gatesoupe FJ. “Lactic acid bacteria in fish: A review”. Aquaculture3-4 (1998): 177-203.
- Nikoskelainen S.. et al. “Characterization of the properties of human- and dairy-derived probiotics for prevention of infectious diseases in fish”. Applied and Environmental Microbiology 6 (2001): 2430-2435.
- Cruce S and Goulet J. “Improving probiotic survival rates”. Food Technology 10 (2001): 36-42.
- Ibrahim F., et al. “Effect of temperature on in vitro adhesion of potential fish probiotics”. Microbial Ecology in Health and Disease 4 (2004): 222-227.
- Yanbo W. and Zirong X. “Effect of probiotics for common carp (Cyprinus carpio) based on growth performance and digestive enzyme activities”. Animal Feed Science and Technology 3-4 (2006): 283-292.
- Chaudhary A and Qazi JI. “Influence of a probiotic Pseudomonas pseudoalcaligenes fermented feed on growth performance of rohu (Labeo rohita) fingerlings”. Punjab University Journal of Zoology 1-2 (2007): 41-56.
- Bagheri T., et al. “Growth, Survival and Gut Microbial Load of Rainbow Trout (Onchorhynchus mykiss) Fry given Diet Supplemented with Probiotic during the Two Months of First Feeding”. Turkish Journal of Fisheries and Aquatic Sciences 8 (2008): 43-48.
- Gatesoupe FJ. “Updating the Importance of Lactic Acid Bacteria in Fish Farming: Natural Occurrence and Probiotic Treatments”. JMMB1-3 (2008): 107-114.
- Suzer C., et al. “Lactobacillus bacteria as probiotics in Gilthead Sea Bream (Sparus aurata, L.) Larvae: Effects on growth performance and digestive enzyme activities”. Aquaculture 280.1-4 (2008): 140-145.
- Essa MA., et al. “Effect of different dietary probiotics on growth, feed utilization and digestive enzymes activities of Nile Tilapia, Oreochromis niloticus”. Journal - The Arab Aquaculture Society 2 (2010): 143-162.
- Faramarzi M., et al. “The Investigation of Lactobacillus acidophilus as Probiotic on Growth Performance and Disease Resistance of Rainbow Trout (Oncorhynchus mykiss)”. American-Eurasian Journal of Scientific Research 1 (2011): 32-38.
- Allameh SK., et al. “Effects of Probiotic Bacteria on Fish Performance”. Advanced Technology in Clinical Microbiology 1 (2017): 2.
- Das N., et al. “Effect of feed probiotic on the growth and their colonization performance on the intestine of rohu (Labeo rohita)”. International Journal of Current Microbiology and Applied Sciences 3 (2020): 806-823.
- Chaudhary A., et al. “Impact of Bacillus subtilis supplemented feed on growth and biochemical constituents in Labeo rohita fingerlings”. Journal of King Saud University - Science 33 (2021).
- Kolndadacha OD., et al. “The role of probiotics in aquaculture in Nigeria- a review”. Canadian Journal of Fisheries and Aquatic Sciences 1 (2011): 8-15.
- Fuller R. “History and development of probiotics”. The Scientific Basis. Chapman and Hall, London (1992): 1-8.
- Gatesoupe FJ. “The use of probiotics in aquaculture”. Aquaculture1-2 (1999): 147- 165.
- Irianto A and Austin B. “Probiotics in aquaculture”. Journal of Fish Diseases 11 (2002b): 633-642.
- Balcazar JL., et al. “Probiotics: a tool for the future of fish and shellfish health management”. Journal of Aquaculture 19 (2004): 239-242.
- Trivedi RK and Goel PK. “Chemical and Biological Methods for Water Pollution Studies”. Environmental Publication, Karad, India Environmental publications (1986).
- APHA AWWA WEF. “Standard methods for the examination of water and wastewater (21st)”, American Public Health Association, American Water Works Association and Water Environment Federation, Washington, D.C., U.S.A. (2005).
- Samantaray K and Mohanty SS. “Interactions of dietary levels of protein and energy on fingerling snakehead, Channa striata”. Aquaculture 156 (1997): 241- 249.
- Venugopal MN and Keshavanath P. “Formulation, stability and keeping quality of three pelleted feeds used in carp culture”. Fishery Technology1 (1984): 18-23.
- Renuakradhya KM and Varghese TJ. “Protein requirement of the carps, Catla catla (Hamiltom) and Labeo rohita (Hamilton)”. Proceedings of the Indian Academy of Sciences 1 (1986): 103-107.
- Nayak SK. “Probiotics and immunity: A fish perspective”. Fish and Shellfish Immunology1 (2010): 2-14.
- Ringo E., et al. “Lactic acid bacteria versus pathogens in the gastrointestinal tract of fish”. Aquaculture Research 41 (2010): 451-467.
- Ghosh K., et al. “Characterization of bacilli isolated from gut of rohu, Labeo rohita, fingerlings and its significance in digestion”. The Journal of Applied Aquaculture 3 (2002): 33-42.
- Fuller AR. “Probiotics in man and animals”. The Journal of Applied Bacteriology5 (1989): 365-378.
- Kennedy SB., et al. “Bacterial management strategies for stock enhancement of warm water marine fish: a case study with common snook (Centropomus undecimalis)”. Bulletin of Marine Science 62 (1998): 573- 588.
- Rengpipat S., et al. “Effects of a probiotic bacterium on black tiger shrimp Penaeus monodon survival and growth”. Aquaculture 167.3-4 (1998): 301-313.
- “Uso de harina de kelp (Macrocystispyrifera) en alimentos para camaron”. Avances en Nutricio ́nAcuı ́cola V-Memorias del Quinto Simposium Internacional deNutricio ́n Acuı ́cola, Me ́rida, Me ́xico 5 (1998): 227-266.
- Singh LA., et al. “Effect of dietary probiotic supplement on growth and survival of Indian major carp seeds”. Asian Journal of Animal Sciences 2 (2014): 174-177.
- Peng L., et al. “Dietary brewer’s yeast and the prebiotic Grobiotic™ AE influence growth performance, immune responses and resistance of hybrid striped bass (Morone chrysops× saxatilis) to Streptococcus iniae infection”. Aquaculture 231 (2004): 445-456.
- Swain SK., et al. “Effect of a probiotics supplements on growth Nutrient utilization and carcass composition in mrigal fry”. Journal of Aquaculture 4 (1996): 29-35.
- Shyamala R., et al. “Physicochemical Analysis of Borewell Water Samples of Telungupalayam Area in Coimbatore District, Tamilnadu, India”. E-Journal of Chemistry 4 (2008): 924-929.
- Pennak RW. “Field and experimental limnology of three Colorado mountain lakes”. Ecology 3 (1968): 505-520.
- Das SM and Pathani SS. “Some physicochemical and biological indicators of pollution in lake Nainital, Kumaun (U.P.)”. Indian Journal of Ecology1 (1978): 7-16.
- Thirupathaiah M., et al. “Analysis of water quality using physico-chemical parameters in lower manair reservoir of Karimnagar district, Andhra Pradesh”. International Journal of Environmental Sciences1 (2012): 172-180.
- Kemker C. “Dissolved oxygen” Fundamentals of Environmental Measurements Fondriest Environmental, Inc (2013).
- George JP. “Aquatic Ecosystem: Structure, Degradation, Strategies for Management”. Recent Advances in Ecobiological Research, M. P (Ed). A. P. H. Publ. House, New Delhi (1997): 603.
- Gatesoupe, F.J. “The effect of three strains of lactic bacteria on the production rate of rotifers, Brachionus plicatilis, and their dietary value for larval turbot, Scophthalmus maximum". Aquaculture 96 (1991): 335-342.
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