Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 2

Protective Effects of Hyssopus Officinalis and Medicago Sativa Extracts in Salmonella-Induced Colitis by Regulating Antioxidant and Inflammatory Mediators

Ehsan SoleimannejaBari1, Hadi Cheraghi2*, Parmida Choubsaz3, Negin Moezzi Nia4 and Mostafa Razmjoo2

1Department of Biology, Faculty of Science, Gazi University, Ankara, Turkey
2Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
3Department of Research and Development, Novin Navand Salamat Pishtaz Co., West Azerbaijan Science and Technology Park, Urmia, Iran
4Department of Biology, Central Tehran Branch, Islamic Azad University, Tehran, Iran

*Corresponding Author: Hadi Cheraghi, Department of Clinical Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran.

Received: December 27, 2021; Published: January 31, 2022


 Background: Salmonella Typhimurium causes gastroenteritis which is characterized by diarrhea. Nowadays, instead of using antibacterial therapies to treat colitis, there is a growing interest in alternative natural products with antibacterial or anti-inflammatory properties. The aim of this study was to investigate the effects of Hyssopus officinalis (Hyssop) and Medicago sativa (Alfalfa), alone and in combination, on salmonella-induced colitis in mice.

Methods and results: Graded doses of 50% ethanolic extracts (25, 50, and 75 mg/kg) for 8 days, showed 50 mg/kg for Hyssop and 75 mg/kg for Alfalfa as an optimal effective dose against Salmonella induced colonic damage score which this dosage was further studies in salmonella-induced colitis for other parameters. Our data showed administration of extracts, not only reduced Lipid Peroxidation, myeloperoxidase activity, and nitric oxide levels but also inhibited decreasing of total antioxidant capacity in the colitis group. Also, following oral administration, the extracts had a significant reduction in pro-inflammatory mediators including interleukin-1β and tumor necrosis factor-α, which had better results in the Mix group. Moreover, the Hyssop and Mix group decreased the histological score and Cox-2 mRNA expression, while Nrf-2 gene expression in the treatment groups did not show a significant change compared to the colitis group.

Conclusions: In conclusion, Oral Hyssop and Alfalfa extracts, alone and in combination, alleviate the symptoms, oxidative stress, and inflammation of salmonella-induced colitis, and combination therapy with Hyssopus officinalis and Medicago sativa may provide a promising dietary approach for the management of acute bacterial colitis.

Keywords: Hyssopus Officinalis; Medicago Sativa; Salmonella; Colitis; Inflammation; Antioxidant


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Citation: Hadi Cheraghi., et al. “Protective Effects of Hyssopus Officinalis and Medicago Sativa Extracts in Salmonella-Induced Colitis by Regulating Antioxidant and Inflammatory Mediators ". Acta Scientific Veterinary Sciences 4.2 (2022): 65-74.


Copyright: © 2022 Hadi Cheraghi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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