Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 4 Issue 2

Clinical Study with Rapid Serological Detection of Rotavirus Infection in Diarrheic Neonatal Calves

Ahmed Abdel-Rady*, Ahmed MA Zaitoun and Zainab MA Youssef

Infectious Diseases, Department of Animal Medicine, Faculty of Veterinary Medicine, Assiut University, Egypt

*Corresponding Author: Ahlam Abd El Halim El Shewy, Dairy Science Department, National Research Centre, El Dokki, Egypt.

Received: December 27, 2021; Published: January 28, 2022

Abstract

Rotavirus is an enteritis causing pathogen in neonatal calves. a total number of 175 neonatal enteric calves were clinically examined. Their feces were sampled to rapidly serotested for Rotavirus detection. The clinical findings on Rotavirus diarrheic calves were anorexia, weakness, arched back with straining during defecation, variable degree of dehydration. Feces were pasty yellowish with and/or without flakes of clotted blood. Body temperature, respiratory and heart rates were varying. The collected fecal samples were serotested by Latex agglutination test (LAT) and Immunochromatographic assay (ICA). Results of LAT and ICA indicated that the positive samples of Rotavirus infection were 9.68% and 8.54%, respectively. It is concluded that Rotavirus infection plays an outstanding role in enteritis of neonatal calves of Assiut Governorate and control measures should be attained. LAT and ICA are effortlessly field serotests in screening of Rotavirus infection in diarrheic neonatal calves.

Keywords: Rotavirus; Calf Diarrhea; LAT; ICA

References

  1. Barua SR., et al. “Comparison of diagnostic tests for detection of bovine Rotavirus A in calf feces”. Macedonian Veterinary Review1 (2021): 1-9.
  2. Zaitoun AMA., et al. “Enteric Rota and Corona viruses' infection in neonatal calves”. Assiut Veterinary Medical Journal156 (2018): 8-17.
  3. El-Sadek FE., et al. “Investigation of Rota and Corona viruses as causative agents for diarrhea in Egyptian calves”. Suez Canal Veterinary Medical Journal2 (2019): 257-272.
  4. Singh S., et al. “Epidemiological study of naturally occurring bovine Rotavirus infection in organized dairy farms, India”. Biological Rhythm Research (2019): 1-9.
  5. Mayameei A., et al. “Evaluation of relationship between Rotavirus and Coronavirus infections with calf diarrhea by capture ELISA”. Comparative Clinical Pathology Journal 19 (2010): 553-557.
  6. Jackson PGG and Cockcroft PD. “Clinical examination of farm animals”. 1st Ed. USA. (2002): 95-101.
  7. Sawant PM., et al. “Molecular characterization of unusual G10P [33], G6P [14] genomic constellations and evidence of zooanthroponosis in bovines”. Infection, Genetics and Evolution (2020): 1-27.
  8. Barua SR. “Clinico-pathology and Molecular characterization of bovine Rotavirus infection in calves in south-eastern part of Bangladesh, PhD”. Thesis, Faculty of Veterinary Medicine, Chittagong Veterinary and Animal Sciences University, Bangladesh (2019).
  9. Brown CC., et al. The Alimentary tract. “In Jubb, Kennedy and Palmer's of Domestic Animals”. Edited by Grant Maxie. 5th Edition. Vol. II, chapter 1. Sunders LTD. (2007): 69-130.
  10. Hassan MN. “G and P typing and sequence analysis of group A Rotavirus in calves and lambs in Kashmir”, PhD. Thesis, Faculty of Post-Graduate Studies Sher-e-Kashmir, University of Agricultural Sciences and Technology of Kashmir, India (2014).
  11. Makwana PM., et al. “Detection of bovine Rotavirus (BRV) infection in neonatal calves of in and around Navsari district of South Gujarat, India”. Journal of Entomology and Zoology Studies2 (2020): 1092-1097.
  12. Okada N and Matsumoto Y. “Bovine Rotavirus G and P types and sequence analysis of the VP7 gene of two G8 bovine Rotaviruses from Japan”. Veterinary Microbiology 84 (2002): 297-305.
  13. Dulgheroff ACB., et al. “Analysis of bovine Rotavirus strains circulating in diarrheic dairy calves in Uberaba”, Minas Gerais, Brazil, during 2008-2009”. Arquivo Brasileiro de Medicina Veterináriae Zootecnia (Brazilian Journal of Veterinary and Animal Science4 (2016): 1090-1094.
  14. Singh TC and Jhala MK. “Comparing Relative Sensitivity and Specificity of LA and RNA-PAGE in Detecting Bovine Rotaviruses”. Buffalo Bulletin1 (2011a): 36-44.
  15. Izzo MM., et al. “Comparisons of three diagnostic techniques for detection of Rotavirus and Coronavirus in calf faeces in Australia”. Australian Veterinary Journal4 (2012): 122-129.
  16. Muhammid HA., et al. “Estimating the sensitivity and Specificity of Reverse transcriptase polymerase chain reaction (RT-PCR) with Rota virus latex agglutination test (Virogen Rotatest) in calves suffering from diarrhea in Karbala province”. Scientific Journal of Medical Research4 (2017): 110-114.
  17. Abdulazeez AA and Abed MN. “Genotyping of Rotavirus in Neonatal Calves with Acute Gastroenteritis in Iraq”. Advances in Microbiology 7 (2017): 863-870.
  18. Sakli GU., et al. “Investigation of bovine Coronavirus and bovine Rotavirus by rapid diagnosis kit and RT-PCR in diarrheic calf feces”. Journal of Istanbul Veterinary Sciences3 (2019): 57-63.
  19. Babalola MO. “Group A Rotavirus genotypes among calf herds in Southwest Nigeria and implications for human Rotavirus vaccines’ efficiency”. Open Journal of Veterinary Medicine 10 (2020): 1-13.

Citation

Citation: Ahmed Abdel-Rady., et al. “Clinical Study with Rapid Serological Detection of Rotavirus Infection in Diarrheic Neonatal Calves". Acta Scientific Veterinary Sciences 4.2 (2022): 29-33.

Copyright

Copyright: © 2022 Ahmed Abdel-Rady., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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