Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 3 Issue 11

Effects of Selenium Nanoparticles-loaded Chitosan Microspheres on Meat Selenium Content and Oxidative Stability in Broiler Chickens

E Giamouri1, S Fortatos1, AC Pappas1, SN Yannopoulos2 and G Papadomichelakis1*

1Laboratory of Nutritional Physiology and Feeding, Department of Animal Science, School of Animal Biosciences, Agricultural University of Athens, Greece
2Foundation for Research and Technology Hellas – Institute of Chemical Engineering Sciences (FORTH/ICE-HT), P.O. Box 1414, GR-26504, Rio-Patras, Greece

*Corresponding Author: George Papadomichelakis, Department of Animal Science, Agricultural University of Athens, Greece

Received: September 20, 2021; Published: October 20, 2021

×

Abstract

Dietary sodium selenite and selenium-yeast are commonly used to enhance the antioxidant defense in broilers, but they can be toxic. A new form of Se nanoparticles stabilized in chitosan microspheres (CS-SeNP) is well established for low toxicity, but their bioavailability and antioxidant potential has not been investigated in broiler feeding. Our objectives were: a) to synthesize and characterize the properties of CS-SeNP and b) to compare the effects of CS-SeNP as a dietary selenium source with those of sodium selenite and selenium-yeast on meat selenium concentration and oxidative stability in broiler chickens. The CS-SeNP were synthesized by chemical reduction and their properties were determined. Four experimental diets were offered to 200 broilers chickens (5 replicates/diet, 10 broilers/replicate); one control (C) with no added Se, and 3 diets supplemented with 0.4 mg Se/kg either from sodium selenite + selenium-yeast (1:1 ratio; T1), selenium-yeast and CS-SeNPs (1:1 ratio; T2) or CS-SeNP alone (T3). Feed intake, weight gain and feed conversion ratio were monitored throughout the trial. At the end of the trial, 10 broilers per diet (2/replicate) were sacrificed and breast meat samples were collected. Meat fatty acid composition was determined by gas chromatography. Meat selenium content and oxidative stability were determined by hydride (vapor) generation atomic absorption spectroscopy and iron-induced lipid oxidation, respectively. Spherical monodispersed CS-SeNPs of 80.5±20 nm average diameter were obtained. The CS-SeNP were exclusively composed of elemental Se and were encapsulated in chitosan, as indicated by the X-ray diffraction and X-ray photoelectron spectroscopy surveys, respectively. Growth performance was not affected by the dietary selenium addition and differences were not observed between the dietary selenium sources. Meat selenium content and oxidative stability were similar in T1, T2 and T3 broilers, but significantly higher (P < 0.05) when compared to C broilers. In conclusion, the selenium from CS-SeNP enriches meat with selenium and enhances meat oxidative stability in a manner similar to the commonly-used inorganic and organic forms. Given the well-established low toxicity, CS-SeNP has a very good potential as a dietary Se source in broilers and should be further studied.

Keywords: Broilers; Oxidative Stability; Selenium; Selenium Nanoparticles-Loaded Chitosan Microspheres; Selenium-yeast; Sodium Selenite

×

References

  1. Ringuet MT., et al. “Analysis of bioavailability and induction of glutathione peroxidase by dietary nanoelemental, organic and inorganic selenium”. Nutrients 13 (2021): 1073.
  2. Korzeniowska M., et al. “Effect of dietary selenium on protein and lipid oxidation and the antioxidative potential of selected chicken culinary parts during frozen storage”. Journal of Chemistry 2018 (2018): 1-12.
  3. Wang CL., et al. “Effects of selenium source and level on growth performance, antioxidative ability and meat quality of broilers”. Journal of Integrative Agriculture1 (2021): 227-235.
  4. Zhao L., et al. “A novel organic selenium compound exerts unique regulation of selenium speciation, selenogenome, and selenoproteins in broiler chicks”. Journal of Nutrition5 (2017): 789-797.
  5. Khurana A., et al. “Therapeutic applications of selenium nanoparticles”. Biomedicine and Pharmacotherapy 1 (2019): 802-812.
  6. Rayman MP. “Selenium intake, status, and health: a complex relationship”. Hormones 19 (2020): 9-14.
  7. Araujo JM., et al. “Delivery of selenium using chitosan nanoparticles: synthesis, characterization, and antioxidant and growth effects in Nile tilapia (Oreochromis niloticus)”. Plos One5 (2021): e0251786.
  8. “Commission Regulation (EC) No 427/2013 of 8 May 2013 concerning the authorisation of selenomethionine produced by Saccharomyces cerevisiae NCYC R646 as a feed additive for all Animal species and amending Regulations (EC) No 1750/2006, (EC) No 634/2007 and (EC) No 900/2009 as regards the maximum supplementation with selenised Yeast”. Official Journal of the European Union, L 127 (2013): 20-22.
  9. Payne RL and Southern LL. “Changes in glutathione peroxidase and tissue selenium concentrations of broilers after consuming a diet adequate in selenium”. Poultry Science8 (2005): 1268-1276.
  10. Cai SJ., et al. “Effects of nano - selenium on performance, meat quality, immune function, oxidation resistance, and tissue selenium content in broilers”. Poultry Science10 (2012): 2532-39.
  11. Samak DH., et al. “Developmental toxicity of carbon nanoparticles during embryogenesis in chicken”. Environmental Science and Pollution Research International16 (2018): 19058-19072.
  12. Shahnawaz K., et al. “Impacts of selenium and vitamin E supplementation on mRNA of heat shock proteins, selenoproteins and antioxidants in broilers exposed to high temperature”. AMB Express1 (2018): 112.
  13. Abdul H., et al. “Green synthesis and characterization of zinc oxide nanoparticles from Ocimum basilicum L. var purpurascens Benth.-lamiaceae leaf extract”. Materials Letters 131 (2014): 16-18.
  14. Li H., et al. “Synthesis and cytotoxicity of selenium nanoparticles stabilized by α-D-glucan from Castanea mollissima Blume”. International Journal of Biological Macromolecules 129 (2019): 818-826.
  15. Hosnedlova B., et al. “Nano-selenium and its nanomedicine applications: a critical review”. International Journal of Nanomedicine 13 (2018): 2107-2128.
  16. Zhang JS., et al. “Elemental selenium at nano size (nano - se) as a potential chemopreventive agent with reduced risk of selenium toxicity: comparison with se - methylselenocysteine in mice”. Toxicology Science1 (2008): 22-31.
  17. Wang H., et al. “Elemental selenium at nano size possesses lower toxicity without compromising the fundamental effect on selenoenzymes: comparison with selenomethionine in mice”. Free Radical Biology and Medicine10 (2007): 1524-1533.
  18. Zhou X and Wang Y. “Influence of dietary nano elemental selenium on growth performance, tissue selenium distribution, meat quality and glutathione peroxidase activity in Guangxi Yellow Chicken”. Poultry Science3 (2011): 680-686.
  19. Saleh AA and Ebeid TA. “Feeding sodium selenite and nano-selenium stimulates growth and oxidation resistance in broilers”. South African Journal of Animal Science1 (2019): 176-184.
  20. Aparna N and Karunakaran R. “Effect of selenium nanoparticles supplementation on oxidation resistance of broiler chicken”. Indian Journal of Science and Technology1 (2016): 1-5.
  21. Li B., et al. “Biogenic selenium and its hepatoprotective activity”. Scientific Reports 7 (2017): 15627.
  22. Bai K., et al. “Preparation and antioxidant properties of selenium nanoparticles-loaded chitosan microspheres”. International Journal of Nanomedicine 12 (2017): 4527-4539.
  23. Ren L., et al. “Preparation and growth-promoting effect of selenium nanoparticles capped by polysaccharide-protein complexes on tilapia”. Journal of the Science of Food and Agriculture2 (2021): 476-485.
  24. Filipović N., et al. “Comparative study of the antimicrobial activity of selenium nanoparticles with different surface chemistry and structure”. Frontiers in Bioengineering and Biotechnology 8 (2021): 1-16.
  25. Bai K., et al. “Selenium nanoparticles‑loaded chitosan/citrate complex and its protection against oxidative stress in d‑galactose‑induced aging mice”. Journal of Nanobiotechnology92 (2017): 1-14.
  26. Pappas AC., et al. “Effects of supplementing broiler breeder diets with organoselenium compounds and polyunsaturated fatty acids on hatchability”. Poultry Science9 (2006): 1584-1593.
  27. O'Fallon JV., et al. “A direct method for fatty acid methylester synthesis: application to wet meat tissues, oils, and feedstuffs”. Journal Animal Science6 (2007): 1511-1521.
  28. Gatellier P., et al. “Effect of diet finishing mode (pasture or mixed diet) on antioxidant status of charolais bovine meat”. Meat Science3 (2004): 385-394.
  29. Terevinto A., et al. “Oxidative status, in vitro iron-induced lipid oxidation and superoxide dismutase, catalase and glutathione peroxidase activities in rhea meat”. Meat Science4 (2010): 706-710.
  30. Sreekumar S., et al. “Parameters influencing the size of chitosan-TPP nano- and microparticles”. Scientific Reports1 (2018): 4695.
  31. Abid S., et al. “Synthesis and characterization of glycol chitosan coated selenium nanoparticles acts synergistically to alleviate oxidative stress and increase ginsenoside content in Panax ginseng”. Carbohydrate Polymers 267 (2021): 118195.
  32. Zhang C., et al. “Synthesis, characterization, and controlled release of selenium nanoparticles stabilized by chitosan of different molecular weights”. Carbohydrate Polymers 134 (2015): 158-166.
  33. Filipović, N., et al. “Poly (e-caprolactone) microspheres for prolonged release of selenium nanoparticles”. Materials Science Engineering 96 (2019): 776-789.
  34. Chung S., et al. “Green synthesized BSA-coated selenium nanoparticles inhibit bacterial growth while promoting mammalian cell growth”. International Journal of Nanomedicine 15 (2020): 115-124.
  35. Dlouhá G., et al. “Effect of dietary selenium sources on growth performance, breast muscle selenium, glutathione peroxidase activity and oxidative stability in broilers”. Czech Journal of Animal Science6 (2008): 265-269.
  36. Zhang Y., et al. “The effect of dietary selenium levels on growth performance, antioxidant capacity and glutathione peroxidase 1 (GSHPx1) mRNA expression in growing meat rabbits”. Animal Feed Science and Technology3-4 (2011): 259-264.
  37. Ebeid TA., et al. “Fortification of rabbit diets with vitamin E or selenium affects growth performance, lipid peroxidation, oxidative status and immune response in growing rabbit”. Livestock Science 2-3 (2013): 323-331.
  38. Papadomichelakis G., et al. “Effects of increasing dietary organic selenium levels on meat fatty acid composition and oxidative stability in growing rabbits”. Meat Science 131 (2017): 132-138.
  39. Hu CH., et al. “Comparative effects of nano elemental selenium and sodium selenite on selenium retention in broiler chickens”. Animal Feed Science and Technology3-4 (2012): 204-210.
  40. Zhang J., et al. “Development, physicochemical characterization and cytotoxicity of selenium nanoparticles stabilized by beta-lactoglobulin”. International Journal of Biological Macromolecules 107 (2018): 1406-1413.
  41. Bai K., et al. “Selenium-nanoparticles-loaded chitosan/chitooligosaccharide microparticles and their antioxidant potential: a chemical and in vivo investigation”. Pharmaceutics 1 (2020): 43.
  42. Zhang JS., et al. “Biological effects of a nano red elemental selenium”. Biofactors1 (2001): 27-38.
  43. Hadrup N., et al. “Effects of 14-day oral low dose selenium nanoparticles and selenite in rat—As determined by metabolite pattern determination”. Peer Journal 4 (2016): e2601.
  44. Pappas AC., et al. “Supranutritional selenium level affects fatty acid composition and oxidative stability of chicken breast muscle tissue”. Journal of Animal Physiology and Animal Nutrition3 (2012): 385-394.
  45. Schäfer K., et al. “Effects of selenium deficiency on fatty acid metabolism in rats fed fish oil-enriched diets”. Journal of Trace Elements in Medicine and Biology1 (2004): 89-97.
×

Citation

Citation: George Papadomichelakis. “Effects of Selenium Nanoparticles-loaded Chitosan Microspheres on Meat Selenium Content and Oxidative Stability in Broiler Chickens". Acta Scientific Veterinary Sciences 3.11 (2021): 27-38.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is December 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"

Contact US