Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 3 Issue 11

Fish Epidermal Mucus from Labeo rohita: Hemolytic Activity and Antibacterial Investigation Against Human Pathogen

M Abareethan*

Assistant Professor, Research Department of Zoology, Government Arts and Science College (Autonomous), Salem, India

*Corresponding Author: M Abareethan, Assistant Professor, Research Department of Zoology, Government Arts and Science College (Autonomous), Salem, India.

Received: September 21, 2021; Published: October 11, 2021

×

Abstract

The fish mucus was extracted from Labeo rohita by using aqueous solution and the chemical composition showed 27.5% of protein, 5.8% of carbohydrate and 0.21% of lipids. The haemolytic activity of the mucus extract showed 43.47 and 37.73 HT/mg in human blood. Further the antibacterial activity of the mucus extract showed against S. aureus, K. pneumoniae, S. typhi, V. cholerae, K. oxytoca, E. coli, V. parahaemolyticus and S. pyogenes were 14, 12, 15, 16, 11, 17, 13 and 12mm at the highest concentration of 100µg/ml. The fish mucus observed MIC values of 100µg/ml against E. coli and the V. cholerae slightly arrested at the above concentration. The fish mucus showed the MBC values of 100µg/ml against E. coli and the V. cholerae slightly arrested at the same concentration. The above antibacterial activity of the mucus suggested as a source antimicrobial agent in future pharmacological for the development of new antimicrobial drugs.

Keywords: Mucus; Biochemical; Antibacterial Activity; MIC; MBC

×

References

  1. Schaeck M., et al. “Fish as a research tool: an alternative to in vivo experiments”. ALTA 41 (2013): 219-229.
  2. Munday PL., et al. “Skin toxins and external parasitism of coral-dwelling gobies”. Journal of Fish Biology 62 (2003): 976-981.
  3. Patel M., et al. “Profiling and role of bioactive molecules from Puntius sophore (Freshwater/Brackish Fish) skin mucus with its potent antibacterial, antiadhesion, and antibiofilm Activities”. Biomolecules 10 (2020): 920.
  4. Villarroel F., et al. “Apolipoprotein A-I, an antimicrobial protein in Oncorhynchus mykiss: evaluation of its expression in primary defence barriers and plasma levels in sick and healthy fish”. Fish and Shellfish Immunology 23 (2007): 197-209.
  5. Lee Y., et al. “The antibacterial activity of fish skin mucus with various extraction solvents and their in-vitro evaluation methods”. International aquatic research 12 (2020): 1-21.
  6. Ebran N., et al. “Isolation and characterization of novel glycoproteins from fish epidermal mucus: Correlation between their pore-forming properties and their antibacterial activities”. Biochimica et Biophysica Acta 1467 (2000): 271-280.
  7. Nair MSR. “Fish skin toxins. In hand book of natural toxins; Marine toxins and venoms”. Tu, A.T., Ed.; Marcel Dekker: New York, NY, USA 3 (1988).
  8. Masso-Silva J and Diamond G. Antimicrobial peptides from fish”. Pharmaceuticals 7 (2014): 265–310.
  9. Rajanbabu V and Chen JY. “Applications of antimicrobial peptides from fish and perspectives for the future”. Peptides 32 (2011): 415-420.
  10. Beck BH and Peatman E. “Mucosal Health in Aquaculture”. 1st ed.; Academic Press: New York, NY, USA, 2015.
  11. Wei OY., et al. “Screening of antibacterial activity of mucus extract of snakehead fish, Channa straitus (Bloch)”. European Review for Medical and Pharmacological Sciences 14 (2010): 675-681.
  12. Bragadeeswaran S and Thangaraj S. “Hemolytic and antibacterial studies on skin mucus of eel fish, Anguilla linnaues 1758”. Asian Journal Biological Sciences3 (2011): 272-276.
  13. Tyor AK and Kumari S. “Biochemical characterization and antibacterial properties of fish skin mucus of fresh water fish, Hypophthalmichthys nobilis". International Journal of Pharmacy and Pharmaceutical Sciences6 (2016): 132-136.
  14. Bradford MM. “A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding”. Analytical Biochemistry (1-2) (1976): 248-254.
  15. Dubois M., et al. “Colorimetric method for determination of sugars and related substances”. Annals of Chemistry 28 (1956): 350-356.
  16. Folch JM., et al. “A simple method for isolation and purification of total lipids from animal tissue”. Journal of Biological Chemistry 226 (1957): 497-499.
  17. Seedevi P., et al. “Structural characterization and biomedical properties of sulphated polysaccharide from the gladius of Sepioteuthis lessoniana (Lesson, 1831)”. International Journal of Biological macromolecules 85 (2016): 117-125.
  18. Manivasagan P., et al. “Study on the proteinaceous gel secretion from the skin of the catfish, Arius maculates (Thunberg, 1792)”. African Journal of Biotechnology 8 (2009): 7125-7129.
  19. Dhotre MA., et al. “Extraction, Biochemical characterization and antibacterial activity of fish mucus”. Indian Streams Research Journal12 (2013): 1-8.
  20. Bragadeeswaran S., et al. “Antimicrobial and hemolytic activity of fish epidermal mucus Cynoglossus arel and Arius caelatus”. Asian Pacific Journal of Tropical Medicine 4 (2011): 305-309.
  21. Rajaganapathi J. “Studies on antibacterial activity on five marine molluscs”. Sc., Dissertation, Annamalai University, India (1996): 43.
  22. Prem Anand T., et al. “Antibacterial activity of marine mollusks from Porto Novo region”. Indian Journal of Marine Sciences 26 (1997): 206-208.
  23. Selva Prabhu A., et al. “Antibacterial activity of Ascidian phallusia arabica against human clinical isolates”. Journal of Applied Pharmaceutical Science10 (2011): 143-145.
  24. Ramasamy P., et al. “Screening of antibacterial drugs from marine gastropod Chicoreus ramosus (Linnaeus, 1758)”. Journal of Coastal Life Medicine3 (2013): 181-185.
  25. Rao V., et al. “Defense properties in the epidermal mucus of different freshwater fish species”. AACL Bioflux2 (2015): 184-194.
  26. Kuppulakshmi C., et al. “Antibacterial properties of fish mucus from Channa punctatus and Cirrhinus mrigala”. European Review for Medical and Pharmacological Sciences 12 (2008): 149-153.
  27. Subramanian S., et al. “Comparison of antimicrobial activity in the epidermal mucus extracts of fish”. Comparative Biochemistry and Physiology - Part B: Biochemistry and Molecular Biology 150 (2008): 85-92.
  28. Lee Y., et al. “The antibacterial activity of fish skin mucus with various extraction solvents and their in-vitro evaluation methods”. International Aquatic Research 12 (2020): 1-21
  29. Hisar O., et al. “In vitro antimicrobial and antifungal activities of aqueous skin mucus from rainbow trout (Oncorhynchus mykiss) on human pathogens”. Marne Science and Technology Bulletin 3 (2014): 19-22.
×

Citation

Citation: M Abareethan. “Fish Epidermal Mucus from Labeo rohita: Hemolytic Activity and Antibacterial Investigation Against Human Pathogen". Acta Scientific Veterinary Sciences 3.11 (2021): 15-20.




Metrics

Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In





News and Events


Contact US