Compare Culture and Designed ELISA Methods to Diagnose Tuberculosis in Suspected Pigeons
Mahsa Lari Baghal1, Mansour Mayahi2*, Nader Mosavary3 and Zahra Boroomand2
1Ph.D. Candidate in Avian Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Bovine Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran
*Corresponding Author: Mansour Mayahi, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.
September 01, 2021; Published: September 20, 2021
Backgrounds: Avian Tuberculosis (TB) is a chronic disease and caused by Mycobacterium avium subsp. avium and Mycobacterium genavense.
Aims: To diagnose the avian tuberculosis disease by culture and designed Elisa methods and compare results of two diagnostic methods.
Methods: One hundred one suspected pigeons were selected based on clinical signs and poor physical condition and transferred to the Avian Diseases Laboratory of Veterinary Medicine Faculty of Ahvaz. Blood sample was collected via pigeon wing vein and serum was collected. Then pigeons were euthanized and post mortem was performed, any gross lesions were recorded and sample from liver and spleen and each organ with gross lesions was collected and stored in freezer at -40 °C. All samples were placed on dry ice and transferred to reference tuberculosis laboratory of Razi Vaccine and Serum Research Institute, Karaj. Each sample after preparation inoculated into glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein-Jensen medium and Herrold-egg yolk medium and bacterial growth was monitored for 3 months. Antibody against mycobacterium was were measured by designed Elisa test.
Results: Out of 101 pigeons, 39 (38.6%) and 13 pigeons (12.9%) were tuberculosis positive respectively by culture and designed Elisa methods. Correlation between results of culture and designed Elisa methods was significant (0.485) (p < 0.01).
Conclusions: It was concluded that mycobacterium affected pigeons lungs and there is significant correlation (0.485) (p < 0.01) between culture and designed ELISA system tests. The ELISA test due to easy sampling, speed, low price, non-invasive, prefer test for screening affected pigeons flocks.
Keywords: Avian Tuberculosis; Culture; Designed Elisa Test; Mycobacterium
- Asadollahi P K., et al. “Genotyping of Mycobacterium avium subsp. avium isolates from naturally infected lofts of domestic pigeons in Ahvaz by IS901 RFLP”. Iranian Journal of Microbiology 5 (2015): 260-264.
- Boulfion M. “Molecular Identification of Mycobacteria Isolated from Pigeons Submitted to Razi Institute”. Postgraduate Thesis from Azad University Research Science Branch, Tehran, No. 5469 (2009).
- Christal GP. “Implications of Mycobacterium in Clinical Disorders”. Chapter 28 (2013): 681-689.
- FM. “Antituberculous immunity: new solutions to an old problem”. Reviews of Infectious Diseases 13.5 (1991): 940-950.
- Cromie R., et al. “Susceptibility of captive wild fowl to avian tuberculosis: the importance of genetic and environmental factors”. Tubercle 72 (1994): 105-109.
- Cromie RL., et al. “Avian immune responses to Mycobacterium avium: the wildfowl example”. Developmental and Comparative Immunology 2-3 (2000): 169-185.
- Darrel K and Styles M. Avian Tuberculosis: The Disease and Its Management (2008).
- Dhama K., et al. “Tuberculosis in birds: Insight into the Mycobacterium avium infections”. Veterinary Medicine International (2011): 1-14.
- Dvorska L., et al. “A standardised restriction fragment length polymorphism (RFLP) method for typing Mycobacterium avium isolates links IS901 with virulence for birds”. Journal of Microbiological Methods1 (2003): 11-22.
- Fulton RM and Thoen CO. “Tuberculosis”. in Diseases of Poultry, Saif, YM; Barnes, HJ; Glisson, JR; Fadly, AM; McDougald, LR and Swayne, DE. Eds., Iowa State University Press, Ames, IA, USA (2003): 836-844.
- Fulton R and Sanchez S. “Tuberculosis”. In: Saif, YM.; Fadly, AM.; Glisson, JR.; McDougald, LR.; Nolan, LK.; Swayne, DE. Disease of poultry, 12th ed. Blackwel publishing (2008): 940-941.
- Gonzalez M., et al. “Outbreak of avian tuberculosis in 48-week-old commercial layer hen flock”. Avian Diseases 46 (2002): 1055-1061.
- Haidarnejad O., et al. “Sero-prevalence of subclinical paratuberculosis (johne's disease) in dairy farms of tehran-iran using absorbed elisa assay”. Comparative Pathobiology 3.58 (2017): 2239-2246.
- Hines MK., et al. “Mycobacterial infections of animals, Pathology and pathogenesis”. Laboratory Animal Science4 (1995): 334-351.
- Hoop RK., et al. “Etiological agents of mycobacteriosis in pet birds between 1986 and1995”. Journal of Clinical Microbiology4 (1996): 991-992.
- Horsburgh CR., et al. “Environmental risk factors for acquisition of Mycobacterium avium complex in persons with human immunodeficiency virus infection”. The Journal of Infectious Diseases 2 (1994): 362-367.
- Jabbarzadeh E and Seifi M. “Rapid identification of different Mycobacterium species in tuberculosis”. Journal of Clinical Veterinary Research1 (2010): 5-13.
- Jorgensen JP. “Serological investigation of strains of Mycobacterium avium and Mycobacterium intracellulare isolated from animal and non-animal sources”. Nordisk veterinaermedicin 4-5 (1978): 150-162.
- Kent PT and Kubica GP. “Public health mycobacteriology: a guide for the level III laboratory”. USDHHS. Centers for Disease Control, Atlanta (1985).
- Kriz P., et al. “Mycobacterium avium subsp. avium in domestic pigeons (Columba livia f. domestica) diagnosed by direct conventional multiplex PCR: a case report”. Veterinary Medicine2 (2010): 87-90.
- Kul O., et al. “An out break of avian tuberculosis in peafowl (Pavo cristatus) and pheasants (phasianus colchicus) in a zoological aviary in Turkey”. Veterinary Medicine Czech, 50.10 (2005): 446-450.
- Marco I., et al. “Mycobacterium infection in a captive-reared Capercaillie (Tetrao urogallus)”. Avian Disease1 (2000): 227-230.
- Mayahi M., et al. “Comparison of four different culture media for growth of Mycobacterium avium subsp. avium isolated from naturally infected lofts of domestic pigeons”. Iran Journal of Microbiology4 (2013): 379-382.
- Miguel D., et al. “Mycobacteriosis in naturally infected ring-neck doves (Streptopelia risoria): investigation of the association between feather colour and susceptibility to infection, disease and lesions type”. Avian Pathology4 (2008): 443-450.
- Movassaghi AR., et al. “Granulomatous dermatitis caused by Mycobacterium Spp. in pigeons (first report)”. 4th Iranian Congress of Clinical Microbiology (2010): 667.
- Nouri M., et al. “Cutaneous Tuberculosis, Coccidiosis, Cestodia Infection and Trichoepithelioma in a Crown of Domestic Pigeons (Columba livia). Second National Congress of Veterinary Laboratory Sciences 251 (2012).
- Pavlas M., et al. “Diagnosis of avian tuberculosis-mycobacteriosis by rapid agglutination”. Acta Veterinaria Brno 1 (1992): 63-69.
- Pesek L. “Zoonotic Diseases (Birds -Humans): Avian Tuberculosis, Bird to Human Transmission”. Pet Bird Magazine (1998).
- Tell LA., et al. “Mycobacteriosis in birds. revue scientifique et technique (international office of epizootics), 20.1 (2001): 180-203.
- Tell LA., et al. “Avian tuberculosis in birds”. Revue Scientifique et Technique1 (2001): 180-203.
- Tell LA., et al. “Diagnosis of avian mycobacteriosis; comparison of culture, acid-fast stains, and polymerase chain reaction for the identification of Mycobacterium avium in experimentally inoculated Japanese quail (Coturnix coturnix japonica)”. Avian Disease2 (2003): 444-452.
- Thoen CO. “Tuberculosis”. In: Calnek BW (eds.): Diseases of Poultry. 10th ed. Iowa State University Press, Ames, Iowa, USA (1997).
- Thoen CO., et al. “Enzyme- linked immunosorbent assay for detecting antibodies in swine infected with Mycobacterium avium”. American Journal of Veterinary Research 8 (1979): 1096-1099.
- Thorel MF., et al. “Mycobacterium avium infections in animals”. Veterinary Research5 (1997): 439-447.
- VanDerHeyden N. “Mycobacterial infections: new strategies in the treatment of avian tuberculosis”. Seminars in Avian Exotic Pet Medicine1 (1997): 25-33.
- Velayati AA. “Tuberculosis disease”. volume one, first edition, University Publication Center (1977): 1-20.