Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 3 Issue 10

Compare Culture and Designed ELISA Methods to Diagnose Tuberculosis in Suspected Pigeons

Mahsa Lari Baghal1, Mansour Mayahi2*, Nader Mosavary3 and Zahra Boroomand2

1Ph.D. Candidate in Avian Health and Diseases, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
2Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran
3Bovine Reference Laboratory, Razi Vaccine and Serum Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran

*Corresponding Author: Mansour Mayahi, Department of Clinical Sciences, Faculty of Veterinary Medicine, Shahid Chamran University of Ahvaz, Ahvaz, Iran.

Received: September 01, 2021; Published: September 20, 2021

Abstract

Backgrounds: Avian Tuberculosis (TB) is a chronic disease and caused by Mycobacterium avium subsp. avium and Mycobacterium genavense.

Aims: To diagnose the avian tuberculosis disease by culture and designed Elisa methods and compare results of two diagnostic methods.

Methods: One hundred one suspected pigeons were selected based on clinical signs and poor physical condition and transferred to the Avian Diseases Laboratory of Veterinary Medicine Faculty of Ahvaz. Blood sample was collected via pigeon wing vein and serum was collected. Then pigeons were euthanized and post mortem was performed, any gross lesions were recorded and sample from liver and spleen and each organ with gross lesions was collected and stored in freezer at -40 °C. All samples were placed on dry ice and transferred to reference tuberculosis laboratory of Razi Vaccine and Serum Research Institute, Karaj. Each sample after preparation inoculated into glycerinated Lowenstein-Jensen medium, pyruvate-enriched Lowenstein-Jensen medium and Herrold-egg yolk medium and bacterial growth was monitored for 3 months. Antibody against mycobacterium was were measured by designed Elisa test.

Results: Out of 101 pigeons, 39 (38.6%) and 13 pigeons (12.9%) were tuberculosis positive respectively by culture and designed Elisa methods. Correlation between results of culture and designed Elisa methods was significant (0.485) (p < 0.01).

Conclusions: It was concluded that mycobacterium affected pigeons lungs and there is significant correlation (0.485) (p < 0.01) between culture and designed ELISA system tests. The ELISA test due to easy sampling, speed, low price, non-invasive, prefer test for screening affected pigeons flocks.

Keywords: Avian Tuberculosis; Culture; Designed Elisa Test; Mycobacterium

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Citation

Citation: Mansour Mayahi., et al. “Compare Culture and Designed ELISA Methods to Diagnose Tuberculosis in Suspected Pigeons". Acta Scientific Veterinary Sciences 3.10 (2021): 33-40.

Copyright

Copyright: © 2021 Mansour Mayahi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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