Naqa Saleh Mahdi Tamimi1*, Amir Rostami2 and Hossein Esmaeili3
1Department of Internal and preventive Medicine; College of veterinary medicine, University of Wasit. Kut, Iraq
2Department of Internal Medicine; Faculty of Veterinary Medicine; University of Tehran, Tehran, Iran
3Department of Microbiology; Faculty of Veterinary Medicine; University of Tehran, Tehran, Iran
*Corresponding Author: Naqa Saleh Mahdi Tamimi, Department of Internal and preventive Medicine; College of veterinary medicine, University of Wasit. Kut, Iraq. E-mail: email@example.com, firstname.lastname@example.org
Received: July 28, 2021; Published: August 31, 2021
Reptiles are shown to harbor and shed Salmonella without having any sign of illness and with notable rates that could pose a health hazard for their owners. In order to find a suitable enrichment broth for the recovery of Salmonella from these animals’ feces Rappaport Vassiliadis (RV) broth was compared with selenite F (SF). Two sets of samples were collected from 270 reptiles in Tehran province which belonged to 46 reptilian species of 22 families. The specimens included 171 cloacal swabs, 20 feces, and 79 terrarium water specimens (in which the reptile defecates) according to their availability and reptile species. Each sample was separately enriched in RV and SF enrichment broths and then inoculated in three culture media to increase the possibility of Salmonella detection (CHROMagar Salmonella, MacConkey agar, and xylosine-lysin desoxycholate) and later checked for their biochemical properties as Salmonella spp. A total of 147 (54.4%) specimens were found positive for Salmonella using both enrichment broths. The positivity rate for RV (49.6%) was significantly higher than SF (40.4%) showing that RV could be considered a better enrichment broth for Salmonella recovery in feces or cloacal swabs (p = 0.031). In addition, the reptilian feces samples were more likely to have positive culture results than cloacal swabs and terrarium water in RV and SF broths (p = 0.001 and 0.000, respectively); suggesting that, feces can be better specimens for detection of Salmonella intestinal shedding in reptiles or other animals.
Keywords: Enrichment Broth; Reptile; Salmonella
Citation: Naqa Saleh Mahdi Tamimi., et al. “Comparison Between Two Enrichment Broths for Detection of Salmonella from Reptilian Feces and Cloacal Swabs". Acta Scientific Veterinary Sciences 3.9 (2021): 82-85.
Copyright: © 2021 Naqa Saleh Mahdi Tamimi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.