Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 3 Issue 10

Ameliorating Effect of Terminalia arjuna Against 5-Fluorouacil Induced Cardiotoxicity in Wistar Rats

Sushma Ghadigaonkar1*, A Gopala Reddy2, BDP Kala Kumar3 and M Lakshman4

1Assistant Professor, Department of Veterinary Pharmacology and Toxicology, Mumbai Veterinary College, Mumbai, MAFSU, Nagpur, India
2Professor and University Head and Registrar of PV Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, India
3Professor, Department of Veterinary Pharmacology, C.V. Sc., Rajendranagar, India
4Professor and University Head, Department of Veterinary Pathology, C. V. Sc, Rajendranagar, Hyderabad, India

*Corresponding Author: Sushma Ghadigaonkar, Assistant Professor, Department of Veterinary Pharmacology and Toxicology, Mumbai Veterinary College, Mumbai, MAFSU, Nagpur, India.

Received: July 15, 2021; Published: August 26, 2021

Abstract

The Terminalia arjuna has traditionally been used as cardiotonic. The aim of the study was to evaluate the protective effect of aqueous extract of Terminalia arjuna on pathological changes after 5-FU administration. Wistar rats where randomly divided into 6 groups: normal control, TRLE alone, Captopril alone, 5-FU alone, 5-FU + TALE and 5-FU + Captopril. Animals were sacrificed and tissue samples from the cardiac muscle were taken and processed for thickness of left ventricle wall measurement, myocardial antioxidant parameters and also for both Light and Electron Microscopical examination. 5-FU significantly altered all the parameters in the study while animals received TALE and Captopril along with 5-FU revealed significant improvement in all the parameters and the values were comparable to the control group. Light microscopic examination revealed that administration of 5-FU caused variable signs of cardiotoxicity which are represented by dilatation of cardiac muscle fiber, focal atrophy of cardiomyocyte with severe interstitial edema, and dilated blood vessels. Ultrastructural examination of these specimens confirmed the light microscopic findings and demonstrated irregular nuclear envelope, dilated myofibrils with degenerating nucleus, dilatation of nucleus, dilatation of sarcoplasmic reticulum, disorganized, electron-dense and bizarre shaped mitochondria and congestion of blood capillaries. Pre-treatment with TALE and its concomitant administration with 5-FUfor 14 days attenuated 5-FU induced myocardial damage and effectively reverted the abnormal structural changes near to normalcy. In conclusion, these results suggest that Terminalia arjuna has a protective potential in ameliorating 5-FU induced cardiotoxicity.

Keywords: Terminalia arjuna; WHO; Captopril; Wistar Rats

References

  1. World Health Organization (WHO): Who reveals leading cause of death and disability worldwide 2000-2019, News release, Geneva (2020).
  2. Gharib I and Burnett A K. “Chemotherapy induced cardiotoxicity: current practices and prophylaxis”. European Journal of Heart Failure 4 (2002): 235-242.
  3. Boveli D., et al. “Cardiotoxicity of chemotherapeutic agent and radiotherapy related heart disease”. Annals of Oncology 21 (2010): 277-282.
  4. Van Dalen EC., et al. “Cardioprotective intervention for cancer patients receiving anthracyclines”. Cochrane Library: Cochrane Reviews 2 (2011): cd003917.
  5. Sorrentino M F., et al. “5-Fluorouracil induced cardiotoxicity: review of the literature”. Cardiology Journal 19 (2012): 453-458.
  6. Stewart T., et al. “Cardiotoxicity with 5-Fluorouracil and capecitabine: more than just vasospastic angina”. Internal Medicine Journal 40 (2010): 3030-307.
  7. Molinaro M., et al. “Recent advances on pathophysiology, diagnostic and therapeutic insight in cardiac dysfunction induced by antineoplastic drugs”. Biomedical Research International (2015): 138-148.
  8. Lenihan D J and Cardinale D M. “Late cardiac effects of cancer treatment”. Journal of Clinical Oncology 30 (2012): 3657-3666.
  9. Kalam K and Marwick T H. “Role of cardioprotective therapy for prevention of cardiotoxicity with chemotherapy”. European Journal of Cancer 49 (2013): 2900-2909.
  10. Sterba M., et al. “Oxidative stress, redox signaling and metal chelation in anthracyclines cardiotoxicity and pharmacological cardioprotection”. Antioxidant and Redox Signaling 18 (2013): 899-929.
  11. Alter P., et al. “Cardiotoxicity 5-Fluorouracil”. Cardiovascular and Hematological Agents Medicinal Chemistry 4 (2006): 1-5.
  12. Alvarez P., et al. “5-Fluororuracil derivative: a patent review”. Expert Opinion on Therapeutic Patents 22 (2012): 107-123.
  13. Tyler V and Premila M S. “Ayurvedic Herbs: A clinical guide to the healing plants of traditional Indian Medicine” (2006).
  14. Yesodharan K and Sujana K A. “Ethnomedical knowledge among Malamalasar tribe of Parambikulam wildlife sanctuary Kerala”. Indian Journal of Traditional Knowledge3 (2006): 481-485.
  15. Chandan K., et al. “Phytochemical properties of total antioxidant status of acetone and methanol extract of Terminalia arjuna Roxb bark and its hypoglycemic effect on Type-II diabetic albino rats”. Indian Journal of Pharmaceutical Science 2 (2013): 199-201.
  16. Hala F Abd-Ellah. “Ameliorative effect of Captopril against 5-Fluorouracil induced cardiotoxicity in rats: A study with light and electron microscopes”. Journal of Applied Sciences Research 8 (2012): 863-872.
  17. Batta H and Abd E L Azim. “Biochemical studies of Captopril against 5-Fluorouracil induced heart toxicity in rats”. International Journal of Advanced Research 3 (2015): 247-321.
  18. Shin Chang Yong., et al. “Synergistic decrease in blood pressure by Captopril combined with Losartan in spontaneous hypertensive rats”. Archives of Pharmaceutical Research6 (2009): 955-962.
  19. Singh C., et al. “Evaluation of antioxidant activity of Terminalia arjuna leave extract”. Pharmacologyonline 1 (2011): 998-1006.
  20. Bozzollo J J and Russel L D. “Electron Microscopy: Principle and technique for biologists”. Jones and Bartlett, Boston (1999): 670.
  21. Eman T M and Ghada M S. “Evaluation of cardioprotective activity of Leptidium sativum seed powder in albino rats treated with 5-Fluorouracil”. Beni-Suef University of Basic and Applied Science 5 (2012): 208-215.

Citation

Citation: Sushma Ghadigaonkar., et al. “Ameliorating Effect of Terminalia arjuna Against 5-Fluorouacil Induced Cardiotoxicity in Wistar Rats". Acta Scientific Veterinary Sciences 3.10 (2021): 75-79.

Copyright

Copyright: © 2021 Sushma Ghadigaonkar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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