Ameliorating Effect of Terminalia arjuna Against 5-Fluorouacil Induced Cardiotoxicity in Wistar Rats
Sushma Ghadigaonkar1*, A Gopala Reddy2, BDP Kala Kumar3 and M Lakshman4
1Assistant Professor, Department of Veterinary Pharmacology and Toxicology, Mumbai Veterinary College, Mumbai, MAFSU, Nagpur, India
2Professor and University Head and Registrar of PV Narsimha Rao Telangana Veterinary University, Rajendranagar, Hyderabad, India
3Professor, Department of Veterinary Pharmacology, C.V. Sc., Rajendranagar, India
4Professor and University Head, Department of Veterinary Pathology, C. V. Sc, Rajendranagar, Hyderabad, India
*Corresponding Author: Sushma Ghadigaonkar, Assistant Professor, Department of Veterinary Pharmacology and Toxicology, Mumbai Veterinary College, Mumbai, MAFSU, Nagpur, India.
July 15, 2021; Published: August 26, 2021
The Terminalia arjuna has traditionally been used as cardiotonic. The aim of the study was to evaluate the protective effect of aqueous extract of Terminalia arjuna on pathological changes after 5-FU administration. Wistar rats where randomly divided into 6 groups: normal control, TRLE alone, Captopril alone, 5-FU alone, 5-FU + TALE and 5-FU + Captopril. Animals were sacrificed and tissue samples from the cardiac muscle were taken and processed for thickness of left ventricle wall measurement, myocardial antioxidant parameters and also for both Light and Electron Microscopical examination. 5-FU significantly altered all the parameters in the study while animals received TALE and Captopril along with 5-FU revealed significant improvement in all the parameters and the values were comparable to the control group. Light microscopic examination revealed that administration of 5-FU caused variable signs of cardiotoxicity which are represented by dilatation of cardiac muscle fiber, focal atrophy of cardiomyocyte with severe interstitial edema, and dilated blood vessels. Ultrastructural examination of these specimens confirmed the light microscopic findings and demonstrated irregular nuclear envelope, dilated myofibrils with degenerating nucleus, dilatation of nucleus, dilatation of sarcoplasmic reticulum, disorganized, electron-dense and bizarre shaped mitochondria and congestion of blood capillaries. Pre-treatment with TALE and its concomitant administration with 5-FUfor 14 days attenuated 5-FU induced myocardial damage and effectively reverted the abnormal structural changes near to normalcy. In conclusion, these results suggest that Terminalia arjuna has a protective potential in ameliorating 5-FU induced cardiotoxicity.
Keywords: Terminalia arjuna; WHO; Captopril; Wistar Rats
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