Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Research Article Volume 3 Issue 9

Comparative Antimicrobial Activity of Aspirin, Paracetamol, Flunixin Meglumine, Tolfenamic Acid, Diclofenac Sodium and Pheniramine Maleate

Bhoj R Singh*, BS Pruthvishree, Akanksha Yadav, Ravichandran Karthikeyan, Obli R Vinodhkumar and Dharmendra K Sinha

Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, India

*Corresponding Author: Bhoj R Singh, Head, Division of Epidemiology, ICAR-Indian Veterinary Research Institute, Izatnagar, India.

Received: July 27, 2021; Published: August 18, 2021


The study was conducted to evaluate the antimicrobial activity of aspirin, paracetamol, flunixin meglumine, tolfenamic acid and diclofenac sodium and pheniramine maleate on 499 strains of microbes of 117 species belonging to 26 genera of Gram-negative (G-ve), nine genera of Gram-positive (G+ve) bacteria and four strains of two Candida species. A total of 92.79%, 44.09%, 54.91% and 30.26% bacterial strains were sensitive to 2.56 mg/mL aspirin, 3.2 mg/mL flunixin, 2.56 mg/mL diclofenac and 1.28 mg/mL meloxicam, respectively. For paracetamol and pheniramine maleate only one strain of Aerococcus species was sensitive at ≤ 3.2 mg/mL concentration of these drugs and none of the strains was susceptible to tolfenamic acid even at 10.28 mg/mL. The analysis indicated that G+ve bacteria had significantly lower susceptibility to aspirin (OR = 0.30; CI99 = 0.12 - 0.78) but higher susceptibility to flunixin (OR = 7.22; CI99 = 4.12-12.50) and diclofenac (OR = 1.91; CI99 = 1.15 - 3.15) than G-ve bacteria. There was no significant difference in meloxicam susceptibility of G+ve and G-ve bacteria. The study concluded that NSAIDs and pheniramine maleate may not be used as antimicrobials in therapeutically achievable systemic concentrations of the drugs within biological safety limits of plasma concentration. However, the scope of use of NSAIDs still exists in form of non-antibiotic topical antimicrobial preparations.

Keywords: NSAIDs; Antihistaminics; Antibacterials; Antibiotics; Drug-repurposing; Alternative-antimicrobials


  1. Lima R., et al. “Prospects for the use of new technologies to combat multidrug-resistant bacteria”. Frontiers in Microbiology 10 (2019): 692.
  2. Lagadinou M., et al. “Antimicrobial properties on non-antibiotic drugs in the era of increased bacterial resistance”. Antibiotics 3 (2020): 107.
  3. González-Bello C. “Antibiotic adjuvants - A strategy to unlock bacterial resistance to antibiotics”. Bioorganic and Medicinal Chemistry Letters 27 (2017): 4221-4228.
  4. Kumar S., et al. “An overview on mechanisms and emergence of antimicrobials drug resistance”. Advances in Veterinary Science 1 (2S) (2013): 7-14.
  5. Chan E.W.L., et al. “Synergistic effect of non-steroidal anti-inflammatory drugs (NSAIDs) on antibacterial activity of cefuroxime and chloramphenicol against methicillin-resistant Staphylococcus aureus”. Journal of Global Antimicrobial Resistance 10 (2017): 70-74.
  6. Wang C., et al. “Defeating antibiotic-resistant bacteria: Exploring alternative therapies for a post-antibiotic era”. Internationl Journal of Molecular Sciences3 (2020): 1061.
  7. Gajdács, M. “Non-antibiotic pharmaceutical agents as antibiotic adjuvants”. Acta Biologica Szegediensis1 (2020): 17-23.
  8. Miró-Canturri A., et al. “Drug repurposing for the treatment of bacterial and fungal infections”. Frontiers in Microbiology41 (2019).
  9. Singh B.R. et al. “Potential of herbal antibacterials as an alternative to antibiotics for multiple drug resistant bacteria: An Analysis”. Research Journal of Veterinary Sciences1 (2020): 1-9.
  10. Singh BR. “Evaluation of antibacterial activity of Salvia officinalis [L.] Sage oil on veterinary clinical isolates of bacteria”. Noto-are Medicine 15782463 (2013): 2013-11-22.
  11. Kruszewska H., et al. “Search of antimicrobial activity of selected non-antibiotic drugs”. Acta Poloniae Pharmaceutica 6 (2002): 436-439.
  12. Singh BR. “Mitigating antimicrobial resistance with aspirin (acetylsalicylic acid) and paracetamol (acetaminophen): Conversion of doxycycline and minocycline resistantbacteria into sensitive in presence of aspirin and paracetamol”. bioRxiv Preprint (2021).
  13. Singh BR. “Labtop for Microbiology Laboratory”. Berlin: Lambert Academic Publishing (2009).
  14. Carter GR. “Diagnostic Procedures in Veterinary Microbiology”. Springfield: Charles C. Thomas (1975).
  15. Holt JG., et al. “Bergey's Manual of Determinative Bacteriology”. 9th ed. Baltimore: Williams and Wilkins (1994).
  16. David T., et al. “Statistical Analysis—Specific Statistical Tests: Indications for Use”. Eds. Wiley W. Souba, Douglas W. Wilmore, Surgical Research, Academic Press (2001): 1201-1215.
  17. Audigier V., et al. “Multiple imputation for categorical variables with multiple correspondence analysis”. Statistics and Computing 27 (2017): 501-518.
  18. Gocmen JS., et al. “In vitro antibacterial activity of some systemic and topical antihistaminic preparations”. Clinical and Investigative Medicine6 (2009): E232.
  19. Verma AK., et al. “To assess antimicrobial action of paracetamol”. Acta Scientific Microbiology8 (2020): 65-70.
  20. Papich MG. “Flunixin meglumine”. In: Saunders Handbook of Veterinary Drugs. 4th Edn (2016): 336-338.
  21. King CS., et al. “Fatal perforating intestinal ulceration attributable to flunixin meglumine overdose in rats”. Laboratory Animals2 (1997): 205-208.
  22. Salem-Milani A., et al. “Antibacterial effect of diclofenac sodium on Enterococcus faecalis”. Journal of Dentistry (Tehran, Iran) 1 (2013): 16-22.
  23. Abd El-Baky RM., et al. “Effect of non-steroidal anti-inflammatory drugs and dexamethazone on the biofilm formation and expression of some adhesion-related genes of Candida albicans and Staphylococcus aureusi”. African Journal of Microbiology Research20 (2016): 694-707.
  24. Swan GE., et al. “Toxicity of diclofenac to Gyps vultures”. Biology Letters2 (2006): 279-282.
  25. Hunter LJ., et al. “The patterns of toxicity and management of acute nonsteroidal anti-inflammatory drug (NSAID) overdose”. Open Access Emerging Medicine 3 (2011): 39-48.
  26. Smolinske S.C., et al. “Toxic effects of nonsteroidal anti-inflammatory drugs in overdose. An overview of recent evidence on clinical effects and dose-response relationships”. Drug Safety 5.4 (1990): 252-274.
  27. Lehmann H.A., et al. “Meloxicam: A toxicology overview”. Inflammopharmacology 4 (1996): 105-123.
  28. She P., et al. “Meloxicam inhibits biofilm formation and enhances antimicrobial agents efficacy by Pseudomonas aeruginosa”. Microbiology Open1 (2018): e00545.
  29. Zimmermann P., et al. “Antimicrobial effects of antipyretics”. Antimicrobial Agents and Chemotherapy 61 (2017): e02268-2316.
  30. Zimmermann P., et al. “The effect of aspirin on antibiotic susceptibility”. Expert Opinion on Therapeutic Targets 11 (2018): 967-972.
  31. Wang WH., et al. “Aspirin inhibits the growth of Helicobacter pylori and enhances its susceptibility to antimicrobial agents”. Gut 52 (2003): 490-495.
  32. Price CT. et al. “Increases in the mutation frequency at which fusidic acid-resistant Staphylococcus aureus arise with salicylate”. Journal of Medical Microbiology 50 (2001): 104-106.


Citation: Bhoj R Singh., et al. “Comparative Antimicrobial Activity of Aspirin, Paracetamol, Flunixin Meglumine, Tolfenamic Acid, Diclofenac Sodium and Pheniramine Maleate". Acta Scientific Veterinary Sciences 3.9 (2021): 30-42.


Copyright: © 2021 Bhoj R Singh., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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