Beenu Jain1*, Anuj Tewari2, Minaksi Prasad3, Prakash S Dhamannapatil4 and Surinder Kumar Kadian1
1Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India
2Department of Veterinary Microbiology, College of Veterinary and Animal Sciences, GBPUAT, Pantnagar, Uttarakhand, India
3Department of Animal Biotechnology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, Haryana, India
4Division of Animal Biotechnology, National Dairy Research Institute, Karnal, India
*Corresponding Author: Beenu Jain, Department of Veterinary Microbiology, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar, India.
Received: October 20, 2020; Published: November 21, 2020
Foot-and-mouth disease (FMD) is a highly contagious disease of cloven-hoofed animals and wild ruminants. It is caused by the foot-and-mouth disease virus (FMDV). Due to the contagiousness and aerosol transfer of FMDV, not all labs can handle FMDV. Only labs equipped with proper biocontainment facility (BSL3) can handle the work related to live FMDV. This may create hindrance in the expedition of sample testing by virus isolation. Thus, it is imperative to make the existing BSL-2 labs competent for testing the FMDV infected samples. Real-time PCR is one of the rapid ways of testing FMDV infected samples. Positive control of FMDV RNA is required for performing this assay. However, due to biosecurity reasons live FMDV cannot be used in BSL-2 labs. Therefore, one of the ways of obtaining FMDV RNA is extracting FMDV RNA from FMDV inactivated vaccine for its subsequent use in real-time PCR. In this work, we have extracted the FMDV RNA from inactivated FMDV vaccine and have subsequently used it as a positive control for screening oro-pharyngeal fluid from field animals (buffaloes) to confirm the FMDV carrier state. This work explores new opportunities to strengthen existing BSL -2 labs for testing the FMDV infected samples without virus isolation.
Keywords: Foot-and-mouth Disease Virus; Vaccine; qRT-PCR; Positive Control; FMDV RNA
Citation: Beenu Jain., et al. “Utility of Vaccine Extracted FMDV RNA as a Positive Control in Real-time PCR for the Detection of FMDV Carrier Animals". Acta Scientific Veterinary Sciences 3.2 (2021): 25-31.
Copyright: © 2021 Beenu Jain., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.