Abstract

  The aim of the study was to evaluate methanolic extracts from the stem and leaves of Petiveria alliacea, bark of Bursera simaruba and Casearia corymbosa collected in two seasons on eggs of Ancylostoma caninum, Haemonchus placei and cyathostomin. The egg hatch inhibition assay was used at concentrations of 3600, 2400, 1200, 600 and 300 µg/ml. The extracts with high activity were also evaluated at 300, 150, 75 and 37.5 µg/ml. Lethal concentrations were determined at 50% (LC₅₀) and 99% (LC₉₉), as well as the confidence intervals at 95%. Differences (p < 0.05) between control and evaluated concentrations were analyzed. The P. alliacea extract collected in the rainy season (CRS) showed a percentage of egg hatch inhibition (PEHI) ≥ 91.6% from 150 µg/ml, and ovicidal effect (≥ 90.1%) from 150 µg/ml with stem and 300 µg/ml with leaves in both parameters on the three genera of gastrointestinal nematodes evaluated. The B. simaruba extract CRS (3600 µg/ml) showed a PEHI of 95.4, 25.4 and 56.3% against A. caninum, H. placei and cyathostomins, respectively. While the C. corymbosa extract at the same season and concentration had the highest PEHI of 55.1, 74.0 and 56.4% against the three nematodes, respectively. The effect of B. simaruba and C. corymbosa on the eggs was the failure of the L₁ larvae to hatch (23.7 - 95.1% and 30.4 - 60.8%, respectively, at 3600 µg/ml). Additionally, it was observed that C. corymbosa extract caused morphological damage to the larvae that hatched (100% from 1200 µg/ml). Extracts from the stem of P. alliacea CRS showed the lowest LC₅₀ (33.3, 78.9 y 68.6 µg/ml) and LC₉₉ (79.5, 178.0 and 277.4 µg/ml) against A. caninum, H. placei and cyathostomins, respectively. It is concluded that the methanolic extracts of P. alliacea, B. simaruba and C. corymbosa collected in rainy season showed the highest anthelmintic activity on eggs of A. caninum, H. placei and cyathostomins. The stem of P. alliacea CRS has high ovicidal activity on the three nematodes, representing a potential alternative control with a broad spectrum against the main nematodes of domestic animals.

Keywords: Control Alternatives; Gastrointestinal Nematodes; Methanolic Extracts; Petiveria alliacea; Bursera simaruba; Casearia corymbosa

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Citation

Citation: José Alberto Rosado-Aguilar., et al. “Anthelmintic Activity of Petiveria alliacea, Bursera simaruba y Casearia corymbosa Collected in Two Seasons on Ancylostoma caninum, Haemonchus placei and Cyathostomins". Acta Scientific Veterinary Sciences 2.12 (2020): 12-24.

Copyright

Copyright: © 2020 José Alberto Rosado-Aguilar., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.