Abstract

  The study was aimed to evaluate the effect of exposure of pregnant female CD-1 mice to standard cigarettes’ smokes on histo-pathological changes of 10 days old juvenile lungs tissues at the cellular level. A total of 30 female CD-1 mice were randomly divided into three sets of 10 mice each and placed in a special modified smoking machine over the experimental period. The first set were exposed to fresh air (control group); while the mice in the other two sets were exposed to tobacco smokes from standard filter cigarettes for 2 (group 2) and 3 (group 3) weeks, respectively. The fumes from these cigarette brands contain nicotine, tar and carbon monoxide. The mice in the exposed set groups were in contact with the fumes from 6 cigarettes for two exposure of 15 minutes per day. At the end of the experiment, mice were decapitated under deep anesthesia, and the lungs were dissected under stereomicroscope. Histological results showed that exposure of mice to tobacco smokes for three weeks caused significant histological changes in the lungs epithelium which include epithelial cells proliferation, cilia disruption as well as presence of inclusion bodies. Alveolar wall thickening, collapsed alveoli and blood extravasations were also observed. These structural changes constitute great index that nicotine induced these changes, similar to the changes observed when experiencing long term tissues irritation. This study showed that prenatal tobacco smokes had a negative effect on gaseous exchange in alveoli and predispose to neoplastic changes.

Keywords: Tobacco Smokes; Lungs; Histo-Morphological; CD-1 Mice; Embryo

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Citation

Citation: Issa S Al-Amri., et al. “Effects of Prenatal Tobacco Smoke on Lungs Histo-Morphological Changes of CD-1 Mice at Embryo Developmental Stage". Acta Scientific Veterinary Sciences 2.12 (2020): 02-08.

Copyright

Copyright: © 2020 Issa S Al-Amri., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.