Acta Scientific Veterinary Sciences (ISSN: 2582-3183)

Review Article Volume 2 Issue 10

A Brief Review of the Next Generation Sequencing

Afnan Saleem*

PhD Scholar, Division of Animal Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-Kashmir, India

*Corresponding Author: Afnan Saleem, PhD Scholar, Division of Animal Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, SKUAST-Kashmir, India.

Received: August 26, 2020; Published: September 16, 2020



 Since the discovery of DNA in 1869, the development of classical genetics and research of how our bodies work have all led us to this point in human history where we are competent enough to completely sequence all the genes in a living body. The nature of any biological inquiry has now been transformed by the high throughput sequencing technologies to generate genome scale data sets. Based on Sanger’s founding discovery of DNA sequencing, next generation DNA sequencing platforms are rapidly evolving to exem-plify an elegant interplay between biology and technology. Over the past few years, second and third generation sequencing tech-nology has dramatically accelerated the comprehensive analysis of biological and biomedical research. This review enlists a short summary of second and third generation sequencing technologies with an overview of the advances in the sequencing platforms.

Keywords: Next Generation Sequencing; Platforms; Second Generation; Third Generation; Applications



  1. Sanger F., et al. “DNA sequencing with chain-terminating inhibitors”. Proceedings of the National Academy of Sciences of the United States of America 74 (1977): 5463-5467.
  2. Wheeler D., et al. “The complete genome of an individual by massively parallel DNA sequencing”. Nature 452 (2008): 872-876.
  3. Margulies M., et al. “Genome sequencing in microfabricated high-density picolitre reactors”. Nature 437 (2005): 376-380.
  4. Metzker ML. “Sequencing technologies - The next generation”. Nature Reviews Genetics 11 (2010): 31-46.
  5. Shendure J and Ji H. “Next-generation DNA sequencing”. Nature Biotechnology 26 (2008):1135-1145.
  6. Liu L., et al. “Comparison of next-generation sequencing systems”. Journal of Biomedicine and Biotechnology (2012): 251364.
  7. McCoy RC., et al. “Illumina TruSeq synthetic long-reads empower de novo assembly and resolve complex, highly-repetitive transposable elements”. PLoS One 9 (2014): e106689.
  8. McKernan KJ., et al. “Sequence and Structural Variation in a Human Genome Uncovered by Short-Read, Massively Parallel Ligation Sequencing Using Two-Base Encoding”. Genome Research 19 (2009): 1527-1541.
  9. Guzvic M. “The history of DNA sequencing”. Journal of Medical Biochemistry 32 (2013): 301-312.
  10. Rothberg JM., et al. “An integrated semiconductor device enabling non-optical genome sequencing”. Nature 475 (2011): 348-352.
  11. Diekstra A., et al. “Translating Sanger-based routine DNA diagnostics into generic massive parallel ion semiconductor sequencing”. Clinical Chemistry 61 (2015): 154-162.
  12. Eid J., et al. “Real-time DNA sequencing from single polymerase molecules”. Science 323 (2009): 133-138.
  13. Hart C., et al. “Single-molecule sequencing: sequence methods to enable accurate quantitation”. Methods in Enzymology 472 (2010): 407e430.
  14. Schadt EE., et al. “A window into third-generation sequencing”. Hum Mol Gene 19 (2010): R227-R240.
  15. Bayley H. “Nanopore sequencing: From imagination to reality”. Clinical Chemistry 61 (2015): 25-31.
  16. Stoddart D., et al. “Single nucleotide discrimination in immobilized DNA oligonucleotides with a biological nanopore”. Proceedings of the National Academy of Sciences of the United States of America 106 (2009): 7702-7707.


Citation: Afnan Saleem. “A Brief Review of the Next Generation Sequencing”.Acta Scientific Veterinary Sciences 2.10 (2020): 02-05.


Acceptance rate35%
Acceptance to publication20-30 days
Impact Factor1.008

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is June 25, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US