Acta Scientific Veterinary Sciences (ASVS)(ISSN: 2582-3183)

Research Article Volume 2 Issue 7

Ovine Tunica Albuginea as Xenograft for Cystoplasty in Rats

Natasha Nogueira Ferreira1, Cecília Ribeiro Castañon2, Fellipe Ferreira Lemos de Medeiros1, Fernanda Moreira da Silva3, Bruna Scalzilli1, Tábata Maués3*, Carla Ferreira Farias Lancetta4, Viviane Alexandre Nunes Degani4 and Maria de Lourdes Gonçalves Ferreira5

1Postgraduate Program in Clinic and Animal Breeding, Universidade Federal Fluminense, Niterói, RJ, Brazil
2Department of Morphology, Universidade Castelo Branco, Rio de Janeiro, RJ, Brazil
3Professor, Firmino Mársico Filho Veterinary Teaching Hospital, Universidade Federal Fluminense, Niterói, RJ, Brazil
4Department of Morphology, Universidade Federal Fluminense, Niterói, RJ, Brazil
5Department of Clinical Pathology, Universidade Federal Fluminense, Niterói, RJ, Brazil

*Corresponding Author: Tábata Maués, Professor, Firmino Mársico Filho Veterinary Teaching Hospital, Universidade Federal Fluminense, Niterói, RJ, Brazil.

Received: June 20, 2020; Published: June 30, 2020



  The purpose of this study was to prove the conserved ovine tunica albuginea (OTA) as a practicable and adequate biomaterial for bladder scaffolds even as its histopathological cicatrization aspects. The conserved heterologous OTA implant was experimentally used for cystoplasty in 20 Wistar rats. The operated bladder areas of all rats from test (n = 20) and simulation (n = 20) groups were examined macroscopically and histologically at 7, 14, 2, 8 and 42 postoperative days. Test group animals underwent partial cystectomy followed by cystoplasty with OTA graft application, and simulation group rats to partial cystectomy. There was no mortality in any group, and all animals showed good post-surgery recovery. Bladders histopathological analysis showed that the test group obtained more intensive blood vessels and had the first signs of total regeneration earlier than the simulation group. Our findings pointed that macroscopic and histological results, easiness of surgical technique and graft availability, OTA can be used as an alternative biomaterial graft for bladder wall reconstruction in rats which represent a valuable animal model to comparative studies with the human being and other species.

Keywords:Biomaterials; Bladder; Cystoplasty; Scaffold; Tissue Engineering



  1. Greca FH., et al. “Retalho de submucosa de intestino delgado autólogo para aumento da capacidade vesical da bexiga: estudo experimental em cães”. Revista do Colégio Brasileiro de Cirurgiões 29 (2002): 294-299.
  2. Zhang Y., et al. “Challenges in a larger bladder replacement with cell-seeded and unseeded small intestinal submucosa grafts in a subtotal cystectomy model”. BJU International 98 (2006): 1100-1105.
  3. Jack GS., et al. “Urinary bladder smooth muscle engineered from adipose stem cells and a three-dimensional synthetic composite”. Biomaterials 30 (2009): 3259-3270.
  4. Jin C., et al. “A Lipid-Nanosphere-Small MyoD Activating RNA-Bladder Acellular Matrix Graft Scaffold [NP(saMyoD)/BAMG] Facilitates Rat Injured Bladder Muscle Repair and Regeneration [NP(saMyoD)/BAMG]”. Frontiers in Pharmacology 11 (2020): 1-12.
  5. Pope JC., et al. “The ontogeny of canine small intestinal submucosa regenerated bladder”. The Journal of Urology 158 (1997): 1105-1110.
  6. Voyti-Harbin SL., et al. “Identification of extractable growth factors from small intestinal submucosa”. Journal of Cellular Biochemistry 67 (1997): 478-491.
  7. Portis AJ., et al. “Laparoscopic augmentation cystoplasty with different biodegradable grafts in an animal model”. The Journal of Urology 164 (2000): 1405-1411.
  8. Lin HK., et al. “Biomatrices for bladder reconstruction”. Advanced Drug Delivery Reviews 82 (2015): 47-63.
  9. Mathur RK., et al. “Tunica albuginea urethroplasty for anterior urethral strictures: A urethroscopic analysis”. International Journal of Urology 16 (2009): 751-755.
  10. Queiroz FF., et al. “Ensaio biomecânico da túnica albugínea bovina conservada em glicerina 98% para utilização como membrana biológica”. Ciência Rural 42 (2012): 501-506.
  11. Oliveira LL., et al. “Avaliação histológica da túnica albugínea bovina como biomaterial conservada em glicerina a 98% e em glutaraldeído a 0,625%”. Revista Brasileira de Medicina Veterinária 37 (2015): 309-315.
  12. Canellas ACC., et al. “Emprego de Túnica albugínea heteróloga ovina como reforço de parede abdominal em ratos”. Pesquisa Veterinária Brasileira 37 (2017): 1108-1112.
  13. Moraes TA., et al. “Morpho-fuctional evaluation of porcine tunica albuginea as a graft for cystoplasty in rats”. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 69 (2017): 9116-9118.
  14. Hsu GL., et al. “Anatomy and Strength of the Tunica Albuginea: Its Relevance to Penile Prosthesis Extrusion”. The Journal of Urology 151 (1994): 1205-1208.
  15. Junqueira LC. and Carneiro J. “Histologia Básica”. 13th edition. Rio de Janeiro: Guanabara Koogan (2017): 554.
  16. Tolosa EMC., et al. “Manual de Técnicas Para Histologia Normal e Patológica”. 1 ed. São Paulo: Manole (2003): 331p.
  17. Albrecht R., et al. “Aplicação de cola de fibrina autóloga e cola de N-Butil-cianoacrilato com implante de pericárdio suíno em cistoplastia experimental em coelhos (Oryctolagus cuniculus)”. Revista Portuguesa de Ciências Veterinárias 110 (2015): 215-220.
  18. Pokrywczynska M., et al. “Is the Poly (L-Lactide-co-Caprolactone) nanofibrous membrane suitable for urinary bladder regeneration?”. PLoS ONE 9 (2014): e105295.
  19. Iijima K., et al. “Transplantation of preserved human amniotic membrane for bladder augmentation in rats”. Tissue Engineering 13 (2007): 513-524.
  20. Zhou Z., et al. “Adipose-derived stem-cell-implanted poly(e-caprolactone)/chitosan scaffold improves bladder regeneration in a rat model”. Regenerative Medicine 13 (2018): 331-342.
  21. Pinto Filho STL., et al. “Avaliações clínica, ecográfica e anatomofisiológica do alotransplante parcial de vesícula urinária com células-tronco mesenquimaisalogênicas derivadas do tecido adiposo em coelhos”. Arquivo Brasileiro de Medicina Veterinária e Zootecnia 67 (2015): 1304-1312.
  22. Wongsetthachai P., et al. “Urinary bladder wall substitution using autologous tunica vaginalis in male dogs”. Research in Veterinary Sciences 90 (2011): 156-159.
  23. Zhao Y., et al. “Time-dependent bladder tissue regeneration using bilayer bladder acellular matrix graft-silk fibroin scaffolds in a rat bladder augmentation model”. Acta Biomaterialia 23 (2015): 91-102.
  24. Kosan M., et al. “Tissue reactions of suture materials (polyglactine 910, chromed catgut and polydioxanone) on rat bladder wall and their role in bladder stone formation”. Urological Research 36 (2008): 43-49.
  25. Xia D., et al. “Immunomodulatory response of layered small intestinal submucosa in a rat bladder regeneration model”. Journal of Biomedical Materials Research 107 (2019): 1960-1969.
  26. Wang Y., et al. “Bioengineered bladder patches constructed from multilayered adipose-derived stem cell sheets for bladder regeneration”. Acta Biomaterialia 85 (2019): 131-141.


Citation: Tábata Maués., et al. “Ovine Tunica Albuginea as Xenograft for Cystoplasty in Rats".Acta Scientific Medical Sciences 2.7 (2020): 56-61.


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