Abstract

  Avian influenza isolated from different Egyptian governorate during the period of 2010-2017 have been computationally evaluated for the B-cell and T-cell epitope mapping and MHC II binding sequence prediction. The ten studied isolates showed limited variation on the level of deduced amino acid for the N1 gene, on the other hand H5 gene showed much wider variation. Many B – and T- cell epitopes have been predicted for both H5 and N1 proteins which spanning nearly the entire sequence. B- Cell epitopes have been seen within the cleavage site thus the generated antibody clone might hinder the cleavage of haemagglutinin by the cellular protease and prevent viral entry. Different T- cell epitopes found on the N1 protein can stimulate IFN-γ production and hence inhibit viral replication. These data explain the power of genetic vaccine coding for both H5 and N1 to elucidate high protection rate with minimal shedding level.

Keywords:Avian Influenza; Haemagglutinin Gene; Neuraminidase Gene; Highly Pathogenic; Cleavage Site

References

Citation

Citation: Soliman YA., et al. “Computational and Molecular Characterization of Surface Proteins Neuraminidase and Haemagglutinin from Egyptian Isolates of Avian Influenza H5N1 Subtype". Acta Scientific Medical Sciences 2.5 (2020): 08-14.

Copyright

Copyright: © 2020 Soliman YA., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.