Acta Scientific Veterinary Sciences (ASVS)(ISSN: 2582-3183)

Research Article Volume 2 Issue 5

Computational and Molecular Characterization of Surface Proteins Neuraminidase and Haemagglutinin from Egyptian Isolates of Avian Influenza H5N1 Subtype

Soliman YA1*, Maha AN Gamal1 and Eman MS El-Nagar2

1Central Laboratory for Evaluation of Veterinary Biologics, Abassia, Cairo, Egypt
2Veterinary Serum and vaccine research institute, Abassia, Cairo, Egypt

*Corresponding Author: Soliman YA, Central Laboratory for Evaluation of Veterinary Biologics, Abassia, Cairo, Egypt.

Received: April 16, 2020; Published: April 29, 2020

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Abstract

  Avian influenza isolated from different Egyptian governorate during the period of 2010-2017 have been computationally evaluated for the B-cell and T-cell epitope mapping and MHC II binding sequence prediction. The ten studied isolates showed limited variation on the level of deduced amino acid for the N1 gene, on the other hand H5 gene showed much wider variation. Many B – and T- cell epitopes have been predicted for both H5 and N1 proteins which spanning nearly the entire sequence. B- Cell epitopes have been seen within the cleavage site thus the generated antibody clone might hinder the cleavage of haemagglutinin by the cellular protease and prevent viral entry. Different T- cell epitopes found on the N1 protein can stimulate IFN-γ production and hence inhibit viral replication. These data explain the power of genetic vaccine coding for both H5 and N1 to elucidate high protection rate with minimal shedding level.

Keywords:Avian Influenza; Haemagglutinin Gene; Neuraminidase Gene; Highly Pathogenic; Cleavage Site

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References

  1. Kruy SL Buisson and Y Buchy P. “Asia: avian influenza H5N1”. Bulletin de la Société de Pathologie Exotique 101 (2008): 238-242.
  2. Wan H and Perez DR. “Quail carry sialic acid receptors compatible with binding of avian and human influenza viruses”. Virology 346 (2006): 278-286.
  3. Luke CJ and Subbarao K. “Vaccines for pandemic influenza”. Emerging Infectious Diseases 12 (2006): 66-72.
  4. Santhakumar D., et al. “Avian Interferons and Their Antiviral Effectors”. Frontiers in Immunology 8 (2017): 49-66.
  5. Eman MS El-Nagar. “Study of the immune potentiation effect of Esat-6 of Mycobacteria on DNA vaccine encoding the H5 gene of avian influenza virus”. PhD thesis, Veterinary. faculty, Cairo university (2014).
  6. Maha AN Gamal. “Production and Evaluation of DNA vaccine coding for Neuraminidase N1 gene against avian influenza H5N1”. PhD thesis, Veterinary. faculty, Alexandria university (2016).
  7. Soliman YA., et al. “Generation of Plasmid Vector Coding for Neuraminidase Gene NA1 of Highly Pathogenic Avian Influenza H5N1 Subtype”. Alexandria Journal of Veterinary Sciences2 (2016): 101-111.
  8. Sambrook J., et al. “Molecular cloning. A Laboratory Manual, 2nd Edition, Cold Spring Harbor Lab. N.Y (1989).
  9. Wang P., et al. “Peptide binding predictions for HLA DR, DP and DQ molecules”. BMC Bioinformatics 11 (2010): 568-575.
  10. Wang P., et al. “A systematic assessment of MHC class II peptide binding predictions and evaluation of a consensus approach”. PLOS Computational Biology 4 (2008): e1000048.
  11. Horimoto T., et al. “Cleavability of heamagglutinin from an extremely virulent strain of avian influenza virus containing a unique cleavage site sequence”. Journal of Veterinary Medical Science 5 (1995): 927-930.
  12. Subbarao K., et al. “Characterization of an avian influenza A (H5N1) virus isolated from a child with a fatal respiratory illness”. Science 279 (1998): 393-396.
  13. Zambon MC. “The pathogenesis of influenza in humans”. Reviews in Medical Virology 4 (2001): 227-241.
  14. Carrat F and Flahault A. “Influenza vaccine: the challenge of antigenic drift”. Vaccine 25 (2007): 6852-6862.
  15. Colman PM., et al. “Structure of the catalytic and antigenic sites in influenza virus neuraminidase”. Nature5912 (1983): 41-44.
  16. Sylte MJ and Suarez DL. “Influenza Neuraminidase as a Vaccine Antigen”. Current Topics in Microbiology and Immunology 333 (2009): 227-241.
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Citation

Citation: Soliman YA., et al. “Computational and Molecular Characterization of Surface Proteins Neuraminidase and Haemagglutinin from Egyptian Isolates of Avian Influenza H5N1 Subtype". Acta Scientific Medical Sciences 2.5 (2020): 08-14.




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