Emmagouni Sharath Kumar Goud, Mamta Pandey, Prashant Singh, Chhama Singh, Vedamurthy Gowdar Veerappa, Dheer Singh, Suneel Kumar Onteru*
Molecular Endocrinology, Functional Genomics, and Systems Biology Lab, Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, India
*Corresponding Author: Suneel Kumar Onteru, Senior Scientist, Molecular Endocrinology, Functional Genomics, and Systems Biology Lab, Animal Biochemistry Division, ICAR-National Dairy Research Institute, Karnal, India.
Received: February 06, 2020; Published: February 21, 2020
Post-partum reproductive disorders are of major concern in buffaloes. Mainly these are regulated by steroidogenic proteins, such as CYP17, CYP19, and 3β-HSD. These enzymes are involved in the synthesis of several steroid hormones, an imbalance in the levels of which can lead to the causation of several reproductive illnesses ultimately affecting milk production. In the present in-silico study, we analyzed the structural details of the three steroid enzymes in terms of their physiochemical properties, N- and O-glycosylation sites, phosphorylation sites, secondary structure, surface probability, hydrophilicity and antigenic index. Additionally, the 3D models for the three proteins were enacted in the SWISS-MODEL online tool and the models were assessed by RAMPAGE. It was found that the CYP17 protein sequence of buffalo is small having 247 aminoacids when compared with other species. All the three buffalo steroid proteins were having more than 95% similarity with cattle, sheep and goat, except human. The amino acids responsible for the heme-binding site in CYP17, a catalytic site in CYP19, and 3β-HSD proteins in buffalo were found to be conserved when compared with human protein sequences. The 3D models predicted for the three buffalo steroidogenic proteins were found to be of good quality through Ramachandran plot. Further, the individual phylogenetic trees for each of the protein showed that human proteins are phylogenetically outgroup to buffalo proteins. Overall, the in-silico analysis of the three buffalo steroidogenic proteins could prove to arise new insights for resolving reproductive disorders in the buffaloes.
Keywords: Steroidogenic; In-Silico; Buffalo; Steroid
Citation: Suneel Kumar Onteru. “An In-Silico Structural Characterization of the Buffalo Steroidogenic Proteins". Acta Scientific Veterinary Acta Scientific Veterinary Acta Scientific Medical Sciences 2.3 (2020): 01-08.
Copyright: © 2020 Suneel Kumar Onteru. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.