Acta Scientific Pharmaceutical Sciences (ASPS)(ISSN: 2581-5423)

Review Article Volume 6 Issue 3

A Review on Qualitative and Quantitative Analysis of Carbohydrates Extracted from Bacteria

Shukla Pooja, Meghani Sonali, Patel Charmi, Shah Dhrumi, Rathod Zalak R* and Saraf Meenu S

Department of Microbiology and Biotechnology, School of Science, Gujarat University, Gujarat, India

*Corresponding Author: Rathod Zalak R, Department of Microbiology and Biotechnology, School of Science, Gujarat University, Gujarat, India.

Received: January 28, 2022; Published: February 22, 2022


Carbohydrate analysis is significant for a variety of reasons. To determine the compositions of foods, beverages, and their constituents, qualitative and quantitative analysis is used. Ingredient labels with precise compositional information are ensured via qualitative examination. Quantitative analysis guarantees that added components on ingredient labels are listed in the correct order. Quantitative analysis also ensures that the amounts of specific consumer-interest components, such as -glucan, are correct and that caloric content can be calculated. To authenticate (i.e., detect adulteration of) food ingredients and products, both qualitative and quantitative analyses can be applied. Developing a carbohydrate analysis system that can be utilized across animal species could improve the utility of analytes and the amount of data we can collect on carbohydrate dietary effects. Sugars, fructans, insoluble fibre, and soluble fibre are some nutritionally important groups of carbohydrates that may be useful to evaluate based on quantities present in diets and apparent impacts on animal performance.

Keywords:Analysis; Carbohydrate; DNSA (3, 5, dinitrosalicylic acid); Reducing Sugar; Total Carbohydrate


  1. Başkan K S., et al. “Spectrophotometric total reducing sugars assay based on cupric reduction”. Talanta 147 (2016): 162-168.
  2. BeMiller J N. “Carbohydrate analysis”. In Food analysis. Springer, Boston, MA (2010): 147-177.
  3. Benedict S R. “A reagent for the detection of reducing sugars”. Journal of Biological Chemistry5 (1909): 485-487.
  4. Brummer Y and Cui S W. “Understanding carbohydrate analysis”. Food Carbohydrates: Chemistry, Physical Properties and Applications (2005): 1-38.
  5. Daniels R., et al. “The Fehling and Benedict tests”. Journal of Chemical Education4 (1960): 205.
  6. Doutre D A., et al. “Spectrophotometric methods to determine carbohydrates in soil”. Soil Biology and Biochemistry6 (1978): 457-462.
  7. Ebell L F. “Variation in total soluble sugars of conifer tissues with method of analysis”. Phytochemistry1 (1969): 227-233.
  8. Elzagheid, M. I. “Laboratory activities to introduce carbohydrates qualitative analysis to college students”. World Journal of Chemical Education2 (2018): 82-86.
  9. Fleischer H. “The Iodine Test for Reducing Sugars–A Safe, Quick and Easy Alternative to Copper (II) and Silver (I) Based Reagents”. World 2 (2019): 45-52.
  10. Foulger J H. “The use of the Molisch (α-naphthol) reactions in the study of sugars in biological fluids”. Journal of Biological Chemistry2 (1931): 345-353.
  11. Garriga M., et al. “Determination of reducing sugars in extracts of Undaria pinnatifida (harvey) algae by UV-visible spectrophotometry (DNS method)”. E INNOVACIÓN EN Ing (2017): 444.
  12. Hall MB. “Methodological challenges in carbohydrate analyses”. Revista Brasileira de Zootecnia 36 (2007): 359-367.
  13. Katoch R. “Carbohydrate Estimations”. In Analytical Techniques in Biochemistry and Molecular Biology. Springer, New York, NY (2011): 67-76.
  14. Malherbe J S and Meyer C J. “A mini-qualitative carbohydrate analysis session”. Journal of Chemical Education11 (1997): 1304.
  15. Masuko T., et al. “Carbohydrate analysis by a phenol–sulfuric acid method in microplate format”. Analytical Biochemistry1 (2005): 69-72.
  16. Mishra T K. “General Tests of Carbohydrates and their Identification”. Elsevier Health Sciences (2019).
  17. Nielsen S S. “Total carbohydrate by phenol-sulfuric acid method”. In Food analysis laboratory manual. Springer, Cham (2017): 137-141.
  18. Nos P. “HiPer® Carbohydrates Estimation Teaching Kit (Quantitative); product code: HTBC003; number of experiments that can be performed: 10; duration of experiment”. DNSA method, 1.
  19. Piccolo A., et al. “A comparison of acid hydrolyses for the determination of carbohydrate content in soils”. Communications in Soil Science and Plant Analysis15-17 (1996): 2909-2915.
  20. Sadasivam S. “Biochemical methods”. New Age International (1996).
  21. Saravanan T S and Ravindranath M H. “Total free sugars, reducing sugars and glucose”. CMFRI Special Publication 7 (1981): 17-21.
  22. Somogyi M. “Determination of reducing sugars by Nelson-Somogyi method”. Journal of Biological Chemistry 200 (1952): 245.
  23. “Qualitative Tests Used For Carbohydrates Biology Essay” (2018).
  24. Yemm E W and Willis A. “The estimation of carbohydrates in plant extracts by anthrone”. Biochemical Journal3 (1954): 508-514.


Citation: Rathod Zalak R., et al. “Characterization of the Microbes Isolated from Salacia reticulata Raw Material and its Extract: A Simple Method to Reduce the Microbial Load in Herbal Products". Acta Scientific Pharmaceutical Sciences 6.3 (2022): 20-28.


Copyright: © 2022 Rathod Zalak R., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate30%
Acceptance to publication20-30 days

Indexed In

News and Events

  • Certification for Review
    Acta Scientific certifies the Editors/reviewers for their review done towards the assigned articles of the respective journals.
  • Submission Timeline for Upcoming Issue
    The last date for submission of articles for regular Issues is July 10, 2024.
  • Publication Certificate
    Authors will be issued a "Publication Certificate" as a mark of appreciation for publishing their work.
  • Best Article of the Issue
    The Editors will elect one Best Article after each issue release. The authors of this article will be provided with a certificate of "Best Article of the Issue"
  • Welcoming Article Submission
    Acta Scientific delightfully welcomes active researchers for submission of articles towards the upcoming issue of respective journals.

Contact US