Acta Scientific Pharmacology

Review Article Volume 3 Issue 12

Antidiabetic Effects of Hydroethanolic Rhizome Extract of Anchomanes Difformis (Araceae) in Streptozotocin-Induced Diabetic Rats

Chidi Chukwunenye

Clinical Laboratory Assistant at College of Medicine, University of Lagos, Nigeria

*Corresponding Author:Chidi Chukwunenye, Clinical Laboratory Assistant at College of Medicine, University of Lagos, Nigeria.

Received: October 07, 2022; Published: November 30, 2022

Abstract

Great efforts are ungoing in understanding and management of diabetes, the disease and disease related complications are increasingly unabated. In spite of the presence of known antidiabetic medicine in pharmaceutical market, remedies from medicinal plants are used with success to treat this disease. A. Difformis (Araceae) is a self- growing herbaceous plant that grows in the tropical forest and is very frequently cited as a medicinal plant used in the treatment of cough, diarrhea and diabetes in southwestern Nigeria. The aim of this study is to evaluate the hypoglycaemic effect of the hydro-ethanolic extract of the rhizome of A. Difformis.
The effect of A. Difformis (125-500 mg/kg) on blood glucose levels in normal and glucose loaded rats and STZ induced diabetic rats were determined. After 21days of sub-acute treatment, blood samples were collected from rats for lipid, biochemical and insulin profiling.
In normal rats, A. Difformis did not cause significant reduction in blood glucose. At a dose of 500mg/kg, a significant decrease in blood glucose levels was observed 60 min after glucose load. A. Difformis at 500 mg/kg administered acutely showed a signicant (p < 0.0001) decrease in blood glucose level in diabetic rats. Administered sub acutely, the extract at 500 mg/kg significantly (p < 0.0001) reduced glucose levels at day 7 with subsequent decrease at day 14 and 21.The extract did not show any significant reduction in fasting blood insulin levels and biochemical parameters when evaluated showed no significant change when compared to control.
The hypoglycaemic effects of A. difformis is by reduction of blood glucose levels through possibly combination of mechanisms. This pharmacological evaluation validate the claims and its use as an anti-diabetic medicinal plant in Nigeria.

Keywords: Antidiabetic; Hydroethanolic; Rhizome; Anchomanes Difformis; Streptozotocin

References

  1. Abraira C., et al. “Veterans affairs: cooperative study on glycaemic control and complications in type 2 diabetes. (VCSDM) results of the feasibility trials veterans affairs cooperative study in type 2 diabetes”. Diabetes Care 18 (1995): 1113-1123.
  2. Akhtar MA., et al. “Comparison of long-term anti hyperglycaemic and hypolipidaemic effects between Coccinia cordifolia (Linn.) and Catharanthus roseus (Linn) in alloxan-induced diabetic rats”. Research Journal of Medical Sciences 2 (2007): 29-34.
  3. Akinkurolere R., et al. “Laboratory evaluation of the toxic properties of forest Anchomanes, Anchomanes difformis against pulse beetle Callosobruchus maculates (Coleoptera: Bruchidae)”. Insect Science 1 (2006): 25-29.
  4. Anderson L., et al. “Enzyme immuneassay for intact human insulin in serum or plasma”. Clinical Chemistry 39 (1993): 578-582.
  5. Atawodi SE., et al.In vitro trypanocidal effect of methanolic extract of some Nigerian savannah plants (abstract)”. African Journal of Biotechnology 9 (2003): 317.
  6. Bansal R., et al. “Alloxan-glucose interaction: effect of incorporation of 14C-leucine into pancreatic islets of rats”. Acta Diabetologica Latina 17 (1980): 135-143.
  7. Bouquet A and Debray M. “Plantes médicinales de Côte-d’Ivoire”. Imprimerie Louis Jean, Paris (France) (1974): 232.
  8. Boylan JM., et al. “Differential regulation of multiple hepatic protein tyrosine phosphatases in alloxan diabetic rats”. Journal of Clinical Investigation 90 (1992): 174-179.
  9. Chukwurah J and Ajali U. “Antimicrobial investigationof the constituents of the methanolic extract of the rhizome of Anchomanes difformis”. Indian Journal. Pharmacopoeia. Sci 62 (2000): 296.
  10. Davenport HW. “Gastric mucosa haemorrhage in dogs, effect of acid aspirin and alcohol”. Gastroenterology 56 (1969): 439.
  11. Daziel JM. “Laxative effect of Ehretia cymose” (1937):
  12. De Sereday M., et al. “Prevalence of diabetes, obesity, hypertension and hyperlipidaemia in central area of Argentina”. Diabetes and Metabolism 30 (2004): 335-339.
  13. Dean PM and Matthews EK. “The bioelectrical properties of pancreatic islet cells: effect of diabetogenic agents”. Diabetologia 8 (1972): 173-178.
  14. Defronzo RA. “The triumvitae: cells, muscle, liver, A collusion responsible for NIDDM”. Lilly lecture 1987 (1988).
  15. Defronzo RA. “Pharmacologic therapy for type 2 diabetes mellitus”. Annals of Internal Medicine 131 (1999): 281-303.
  16. Dieye AM., et al. “Prospective study for the reduction and stabilization of glycaemia in diabetic rabbits by DIACODA, a substance derived from plant sources”. Post graduate qualification in Biotechnology. University of Cocody-Abidjan, UFR Biosciences, Laboratory of Biochemistry (2000): 1-36.
  17. Dunn JS., et al. “Necrosis of islets of Langerhans produced experimentally”. Lancet 1 (1943): 484-487.
  18. Eizirik DL., et al. “Major species differences between humans and rodents in the susceptibility to pancreatic beta-cell injury”. Proceedings of the National Academy of Sciences of the United States of America 91 (1994): 9253-9256.
  19. Ganda OP., et al. “Studies on streptozotocin induced diabetes”. Diabetes 25 (1976): 595-603.
  20. Gbéassor M., et al. “Antimalarial effects of eight African medicinal plants”. Journal of Ethnopharmacology 25 (1989): 115-118.
  21. Gbile ZO. “Ethnobotany, Taxonomy and Conservation of Medicinal Plants”. In: “the State of Medicinal Plants Research in Nigeria. Sofowora AE (ed.) University of Ibadan Press Nigeria (1986): 13-29.
  22. Gentilini M. “Tropical Medicine”. Editions Flammarion, Paris (1993): 928
  23. Glombitza KW., et al. “Hypoglycemic and antihyperglycemic effects of Zizyphus spinachristi in rats”. Planta Medica 60 (1994): 244-247.
  24. Goldberg DM and Spooner RJ. “Glutathione Reductase”. In: Methods in Enzymatic Analysis, VCH Weinhem, Germany (1983): 258-265.
  25. Gruppuso PA., et al. “Hepatic protein phosphotyrosine phosphatase. Dephosphorylation of insulin receptor and epidermal growth factor receptors in normal and alloxan diabetic rats”. Journal of Clinical Investigation 85 (1990): 1754-1760.
  26. Hundal RS., et al. “Mechanism by which metformine reduces glucose production in type 2 diabetes” (2000).
  27. Inzucchi SE., et al. “Eficacy and metabolic effect of metformine and troglitazone in type 2 diabetes mellitus”. The New England Journal of Medicine13 (1998): 867-872.
  28. Kadja B. “Evolution de quelques paramètres de diagnostique diabétique au cours d’un essai thérapeutique par un phytomédicament (DIACODA). Diplôme d’Etudes Approfondies de Biotechnologie”. Université de Cocody-Abidjan, UFR Biosciences, Laboratoire de Pharmacodynamie-Biochimique (1998): 31 .
  29. Kameswara Rao B., et al. “Herbal medicines: In the treatment of treatment of diabetes mellitus”. Manphar Vaidya Patrika (1997): 33-35
  30. Karthala, 75013 Paris 375.
  31. Katsumata K., et al. “Protective effect of diltiazem hydrochloride on the occurrence of alloxan- or streptozotocin-induced diabetes in rats”. Hormone and Metabolic Research 24 (1992): 508-510.
  32. Kerharo J and Bouquet A. “Plantes médicinales et toxiques de Côte-d’Ivoire, Haute-Volta”. Vigot et Frères, Editeurs. Paris (VIè) (1950):
  33. King EJ and Armstrong AR. “Determination of serum and bile phosphatase activity”. Canadian Medical Association Journal 31 (1934): 56-63.
  34. Koné MW. “Potentiel des plantes médicinales de Côte-d’Ivoire dans le contrôle des (2005).
  35. Mahabir D and Gulliford MC. “Use of medicinal plants for diabetes in Trinidad and Tobago”. Revista Panamericana de Salud Pública1 (1997): 1-16.
  36. Maiorana A. “Indigenous drugs in diabetes mellitus” (2001).
  37. NACOULMA O. “Plantes médicinales te pratiques médicales Naturelles, Université de Ouagadougou (Burkina-Faso)”. Bois et Forets des Tropiques (1996): 605.
  38. Neuwinger HD. “African Ethnobotany”. Poisons and drugs. Chemistry” (1996):
  39. Nishikawa T., et al. “The missing link: single unifying mechanism for diabetic complications”. Kidney International Supplements 77 (2000): S26-30.
  40. Odebiyi A and Sofowora AE. “Phytochemical Screening of Nigeria Medicinal Plants Part III”. Udoydia 41 (1990): 234-235.
  41. Oyetayo VO. “Comparative studies of the phytochemical and antimicrobial properties of the leaf, stem and tuber of Anchomanes difformis”. Pharmacology Toxicology4 (2007): 407-410.
  42. Pagila DE and Valentine WN. “Studies on the quantitative and qualitative characterization of erythrocyte glutathione peroxidase”. Journal of Laboratory and Clinical medicine 70 (1967): 158-169.
  43. Pousset JL. “Medicinal plants. Practical use”. Ed. Ellipse (1989): 150
  44. R Sy., et al. “Medicinal plants and the treatment of diabetes in Senegal: survey with patients”. Fundamental Clinical Pharmacology 22 (2007): 211-216.
  45. Reichard P., et al. The New England Journal of Medicine 329 (1993): 304.
  46. Rosmond ME., et al. “Instant proinsulin and β-cells function in lean and obese subjects with and without type 2 diabetes”. Diabetes Care4 (1999): 609-614.
  47. Sharma SB., et al. “Hypoglycaemic and hypolipidaemic effect of the ethanolic extract seeds of Eugenia jambolana in alloxan-induced diabetic rabbits”. Ethnopharmacology 85 (2003): 201-106.
  48. Shichiri M., et al. “Long term results of Kumamoto study on optimal diabetes control in type 2 diabetic patients”. Diabetes care2 (2000): B21-29.
  49. Shulman GI., et al. “Quantitation of muscle glycogen synthesis in normal subjects and subjects with non insulin dependent diabetes by 13C nuclear magnetic resonance spectroscopy”. The New England Journal of Medicine4 (1990): 223-228.
  50. Simonson DC., et al. “Mechanism of improvement in glucose metabolism after chronic glyburide therapy”. Diabetes 9 (1984): 838-845.
  51. Sofowora A. “Plantes médicinales et médecine traditionnelle d’Afrique”. Edition Karthala, 75013 Paris (1996):
  52. Srivastava Y., et al. “Antidiabetic and adaptogenic properties of Momordica charantia extract; an experimental and Clinical evaluation”. Phytotherapy Research 7 (1993): 285-289.
  53. Sumana G and Suryawarshi SA. “Effect of Vinca rosea extracts in treatment of streptozotocin diabetes in male albino rats”. Indian Journal of Experimental Biology 39 (2001): 748-758.
  54. Szkudelski T., et al. “Alloxan in vivo does not only exert deleterious effects on pancreatic B cells”. Physiology Research 47 (1998): 343-346.
  55. Tchiakpe I., et al. “Chemical and toxicological study of Anchomanesdifformis (England) ISHS”. Acta Horticulturae 96 (1996): 355-365.
  56. Viner R., et al. “Family stress and metabolic control in diabetes”. Archives Disease in Childhood5 (1996): 418-421.
  57. Walker AR and Silans R. “Les plantes utiles du Gabons Paul Lechevalier, Paris (1961): 19-132.
  58. Warram JH., et al. “Slow glucose removal rate and hyperinsulinemia precede the development of type 2 diabetes ion the offspring of diabetic patients”. Annals of Internal Medicine 12 (1990): 909-915.

Citation

Citation: Chidi Chukwunenye. “Antidiabetic Effects of Hydroethanolic Rhizome Extract of Anchomanes Difformis (Araceae) in Streptozotocin-Induced Diabetic Rats". Acta Scientific Pharmacology 3.12 (2022): 36-58.

Copyright

Copyright: © 2022 Chidi Chukwunenye. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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