Acta Scientific Pharmacology

Research Article Volume 3 Issue 4

Quantification of Chloroquine (Free Drug) in Liver Tissues by GC-MS

Ahmed MA Shihata* and Ahmed S Ahmed

Consultant Forensic Toxicology, Forensic Medicine Authority, Assuit, Egypt Chemical Lab, Egypt

*Corresponding Author: Ahmed MA Shihata, Consultant Forensic Toxicology, Forensic Medicine Authority, Assuit, Egypt Chemical Lab, Egypt.

Received: January 27, 2022; Published: February 17, 2022


Chloroquine (CQ) which belongs to the 4-aminoquinoline group of compounds and one of the most antimalarial. Chloroquine has been studied for the treatment and prevention of coronavirus disease 2019 (COVID-19), during treating coronavirus disease 2019 (COVID-19) some big side effects were noted. Quantification method involves a protein precipitation and liquid - liquid extraction procedure, analyses from alkaline and acidic extractions before GC/MS analysis and comparisons by two thermal program gas chromatography, first method thermal analyzing prefer than second method and alkaline is more quantified than acidic extraction.

Keywords: Chloroquine; Metabolites; Toxicity; GCMS


  1. Anthony C Moffat., et al. "Clarke's Analysis of Drugs and Poisons". Text book Pharmaceutical Press (2004).
  2. J E F Reynolds, Martindale, The Extra Pharmacopoeia, The Pharmaceutical Press, London, 30th edn, (1993).
  3. Basavaiah Kanakapura., et al. "Stability-indicating UV- spectrophotometric assay of chloroquine phosphate in pharmaceuticals". Research Journal of Pharmaceutical, Biological and Chemical Sciences (2016).
  4. Choudhary., et al. "Fatal suicidal poisoning by chloroquine in presence of alcohol-an interesting case report". Egyptian Journal of Forensic Sciences and Applied Toxicology 19.3 (2019).
  5. NV Rajeshkumar., et al. "Fatal toxicity of chloroquine or hydroxychloroquine with metformin in mice" (2020).
  6. Cassandra Doyno., et al. "Toxicity of chloroquine and hydroxychloroquine following therapeutic use or overdose". Clinical Toxicology1 (2021): 12-23.
  7. Hofmeister, F. “On the understanding of the effects of salts”. Naunyn-Schmiedeberg's Archives of Pharmacology 24 (1888): 247-260.
  8. Qiu., et al. "Toward an understanding of how the optical property of water- soluble cationic polythiophene derivative is altered by the addition of salts: The Hofmeister effect”. The Journal of Physical Chemistry C 117 (2013): 21870-21878.
  9. Krisna C Duong-Ly., et al. "Salting out of proteins using ammonium sulfate precipitation" (2014).


Citation: Ahmed MA Shihata and Ahmed S Ahmed. “Quantification of Chloroquine (Free Drug) in Liver Tissues by GC-MS". Acta Scientific Pharmacology 3.4 (2022): 16-19.


Copyright: © 2022 Ahmed MA Shihata and Ahmed S Ahmed. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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