Slamet Tuty*, Sri Ulfa Sulthan and Sri Maryam

Department of Pharmacy, Al-Ghifari University Bandung, Indonesia

*Corresponding Author: Slamet Tuty, Department of Pharmacy, Al-Ghifari University Bandung, Indonesia.

Received: October 29, 2021 Published: December 31, 2021

Abstract

Stone Parasite (Begonia sp.) has long been known by the people of Kabaena, Bombana Regency, Southeast Sulawesi, a plant known as a natural herb because of its ability to treat various diseases. People use this plant for the treatment of tumors and cancer. Stone Parasite contains secondary metabolites including saponins, tannins, flavonoids and polyphenols. Flavonoids are one of the largest natural phenolic group antioxidants found in all plants. This study aims to determine the levels of flavonoid compounds and test the antioxidant activity of ethanol extract of Stone Parasite using UV-Vis spectrophotometry. Extraction was carried out by maceration method using ethanol 96% with a yield of 14.8%. The results of the determination of the Stone Parasite water content was 2.6%. The determination of the total Flavonoid content of the ethanol extract of Stone Parasite was determined based on the absorbance value measured at a wavelength of 437 nm using a quercetin comparison. The results of the determination of total flavonoid levels were 16.68 mg QE/gr. The results of the antioxidant activity test on Stone parasite extract at a wavelength of 517 nm showed an IC₅₀ value of 6.7357µg/ml. Stone Parasit ethanol extract has a very strong antioxidant activity with the DPPH method 

Keywords: Stone Parasite; Total Flavonoids; Antioxidants; DPPH

References

1. Ritna A., et al. “Identification of flavonoid compounds in ethyl acetate fraction of benalu batu ( Begonia sp.)”. Originated from North 2.2 (2016).
2. Anam S., et al. “Citotoxic activity of Methanol Extract Stone Parasite (Begonia sp.): Ethnomedicine of Wana Tribe Central Sulawesi”. Journal of Pharmacy Indonesia1 (2014): 10-16.
3. Azizah ND., et al. “Determination Flavonoid level with AlCl method of Methanol Extract Skin Fruit Kakao (Theobroma cacao L.)”. 2.2 (2014): 45-49.
4. Ahmad AR., et al. “Determination of total Phenolic and Flavonoid level of Extract Methanol Fruit and Leaves of Patilaka (Etlingera elatior (Jack) R. M. SM)”. University Muslim Indonesia., Makasar. Pharmaceutical Sciences and Research1 (2015): 2407-2354.
5. Reynertsen K A., et al. “Antioxidant Potential of Seven Myrtaceous Fruits”. Ethnobotany Research and Applications 3 (2005): 025-035.
6. Winarsih H. “Natural Antioxidant and Free Radical”. Yogyakarta: Kanisius (2007).
7. Anam K., et al. “Isolation Triterpenoid compound of Red Alga (Eucheuma cottonii) Using Thin Layer Chromatography (TLC) and Analysis with Spectrophotometer UV-Vis and FTIR”. University Islam Maulana Malik Ibrahim Malang (2015).
8. Fajriyah N N., et al. “Parameter Test of Quality Standards Celery Herb Simplicia (Apium graveolens L.) From Pekalongan Regency”. Muhammadiyah University Purwokerto (2018).
9. Stankovic MS. “Total Phenolic Content, Flavonoid Concentration And Antioxidant Activity Of Marrubium Peregrinum L. Extracts”. Kragujevac Journal of Science 33 (2011): 63-72.
10. Irvan Ipandi., et al. “Determination of Total Flavonoid and Antioxidant Activity Ethanol Extract of Kajajahi Leaves (Leucosy kecapitellata Wedd.)”. Lambung Mangkurat University Banjarmasin., Journal Pharmascience1 (2016): 93-100.

Citation

Citation: Slamet Tuty., et al. “Determination of Total Flavonoid Levels and Antioxidant Activity from Ethanol Extracts of Stone Parasite (Begonia sp.) with DPPH Method (2,2-diphenyl-1-picrylhydrazyl)". Acta Scientific Pharmacology 3.1 (2022): 42-45.

Copyright

Copyright: © 2022 Slamet Tuty., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.