Acta Scientific Pharmacology

Research Article Volume 3 Issue 1

Determination of Total Flavonoid Levels and Antioxidant Activity from Ethanol Extracts of Stone Parasite (Begonia sp.) with DPPH Method (2,2-diphenyl-1-picrylhydrazyl)

Slamet Tuty*, Sri Ulfa Sulthan and Sri Maryam

Department of Pharmacy, Al-Ghifari University Bandung, Indonesia

*Corresponding Author: Slamet Tuty, Department of Pharmacy, Al-Ghifari University Bandung, Indonesia.

Received: October 29, 2021 Published: December 31, 2021


Stone Parasite (Begonia sp.) has long been known by the people of Kabaena, Bombana Regency, Southeast Sulawesi, a plant known as a natural herb because of its ability to treat various diseases. People use this plant for the treatment of tumors and cancer. Stone Parasite contains secondary metabolites including saponins, tannins, flavonoids and polyphenols. Flavonoids are one of the largest natural phenolic group antioxidants found in all plants. This study aims to determine the levels of flavonoid compounds and test the antioxidant activity of ethanol extract of Stone Parasite using UV-Vis spectrophotometry. Extraction was carried out by maceration method using ethanol 96% with a yield of 14.8%. The results of the determination of the Stone Parasite water content was 2.6%. The determination of the total Flavonoid content of the ethanol extract of Stone Parasite was determined based on the absorbance value measured at a wavelength of 437 nm using a quercetin comparison. The results of the determination of total flavonoid levels were 16.68 mg QE/gr. The results of the antioxidant activity test on Stone parasite extract at a wavelength of 517 nm showed an IC50 value of 6.7357µg/ml. Stone Parasit ethanol extract has a very strong antioxidant activity with the DPPH method 

Keywords: Stone Parasite; Total Flavonoids; Antioxidants; DPPH


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Citation: Slamet Tuty., et al. “Determination of Total Flavonoid Levels and Antioxidant Activity from Ethanol Extracts of Stone Parasite (Begonia sp.) with DPPH Method (2,2-diphenyl-1-picrylhydrazyl)". Acta Scientific Pharmacology 3.1 (2022): 42-45.


Copyright: © 2022 Slamet Tuty., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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