Evaluation of Anti-Inflammatory Effect of Combination Therapy of Silymarin Nanomicelles plus Berberine Nanomicelles in LPS-induced Depressive-like Behavior in Mice
Aysan Hasanitabar1, Marjan Fatholahi1, Ali Bitaraf2, Seyed Mahdi Rezayat1,3 and Seyyedeh Elaheh Mousavi1*
1Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran
2School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran
3Department of Medical Nanotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran
*Corresponding Author: Seyyedeh Elaheh Mousavi, Department of Pharmacology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
July 08, 2021; Published: November 25, 2021
Depression is one of the most common mental illnesses globally, and unfortunately, current medications as a treatment, in this case, have a wide range of side effects. Therefore, finding new drugs is a valuable step in the treatment of depressive disorders. Berberine and Silymarin have many biological therapeutic properties like antioxidant, anti-inflammatory, and anti-cancer, while their bioavailability is not strong.
In order to increase the effectiveness and improve the bioavailability of drugs, new tools like nano micelles are suggested. Therefore, we aimed to investigate the effect of Berberine and Silymarin nanomicelles (NB+ NS) on the model of depression induced by Lipopolysaccharides (LPS) in mice.
LPS (1 mg/kg, i.p.) was injected to induce depression-like behavior, then, 24 h later, the treatment began, and behavioral tests like forced swimming test (FST), open field test (OFT), and tail suspension test (TST) were carried out. Also, the brain level of Tumor Necrosis Factor- α (TNF-α) and Interleukin-1 Beta (IL-1ß) were measured as well. In this study, the mice were divided into different groups including, control, LPS, Nanomicelle, Fluoxetine, Berberine, Silymarin, and NB 5+ NS 25.
All data were analyzed by Graph pad prism (version 5), and SPSS (P < 0.05 was considered significant).
This study showed that FST immobility time, TST immobilization time, brain levels of TNF-α and IL-1ß significantly decreased in NS 25+ NB 5 and fluoxetine groups compared to LPS and control groups (P < 0.05).
Keywords: Nanomicelles; Silymarin; Berberin; LPS; Depression
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