Acta Scientific Pharmacology

Research Article Volume 1 Issue 6

Derivative Spectrophotometric Method for Determination of Lamotrigine and Risperidone in Pharmaceutical Dosage Forms

MK Prashanth1, TD Devaraj1, BK Jayanna1* and KP Roopa2

1Department of Chemistry, B. N. M. Institute of Technology, Bengaluru, India
2Department of Chemistry, Sapthagiri College of engineering, Bengaluru, India

*Corresponding Author: BK Jayanna, Professor and Head, Department of Chemistry, B. N. M. Institute of Technology, Bengaluru, India.

Received: March 28, 2020; Published: May 31, 2020



This work proposes a method for simple spectrophotometric determination of Lamotrigine (LMT) in presence of Risperidone (RSP) was accomplished using second derivative spectrophotometry. In the present method, neither a chromogenic reagent nor any other reagents are required. Excipients with drug molecule do not interfere with the determination. The Lamotrigine can be determined in the range of 1.0 -20 μg/mL. All other analytical parameters are established. The proposed method was more appropriate to the pharmaceutical formulation for the determination of Lamotrigine.

Keywords: Lamotrigine; Risperidone; Second Derivative Spectrophotometry



  1. Sean C Sweetman and Martindale. “The Complete Drug Reference”. 34th edition. Pharmaceutical Press, London (2005).
  2. Dreassi E., et al. “Quantitative analysis of lamotrigine in plasma and tablets by planar chromatography and comparison with liquid chromatography and UV spectrophotometry”. Journal of AOAC International 77 (1996): 1277-1280.
  3. Patil KM and Bodhankar SL. “High-performance thin-layer chromatographic determination of lamotrigine in serum”. Journal of Chromatography B 823 (2005): 152-157.
  4. Guitton S., et al. “Determination of lamotrigine and its metabolites in human plasma by liquid chromatography-mass spectrometry”. Rapid Communications in Mass Spectrometry 831 (2006): 603-607.
  5. Dasgupta A and Hart AP. “Lamotrigine analysis in plasma by gas chromatography–mass spectrometry after conversion to a tertbutyldimethylsilyl derivative”. Journal of Chromatography B 693 (1997): 101-107.
  6. Shihabi ZK and Oles KS. “Serum lamotrigine analysis by capillary electrophoresis”. Journal of Chromatography B: Biomedical Sciences and Applications 157 (1996): 119-123.
  7. Biddlecombe RA., et al. “Validation of a radioimmunoassay for the determination of human plasma concentrations of lamotrigine”. Journal of Pharmaceutical and Biomedical Analysis 8 (1990): 691-694.
  8. Calvo MEB., et al. “Determination of lamotrigine by adsorptive stripping voltammetry using silver nanoparticle-modified carbon screen-printed electrodes”. Talanta 74 (2007): 59-64.
  9. Gholivand MB., et al. “Determination of lamotrigine by using molecularly imprinted polymer–carbon paste electrode”. Journal of Electroanalytical Chemistry 692 (2013): 9-16.
  10. Fernando ML and Marlene AS. “Simultaneous plasma lamotrigine analysis with carbamazepine, carbamazepine 10, 11 epoxide, primidone, phenytoin, phenobarbital, and PEMA by micellar electrokinetic capillary”. Chromatographic Journal of Analytical Toxicology 27 (2003): 604-613.
  11. Ghatol S., et al. “Liquid chromatography-tandem mass spectrometry method for the estimation of lamotrigine in human plasma: Application to a pharmacokinetic study”. Journal of Pharmaceutical Analysis 3 (2013): 75-83.
  12. Rajendraprasad N., et al. “Sensitive spectrophotometric determination of lamotrigine in bulk drug and pharmaceutical formulations using bromocresol green”. Eclética Química Journal 35 (2010): 55-67.
  13. Rajendraprasad N., et al. “Simple and sensitive spectrophotmetric determination of lamotrigine in pure form and in dosage forms”. Pharmaceutica Analytica Acta 3 (2012): 188-192. 
  14. Fadhil MN and Muhammad SM. “Four spectrophotometric methods for simultaneous determination of carbamazepine and lamotrigine in binary mixtures and urine samples”. The Malaysian Journal - Analytical Sciences 18 (2014): 491-506.
  15. Christine J., et al. “Differential pulse polarographic studies of risperidone in pharmaceutical formulations”. Croatica Chemica Acta 79 (2006): 541-544.
  16. Zhenghua S and Changna W. “Sensitive chemiluminescence assay for risperidone in pharmaceutical preparations”. Journal of Pharmaceutical and Biomedical Analysis 36 (2004): 491-494.
  17. Yu-guan WEN., et al. “Determination of risperidone in human plasma with LC-ESI-MS/MS”. Strait Pharmaceutical Journal (2007).
  18. Danel C., et al. “Analytical and semipreparative enantioresolution of 9-hydroxyrisperidone main metabolite of risperidone using high-performance liquid chromatography and capillary zone electrophoresis. Validation and determination of enantiomeric purity”. Journal of Chromatography A 1163 (2007): 228-236.
  19. Cabovska B., et al. “Determination of risperidone and enantiomers of 9-hydroxyrisperidone in plasma by LC-MS/MS”. Journal of Chromatography B 852 (2007): 497-504.
  20. Bhatt J., et al. “Liquid chromatography/tandem mass spectrometry method for simultaneous determination of risperidone and its active metabolite 9‐hydroxyrisperidone in human plasma”. Rapid Communications in Mass Spectrometry 20 (2006): 2109-2114.
  21. Le Moing JP., et al. “Determination of risperidone and 9-hydroxyrisperidone in human plasma by high-performance liquid chromatography with electrochemical detection”. Journal of Chromatography B 614 (1993): 333-339.
  22. Locatelli I., et al. “Simultaneous determination of risperidone and 9-hydroxyrisperidone enantiomers in human blood plasma by liquid chromatography with electrochemical detection”. Journal of Pharmaceutical and Biomedical Analysis 50 (2009): 905-910.
  23. Avenoso A., et al. “Determination of risperidone and its major metabolite 9-hydroxyrisperidone in human plasma by reversed-phase liquid chromatography with ultraviolet detection”. Journal of Chromatography B 746 (2000): 173-181.
  24. Schatz DS and Saria A. “Simultaneous Determination of Paroxetine, Risperidone, and 9-Hydroxyrisperidone in Human Plasma by High-Performance Liquid Chromatography with Coulometric Detection”. Pharmacology 60 (2000): 51-56.
  25. Narayana B and Divya NS. “Development and Validation of Spectrophotomtric Method for the, Determination of Risperidone”. Der Pharmacia Lettre 3 (2011): 104-109.
  26. Kumar AP., et al. “Simultaneous second-derivative spectrophotometric determination of cobalt and vanadium using 2-hydroxy-3-methoxy benzaldehyde thiosemicarbazone”. Journal of Analytical Chemistry 63 (2008): 26-29. 
  27. Bhalodia HA., et al. “Application of absorbance ratio method for simultaneous spectroscopic estimation of rabeprazole sodium and levosulpiride in combined pharmaceutical formulation”. Inventi Rapid: Pharma Analysis and Quality Assurance (2012).
  28. Reddy V., et al. “First Order Derivative Spectrophotometric Determination of Vanadium(V) and Iron(III) Individually and Simultaneously”. Journal of Analytical Chemistry 58 (2003): 442-446.
  29. Bosch Ojeda C and Sanchez RF. “Recent applications in derivative ultraviolet/visible absorption spectrophotometry: 2009-2011: A review”. The Microchemical Journal 106 (2013): 1-16.


Citation: BK Jayanna., et al. "Derivative Spectrophotometric Method for Determination of Lamotrigine and Risperidone in Pharmaceutical Dosage Forms".Acta Scientific Pharmacology 1.6 (2020): 11-16.


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