Solenostemon rotundifolius leaves have widely been used in ethnomedicine for the management of diarrhea and other diseases of the gastrointestinal tract. In this study, the spasmolytic effects of its leaf extract and fractions on normal and acetylcholine induced rhythmic contractions of an isolated rabbit jejunum were investigated to possibly validate this traditionally acclaimed anti- diarrheal property. Crude extract was prepared from the plant’s leaves and 7 fractions were obtained following fractionation of this crude extract. About 2-3 cm length of jejunum isolated from a stunned rabbit was suspended vertically in a 30 ml organ bath containing tyrode solution and bubbled with air. After equilibration, various doses of the extract, fractions and atropine were separately administered to the piece of tissue to determine their effects. Effects of graded doses of acetylcholine on the piece of tissue were also determined and repeated separately in the presence of atropine and the test extract and fractions with adequate washing preceding each administration. Results obtained showed that the effect of the crude extract on the piece of isolated tissue was inhibitory as graded doses like atropine, produced a dose dependent relaxation of the tissue, lowering the amplitude of contractions in each case with 0.33 mg/ml producing 71.42 percent inhibitory effect and 1.00 mg/ml producing an inhibitory activity of 91.67 percent. The effects of all the fractions were also inhibitory but fraction 5 produced the highest relaxation effect. Acetylcholine when applied caused dose dependent contractions which were significantly inhibited by the crude extract and fractions in a manner which compared favourably with that of atropine. The order of strengths of inhibitions of the test agents on acetylcholine induced contractions is: F5>F4>F3>CRUDE>F6>F1>F2>F7. We therefore conclude that Solenostemon rotundifolius leaf extract and fractions, having shown significant spasmolytic effects on an isolated piece of jejunum, may contain active components with anti-diarrheal potentials and be of value in the management of the disease.

Keywords:Acetylcholine; Fraction; Inhibitions; Tissue; Solenostemon rotundifolius; Spasmolytic

References

1. Orhue ES., et al. “Haematological and histopathological studies of Jatropha tanjorensis leaves in Rabbits”. Asian Journal of Biological Sciences 1.2 (2008): 84-89.
2. Akomas SC., et al. “In vivo and in vitro Spasmolytic effect of Ficus sur Forssk ethanol leaf extract on the gastrointestinal tract”. British Biotechnology Journal 4.11 (2014): 1182-1190.
3. Belanus MJ and Kinghorn AD. “Drug discovery from medicinal plants”. Life Sciences 78.5 (2005): 431-441.
4. Ijioma SN., et al. “Uterine relaxation potential of Moriaga oleifera via the Muscarinic receptor pathway”. British Journal of Pharmaceutical Research 4.20 (2014): 2455-2462.
5. Venkateshappa SM and Sreenath KP. “Potential medicinal plants of lamiaceae”. American International Journal of Research in Formal, Applied and Natural Sciences 3.1 (2013): 82-87.
6. Enyiukwu DN., et al. “Potentials of Huasa potato (Solonesteon rotundifolius Poir.) J.K. Morton and Management of its tuber Rot in Nigeria”. Greener Journal of Agronomy, Forestry and Horticulture 2.2 (2014): 027-037.
7. Sugri I., et al. “Sustaining Frafra Potato (Solenostemon rotundifolius Poir.) in the Food Chain; Current Opportunities in Ghana”. Journal of Plant Sciences 1.4 (2013): 68-75.
8. Anbuselvi S and Priya MH. “Nutritional and anti nutritional constituents of Plectranthus rotundifolius”. International Journal of Pharmaceutical Sciences Review and Research 22.1 (2013): 213-215.
9. Abbot D and Andrew RS.” An introduction to chromatography”. 2nded. Longman press London (1970): 72-78.
10. Eglen RM. “Muscarinic receptors and gastrointestinal tract smooth muscle functions”. Life Science 68 (2001): 2573-2578.
11. Ehlert FJ. “Contractile role of M2 and M3 in gastrointestinal, airway and urinary bladder smooth muscles”. Life Sciences 74.2-3 (2003): 355-366.
12. Uchendu CN. “Role of Ca2+ on the uterine force stimulated by glycoside from the root of Dalbergia saxatilis”. Indian Journal of Physiology and Pharmacology 43.2 (1999): 171-178.
13. Venkataranganna MV., et al. “Antispasmodic activity of SJ-200 (Himcospaz) an herbalpreparation”. Pharmaceutical Biology 40.6 (2002): 416-421.
14. Jespersen B., et al. “Measurement of Smooth Muscle Function in the Isolated Tissue Bath-applications to Pharmacology Research”. Journal of Visualized Experiment 95 (2015): 52324.
15. Hasler WL. “Pharmacotherapy for intestinal motor and sensory disorders”. Gastroenterology Clinics North America 32 (2003): 707-732.
16. Harbor CI., et al. “Nautraceutical and elemental composition of Solenostemon rotundifolius leaves grown in Umudike Nigeria”. The Nigerian Agricultural Journal 47.2 (2016): 77-84.
17. Zorofchian MS., et al. “Loranthus micranthus Linn: Biological activity and phytochemistry”. Evid Based Complement of Alternative Medicine (2013): 273712. 
18. Hejazian SH., et al. “Spasmolytic and anti-spasmodic action of Trachyspermum ammi essence on rat’s ileum contraction”. North American Journal of Medical Sciences 612 (2014): 643-647.
19. Madubuike KG., et al. “Anticholinergic Activity of Loranthus micranthus (Linn) Leaves Extract on Gastrointestinal Smooth Muscles”. Comprehensive Journal of Medical Science 3.1(2015): 20-27. 
20. Ijioma SN., et al. “Uterine relaxation potential of Moriaga oleifera via the Muscarinic receptor pathway”. British Journal of Pharmaceutical Research 4.2 (2014): 2455-2462.
21. Akomas SC., et al. “In vivo and in vitro Spasmolytic effect of Ficus sur Forssk ethanol leaf extract on the gastrointestinal tract”. British Journal of Biotechnology 4.11 (2014): 1182-1190.

Citation

Citation: Ijioma SN., et al. “Muscarinic Receptor Mediated Spasmolytic Effects of Crude Extract and Fractions Prepared from Solenostemon rotundifolius Leaves on an Isolated Rabbit Jejunum”Acta Scientific Pharmacology 1.1 (2020): 02-10.

Copyright

Copyright: © 2020 Ijioma SN., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.