Priyodarshi Sengupta*, Nirmal Polle, Biplabendu Talukdar and Niranjan Bhattacharya

Department of Regenerative Medicine and Translational Science, School of Tropical Medicine, Kolkata, India

*Corresponding Author: Priyodarshi Sengupta, Department of Regenerative Medicine and Translational Science, School of Tropical Medicine, Kolkata, India.

Received: September 08, 2025; Published: November 30, 2025

Abstract

Introduction: Freshly collected amniotic membrane have been successfully used for treating diabetic foot ulcers (DFUs) since 1999. Freshly collected amniotic membrane is supposed to contain an array of growth factors, cytokines, and different types of potent cells that can participate in wound healing mechanism and act as a novel cell therapy model.

Method: Patients with confirmed diagnosis of diabetes, on oral anti-diabetic medication and insulin therapy presented with non-healing ulcers were enrolled in the study following the inclusion and exclusion criteria. Out of 9 patients, 4 patients (mean age 51 yrs, all male) were selected for the study. Human amniotic membrane was collected from healthy mothers undergoing caesarean section after their informed. Donor screening for ABO Rh blood grouping, and serological tests for HIV-I/II, Hepatitis B & C, VDRL, CMV and Syphilis were carried out before the collection of the amniotic membrane. After collection, the amniotic membranes were irrigated thoroughly with normal saline and any blood clots were removed manually. Screening of the patients included blood tests and checking for HIV-I/II, Hepatitis B & C, VDRL, CMV, malaria and toxoplasmosis. At baseline visit, all patients provided their informed consent. The wounds were thoroughly washed with normal saline, followed by the application of freshly collected human amniotic membrane (amnion or chorionic part). The amnion side was applied in ulcers where epithelialisation was required and the chorion side where vascularization was needed. Patient visit was scheduled after every 5 to 6 days and the interval between each visit increased after the first couple of months to 8 to 9 days approximately.

Results: No graft rejection was observed in any of the four patients in any of the visits. In the later part of the study, the duration of next patient visit was intentionally increased to observe any rejection or adverse events in patients. Patient follow up was successfully extended from 5-6 to 8-9 days approximately. At each visit, the wound size was measured which showed epithelialization, appearance of granulation tissue and reduction in the overall wound size. The wound region in all the four patients showed less discharge, lowering of pain, local oedema and induration. No adverse events like fever, inflammation, discharge or rejection were reported.

Conclusion: Freshly collected amniotic membrane can be an effective economical, biological dressing model for the treatment of diabetic non-healing ulcers demonstrating favourable clinical outcomes and good patient tolerance.

Keywords:Diabetic Foot Ulcer (DFU); Freshly Collected Human Amniotic Membrane

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Citation

Citation: Priyodarshi Sengupta., et al. “Freshly Collected Amniotic Membrane as a Novel Cell Therapy for Treating Diabetic Non-Healing Ulcers".Acta Scientific Neurology 8.12 (2025): 57-69.

Copyright

Copyright: © 2025 Priyodarshi Sengupta., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.