Acta Scientific Neurology (ASNE) (ISSN: 2582-1121)

Research Article Volume 5 Issue 5

The Effect of L-NAME on the Spectrum of Amino Acids and Biogenic Amines in Hippocampus and Brain Cortex During Subtotal Cerebral Ischemia

Razvodovsky YE2*, Smirnov VY1, Doroshenko EM1, PereverzevVA3 and Maksimovich NYe1

1Grodno State Medical University, Republic of Belarus, Grodno, Str. Gorky
2Institute Biochemistry of Biologically Active Substances Academy of Science of Belarus, Republic of Belarus, Grodno, Str. Boulevard of Lenin’s komsomol
3Belarusian State Medical University, Dzerzhinskogo Av., Minsk, Republic of Belarus

*Corresponding Author:Razvodovsky YE, Institute Biochemistry of Biologically Active Substances Academy of Science of Belarus, Republic of Belarus, Grodno, Str. Boulevard of Lenin’s Komsomol.

Received: March 19, 2022; Published: March 25, 2022

Abstract

The Aim of this Study: The aim of this study was to estimate the changes in the pool of free amino acids and biogenic amines in hippocampus and brain cortex of rats undergoing subtotal cerebral ischemia (SCI) and treated with L-NAME.

Methods: The experiments were carried out on 18 white outbred female rats (6 animals in each group), weighing 180 - 220g. Subtotal cerebral ischemia (SCI) was simulated in the rats of the experimental groups by ligation of both carotid arteries for one hour. L-NAME was injected intravenously at a dose of 5 mg/kg immediately before ligation of the common carotid artery. The spectrum of compounds determined included proteinogenic amino acids, ornithine, citrulline, a number of related compounds (taurine, α-aminobutyrate, etc.), and biogenic amines. The analysis was carried out on an Agilent 1100 chromatograph by reverse phase chromatography with precolumn derivatization with o-phthalic aldehyde and 3-mercaptopropionic acid in Na-borate buffer.

Results: Subtotal cerebral ischemia induced imbalance in the pool of amino acids and their derivates in hippocampus and brain cortex. The effect of SCI on the pool of free AA and neuroactive compounds in the studied sections of the rats brain is basically the same, however, there is a group of compounds, including aspartate, ornithine, and GABA, for which the nature of the effect is specific for the hippocampus and the brain cortex. Preventive administration of L-NAME partially prevents this imbalance; however induce the changes in the levels of bran chain amino acids (BCAA), arginine, gistidine, treonine and taurine.

Keywords: COVID- 19; SARS Virus; Inflammatory Mediators; Central Nervous System

References

  1. Smirnov VYu., et al. “Influence of the composition of amino acids with a branched hydrocarbon chain, tryptophan and taurine on the exchange of amino acids in experimental models of alcoholism”. Ukrainian Biochemical Journal 4 (2003): 101-107.
  2. Kanunnikova NP., et al. “Changes in the metabolism of GABA, glutamate and other neuroactive amino acids at different times of experimental cerebral ischemia”. Bulletin of the GrSU 3 (2006): 102-106.
  3. Razvodovsky YE., et al. “The content of amino acids and their derivatives in the cerebral cortex of rats with its partial ischemia”. Bulletin of the Smolensk State Medical Academy 1 (2019): 5-9.
  4. Razvodovsky YE., et al. “Levels of Free Amino Acids and their Derivatives in the Brain Cortex of Rats during Unilateral Ischemia”. International Journal Neuroscience and Behavior 1 (2017): 18-21.
  5. Maksimovich NYe. “Tolerance of hypoxic hypoxia in rats with cerebral ischemia treated by NO-synthase modulators”. Hypoxia Medical 1-2 (2004): 20-23.
  6. Razvodovsky YE. “Alcohol attributable fraction of stroke mortality in Russia”. Journal of the Neurological Science 1 (2013): 231.
  7. Ryosuke MD. “Effect of danttrolene on extracellular glutamate concentration and neuronal death in the rat hippocampal CA1 region subjected to transient ischemia”. Anesthesiology 96 (2002): 705-710.
  8. Salter M., et al. “Substantial regional and hemispheric differences in brain nitric oxide synthase (NOS) inhibition following intracerebroventricular administration of N-nitro-L-arginine (L-NA)and its methyl ester (L-NAME)”. Neuropharmacology 34 (1995): 639-649.
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Citation

Citation: Razvodovsky YE., et al. “The Effect of L-NAME on the Spectrum of Amino Acids and Biogenic Amines in Hippocampus and Brain Cortex During Subtotal Cerebral Ischemia". Acta Acta Scientific Neurology 5.5 (2022): 03-08.

Copyright

Copyright: © 2022 Razvodovsky YE., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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