Abstract

Introduction: Stroke is among the leading causes of disability and mortality in many countries of the world. The pathogenic mechanisms of ischemic stroke are very complex and have not been completely understood.

Objective: The aim of this study was to estimate the amino acid (AA) pool in the brain hemisphere cortex of rats during subtotal cerebral ischemia (SCI).

Methods: The changes in the pool of AAs and their derivatives in the frontal cortex of the cerebral hemispheres after 2-hour bilateral filament occlusion were studied in 18 rats. The analyses of the levels of AAs and their derivatives were carried out in the supernatant of protein-free tissue homogenates by reversed-phase liquid chromatography using an Agilent 1100 chromatograph.

Results: The results showed that the concentration of several AAs was altered after 2 hours of SCI. There were some specific features of AA imbalance in the left and right hemispheres. In particular, in the left hemisphere, we detected a reduction in the levels of glutamate, threonine, taurine, tyrosine, tryptophan and α-aminoadipine acid, as well as an increase in the level of ornithine. In the right hemisphere, a decrease in the levels of asparagine, serine and phenylalanine was found.

Conclusion: The results of the present study indicate that SCI induced an imbalance in the AA pool in the brain hemisphere cortex of rats. The specific features of the changes in the pool of AAs in the left and right hemispheres bear witness to asymmetry of the AA imbalance during SCI.

Keywords: Amino Acids; Brain Hemispheres; Subtotal Ischemia

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Citation

Citation: Razvodovsky YE., et al. “Interhemispheric Asymmetry in the Pool of Amino Acids during Subtotal Cerebral Ischemia". Acta Scientific Neurology 3.3 (2020): 03-07.

Copyright

Copyright: © 2020 Razvodovsky YE., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.