Ishaq Hossion¹, Md Shahin Mahmud¹, Md Aoulad Hosen¹, Md Saiduzzaman², Fatema Tuj Zohora¹, Md Khaled Hossain¹, Md Shajedur Rahman³ and Nazmi Ara Rumi¹*

¹Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

*Corresponding Author: Nazmi Ara Rumi, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

Received: January 10, 2023; Published: January 31, 2023

Abstract

Postoperative wound infections in surgical patients after surgeries, as well as the emergence and spread of drug-resistant strains, have been identified as a key challenge in the medical field and also can cause mortality, morbidity and economic burden. Therefore, this study investigated some common aerobic pathogenic isolates and their antibiotic sensitivity pattern in patients with post-surgical wound infections in a territory hospital in Dinajpur, Bangladesh. Bacteriological analysis was performed on wound swab samples collected from 15 patients with a medical assessment of post-surgical wound infections at Dinajpur Medical College. Wound swabs were collected from different surgical wound during dressing and inoculated on Nutrient agar, MacConkey agar, EMB agar, Blood agar, Cetrimide agar, and Mannitol Salt agar for isolation and identification of Escherichia coli, Pseudomonas spp. and Staphylococcus spp. The modified Kirby-Bauer disk diffusion technique was utilized for antibiotic sensitivity tests. Out of 15 samples, 10 (66.67%) samples were positive, of which 4 were male and 6 were female patients with age ranged from 15- ≥50 years. A high predominance of aerobic bacteria was observed and Staphylococcus spp. (42.11%), E. coli (31.58%) and Pseudomonas spp. (26.31%) were predominantly identified. The highest number of the positive case found in the age group ≥50 (40%) and the lowest positive cases were found in the age group 15-30 (10%) respectively. Among 19 bacterial isolates, higher growth of isolates (52.63%) were found in the age above ≥50, whereas the lowest growth of isolates was found in the age group 15-30 (5.27%) respectively. Sequencing the 16S rRNA gene, E. coli was identified with a 1466 bp PCR band. Gram-positive bacteria were susceptible to Cloxacillin 7/8 (87.5%) and Gentamicin 6/8 (75%), whereas, Gram-negative isolates were most effective to Ofloxacin, Ciprofloxacin, Gentamicin and Amikacin 5/5 (100%). Hence, the most predominant bacteria in post-operative wound infection were Staphylococcus spp. and E. coli. The antibiotic sensitivity tests revealed that numerous and multi-drug resistant bacteria are involved in post-operative wound infection. Therefore, routine microbiological investigation of wound specimens and antibiotic susceptibility testing will be advised by the clinicians in treating wound infections.

Keywords: Aerobic Bacteria; Drug-Resistant; Post-Operative Wound Infections; Wound Swabs

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Citation

Citation: Nazmi Ara Rumi., et al. “Determination of Pathogenic Bacteria Isolated from Post-Operative Wound Infections and Their Antibiotic Resistance Pattern: A Hospital-Based Cross-Sectional Study". Acta Scientific Medical Sciences 7.2 (2023): 177-185.

Copyright

Copyright: © 2022 Nazmi Ara Rumi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.