Acta Scientific Medical Sciences (ASMS)(ISSN: 2582-0931)

Research Article Volume 7 Issue 2

Determination of Pathogenic Bacteria Isolated from Post-Operative Wound Infections and Their Antibiotic Resistance Pattern: A Hospital-Based Cross-Sectional Study

Ishaq Hossion1, Md Shahin Mahmud1, Md Aoulad Hosen1, Md Saiduzzaman2, Fatema Tuj Zohora1, Md Khaled Hossain1, Md Shajedur Rahman3 and Nazmi Ara Rumi1*

1Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

2Stamford University, Dhaka, Bangladesh

3Department of Medicine, Surgery and Obstetrics, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh

*Corresponding Author: Nazmi Ara Rumi, Department of Microbiology, Hajee Mohammad Danesh Science and Technology University, Dinajpur, Bangladesh.

Received: January 10, 2023; Published: January 31, 2023

Abstract

Postoperative wound infections in surgical patients after surgeries, as well as the emergence and spread of drug-resistant strains, have been identified as a key challenge in the medical field and also can cause mortality, morbidity and economic burden. Therefore, this study investigated some common aerobic pathogenic isolates and their antibiotic sensitivity pattern in patients with post-surgical wound infections in a territory hospital in Dinajpur, Bangladesh. Bacteriological analysis was performed on wound swab samples collected from 15 patients with a medical assessment of post-surgical wound infections at Dinajpur Medical College. Wound swabs were collected from different surgical wound during dressing and inoculated on Nutrient agar, MacConkey agar, EMB agar, Blood agar, Cetrimide agar, and Mannitol Salt agar for isolation and identification of Escherichia coli, Pseudomonas spp. and Staphylococcus spp. The modified Kirby-Bauer disk diffusion technique was utilized for antibiotic sensitivity tests. Out of 15 samples, 10 (66.67%) samples were positive, of which 4 were male and 6 were female patients with age ranged from 15- ≥50 years. A high predominance of aerobic bacteria was observed and Staphylococcus spp. (42.11%), E. coli (31.58%) and Pseudomonas spp. (26.31%) were predominantly identified. The highest number of the positive case found in the age group ≥50 (40%) and the lowest positive cases were found in the age group 15-30 (10%) respectively. Among 19 bacterial isolates, higher growth of isolates (52.63%) were found in the age above ≥50, whereas the lowest growth of isolates was found in the age group 15-30 (5.27%) respectively. Sequencing the 16S rRNA gene, E. coli was identified with a 1466 bp PCR band. Gram-positive bacteria were susceptible to Cloxacillin 7/8 (87.5%) and Gentamicin 6/8 (75%), whereas, Gram-negative isolates were most effective to Ofloxacin, Ciprofloxacin, Gentamicin and Amikacin 5/5 (100%). Hence, the most predominant bacteria in post-operative wound infection were Staphylococcus spp. and E. coli. The antibiotic sensitivity tests revealed that numerous and multi-drug resistant bacteria are involved in post-operative wound infection. Therefore, routine microbiological investigation of wound specimens and antibiotic susceptibility testing will be advised by the clinicians in treating wound infections.

Keywords: Aerobic Bacteria; Drug-Resistant; Post-Operative Wound Infections; Wound Swabs

References

  1. Bailey H., et al. “Bailey and Love's short practice of surgery”. CRC Press (2008).
  2. Pradhan GB., et al. "Comparative study of post-operative wound infection following emergency lower segment caesarean section with and without the topical use of fusidic acid”. Nepal Medical College Journal3 (2009): 189-191.
  3. Ramesh A., et al. “Surgical site infections in a teaching hospital. Clinico Microbiological and Epidemiological profile”. International Journal of Biology and Medical Research3 (2012): 2050-2053.
  4. Mangram AJ., et al. “Hospital Infection Control Practices Advisory Committee. Guideline for prevention of surgical site infection, 1999”. Infection Control and Hospital Epidemiology4 (1999): 247-280.
  5. Horan TC., et al. “CDC definitions of nosocomial surgical site infections, 1992: a modification of CDC definitions of surgical wound infections”. Infection Control & Hospital Epidemiology10 (1992): 606-608.
  6. Weigelt JA., et al. “Surgical site infections: causative pathogens and associated outcomes”. American Journal of Infection Control2 (2010): 112-120.
  7. Coello R., et al. “Adverse impact of surgical site infections in English hospitals”. Journal of Hospital Infection2 (2005): 93-103.
  8. Sanjay KR., et al. “A study on isolation and detection of drug resistance gram negative bacilli with special importance to post-operative wound infection”. Journal of Microbiology and Antimicrobial6 (2010): 68-75.
  9. Cheesbrough M. “District laboratory practice in tropical countries, part 2”. Cambridge university press (2005).
  10. Munckhof W. “Antibiotics for surgical prophylaxis”. 28 (2005): 38-40.
  11. Nirmala S., et al. “Aerobic bacterial isolates and their antibiotic susceptibility pattern from pus samples in a tertiary care government hospital in Tamilnadu, India”. International Journal of Current Microbiology and Applied Science 6 (2017): 423-442.
  12. Merchand IA., et al. “Veterinary bacteriology and virology”. Veterinary Bacteriology and Virology (1967).
  13. Cheesbrough M. “Laboratory manual for tropical countries”. Volume II. Microbiology. Tropical Health Technology, ELBS, London, UK, (2003): 214-220.
  14. Gautman SK., et al. “Rapid and sensitive detection of Escherichia coli in milk by 16S rRNA gene targeted PCR”. International Journal of Animal Biotechnology1 (2011): 107-110.
  15. Sato T., et al. “Nested PCR for detection of mutans streptococci in dental plaque”. Letters in Applied Microbiology 1 (2003): 66-69.
  16. Kumar S., et al. “MEGA7: molecular evolutionary genetics analysis version 7.0 for bigger datasets”. Molecular Biology and Evolution7 (2016): 1870-1874.
  17. Saitou N., et al. “The neighbor-joining method: a new method for reconstructing phylogenetic trees”. Molecular Biology and Evolution4 (1987): 406-425.
  18. Baur AW., et al. “Antibiotic susceptibility testing by a standardized single disk method”. American Journal of Clinical Pathology4 (1966): 493-496.
  19. Clinical and Laboratory Standards Institute. “Performance standards for antimicrobial susceptibility testing”. CLSI Supplement M100 (2017).
  20. Kc R., et al. "Bacteriological study of wound infection and antibiotic susceptibility pattern of the isolates”. Nepal Journal of Science and Technology2 (2013): 143-150.
  21. Kaftandzieva A., et al. "Bacteriology of wound-clinical utility of gram stain microscopy and the correlation with culture”. Macedonian Journal of Medical Sciences (2012).
  22. Verma AK., et al. "Antimicrobial susceptibility pattern of bacterial isolates from surgical wound infections in tertiary care hospital in Allahabad, India”. Internet Journal of Medical Update-EJOURNAL1 (2012): 27-34.
  23. Zafar A., et al. “Bacteriology of infected wounds–A study conducted at children’s hospital Lahore”. Biomedica January-June (2008): 71-74.
  24. Godebo G., et al. "Multidrug-resistant bacterial isolates in infected wounds at Jimma University Specialized Hospital, Ethiopia”. Annals of Clinical Microbiology and Antimicrobials1 (2013): 1-7.
  25. Shriyan AR., et al. "Aerobic micro-organisms in post-operative wound infections and their antimicrobial susceptibility patterns”. Journal of Clinical Diagnosis Research6 (2010): 3392-396.
  26. Sani RA., et al. "Antibiotic resistance profile of gram positive bacteria isolated from wound infections in Minna, Bida, Kontagora and Suleja area of Niger State”. Journal of Health Science 2.3 (2012): 19-22.
  27. Rai S., et al. "Bacteriological profile and antimicrobial susceptibility patterns of bacteria isolated from pus/wound swab samples from children attending a tertiary care hospital in Kathmandu, Nepal”. International Journal of Microbiology2017 (2017): 1-5.
  28. Anguzu JR., et al. "Drug sensitivity patterns of bacterial isolates from septic post-operative wounds in a regional referral hospital in Uganda”. African Health Sciences3 (2007): 148-154.
  29. Guta M., et al. “Bacteria from infected surgical wounds and their antimicrobial resistance in Hawassa University Referral Teaching Hospital, Southern Ethiopia”. African Journal of Microbiology Research11 (2014): 1118-1124.
  30. Raza MS., et al. “Antimicrobial susceptibility patterns of the bacterial isolates in post-operative wound infections in a tertiary care hospital, Kathmandu, Nepal”. Open Journal of Medical Microbiology3 (2013): 159-163.

Citation

Citation: Nazmi Ara Rumi., et al. “Determination of Pathogenic Bacteria Isolated from Post-Operative Wound Infections and Their Antibiotic Resistance Pattern: A Hospital-Based Cross-Sectional Study". Acta Scientific Medical Sciences 7.2 (2023): 177-185.

Copyright

Copyright: © 2022 Nazmi Ara Rumi., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


Contact US