Kruti Rathod¹, Aarshvi Patel¹ and Rinkal Mulani²*

¹Department of Microbiology and Biotechnology, School of Sciences, Gujarat University, Ahmedabad, Gujarat, India
²Department of Biotechnology, Shri Alpesh N Patel Postgraduate Institute of Science and Research, Sardar Patel University, Anand, Gujarat, India

*Corresponding Author: Rinkal Mulani, Department of Biotechnology, Shri Alpesh N Patel Postgraduate Institute of Science and Research, Sardar Patel University, Anand, Gujarat, India.

Received: November 06, 2023; Published: November 20, 2023

Abstract

In practically all types of temperate and tropical soils, the fungus of the genus Trichoderma can be found. Additionally, they are found on decaying wood, bark, and other organic plant components. In this investigation, four distinct Trichoderma species were isolated from peanut rhizosphere soils (Arachis hypogaea). Isolates were taxonomically identified up to the species level using macro and micromorphological features. To identify the species of Trichoderma, morphological investigations such as pigmentation, colony growth, and anatomical studies such as conidiation appearances, size of conidia, conidiophores branching pattern, forms of phialides, and the absence or presence of chlamydospores were carried out. Based on macro, micro-morphological and 18s RNA sequencing analysis, Trichoderma isolates were classified into four species T. viride (GFT1), T. harzianum (GFT2), T. longibrachiatum (GFT3), and T. reesei (GFT4). Trichoderma harzianum species were prevalent in soil, with Trichoderma viren ranking second. A symbiont, the Rhizobium bacteria develops root nodules in association with the roots of leguminous plants. From the root nodules of Arachis hypogaea L., two bacterial strains were discovered. Three distinct confirmatory tests were carried out on the isolates as part of an investigation into their morphological and microscopic features. The biochemical features of the isolates were also examined. 16sRNA sequencing was used to provide taxonomy identification. Rhizobium leguminosarum PR7 and Rhizobium pusense PR4 were the two strains we obtained based on all the data. In the future, all isolated strains will be used for additional research on Trichoderma strains, including biocontrol and bioagumentor, and Rhizobium strains will be used to determine the effects of Trichoderma on Rhizobium under soil.

Keywords: Trichoderma spp; Rhizobium spp; Nodules; Confirmatory Test; Molecular Identification

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Citation

Citation: Rinkal Mulani., et al. “Isolation, Characterization and Molecular Identification of Rhizobium spp. and Trichoderma spp. from Nodule and Rhizospheric Soil of Arachis hypogaea".Acta Scientific Microbiology 6.12 (2023): 47-56.

Copyright

Copyright: © 2023 Rinkal Mulani., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.