Farhana Abdul-Samathu^1,2, Ting Yi Lim^1,2, Felice Jia Ying Ng^1,2, Nursakinah Mohamed-Khalid^1,2, Nicholas Wei Jun Liew^1,2 and Maurice HT Ling^2,3,4*

¹School of Health & Life Sciences, Teesside University, UK ²Management Development Institute of Singapore, Singapore ³Newcastle Australia Institute of Higher Education, University of Newcastle, Australia ⁴HOHY PTE LTD, Singapor

*Corresponding Author: Maurice HT Ling, Management Development Institute of Singapore, Singapore.

Received: September 07, 2026; Published: October 01, 2026

Abstract

Chromobacterium violaceum ATCC 12472, a Gram-negative bacterium, is primarily found in soil and freshwater, mainly in tropical or subtropical regions, thriving in warm climates. Despite being an opportunistic pathogen, C. violaceum ATCC 12472 is known to have therapeutic potential as well other useful qualities. It produces a violet pigment called violacein that carry anticancer as well anti-parasitic properties. It also produces hydrogen cyanide which can solubilize gold; thus, making it a potential target for metabolic engineering. In silico metabolic modelling utilizing genome-scale models (GSMs) and kinetic models (KMs) offers predictive insights to guide engineering strategies. Although GSMs are more widely implemented, KMs provide a superior, dynamic view of metabolic networks and allow for a more intuitive integration of simulated genetic modifications. Although a GSM of C. violaceum has been published, there had been no KM of C. violaceum to date. Therefore, this study aims to develop a whole-cell KM of C. violaceum ATCC 12472, The resulting KM, cviFA26, consists of 618 distinct enzyme commission (EC) numbers linked to 1736 enzymatic reactions involving 1255 metabolites. This serves as a foundational KM for future development.

Keywords: Whole-Cell Model; Kinetic Model; Opportunistic Pathogen; Violacein; Hydrogen Cyanide; Differential Equations; AdvanceSyn Toolkit

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Citation

Citation: Maurice HT Ling., et al. “Ab Initio Whole Cell Kinetic Model of Chromobacterium violaceum ATCC 12472 (cviFA26)". Acta Scientific Microbiology 9.10 (2026): 10-15.

Copyright

Copyright: © 2026 Maurice HT Ling., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.