R Vidhyalakshmi*, Praseeda Padmanabhan, Charmaine Antionette Shu and Fathima Munavar Ahamed Basheer
Department of Microbiology, Dr. MGR Janaki College of Arts and Science, Adyar, Chennai-28, Tamil Nadu, India
*Corresponding Author: R Vidhyalakshmi, Department of Microbiology, Dr. MGR Janaki College of Arts and Science, Adyar, Chennai-28, Tamil Nadu, India.
Received: May 12, 2025; Published: May 30, 2025
The Lion’s Mane mushroom (Hericium erinaceus) has garnered increasing scientific attention due to its potential health-promoting properties, notably its rich profile of bioactive compounds and its ability to stimulate probiotic growth. This study explores the chemical composition and biological activity of extracts derived from H. erinaceus using aqueous, ethanolic, and mixed solvent systems. Gas Chromatography-Mass Spectrometry (GC-MS) analysis identified 15 bioactive compounds across the extracts. Notably, the aqueous extract demonstrated the highest capacity to stimulate the growth of Lactobacillus sps, highlighting its potential as a stimulatory agent for promoting the growth of probiotic bacteria Lactobacillus sps. Linoleic acid (LA), a common omega-6 polyunsaturated fatty acid, has been identified in the lipid profile of Hericium erinaceus (Lion’s Mane mushroom) extracts, contributing to the mushroom’s pharmacological properties. Although LA is not exclusive to Lion’s Mane, its presence is well-documented and is known to play a role in modulating cellular functions such as membrane fluidity, oxidative stress, and inflammatory responses. Recent molecular docking studies revealed that LA exhibits a favourable binding affinity toward cathepsin D, a lysosomal aspartic protease implicated in tumour progression, metastasis, and poor prognosis in several cancers. The observed binding interactions suggest potential inhibitory effects, particularly if LA engages key residues within the catalytic domain of cathepsin D. This characteristic could offer relief to chapped nipples and also a wide range of inflammatory conditions, hinting at its potential for future pharmaceutical applications. Docking studies on linoleic acid and cathepsin D protein revealed promising therapeutic interactions, supported by a favourable dock score of -6.06 and the observation of a hydrogen bond between the ligand and protein. Additionally, the mushroom extracts displayed significant anti-inflammatory properties, as demonstrated by an albumin denaturation assay, with the highest percentage inhibition recorded at 63.09%This research underscores the diverse potential of Lion's Mane mushroom, spanning from probiotic growth stimulation to anti-inflammatory, thereby contributing valuable insights to the understanding of this remarkable fungus used in traditional foods in many countries.
Keywords: Lion's Mane Mushroom; Probiotic Stimulants; Cathepsin D; Bioactive Compounds
Citation: R Vidhyalakshmi. “Role of Hericium erinaceus as a Probiotic Stimulant".Acta Scientific Microbiology 8.6 (2025): 53-61.
Copyright: © 2025 R Vidhyalakshmi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.