Nicole Prisca Makaya Dangui Nieko^1,2*, Christian Aimé Kayath³, Moїse Doria Kaya-Ongoto^1,3, Duchel Jeanedvi Kinouani Kinavouidi^1,3 and Fabien Roch Niama^1,3

¹Service de Bactériologie, Laboratoire National de Santé Publique, BP 120, Brazzaville, Congo
²Ecole Normale Supérieure, Université Marien Ngouabi, BP 69, Brazzaville, Congo
³Faculté des Sciences et Techniques, Université Marien Ngouabi, BP 69, Brazzaville, Congo

*Corresponding Author: Nicole Prisca Makaya Dangui Nieko, Service de Bactériologie, Laboratoire National de Santé Publique, BP 120, Brazzaville, Congo.

Received: November 18, 2024; Published: December 11, 2024

Abstract

Escherichia coli is the most frequently isolated pathogen of urogenital infections. Its virulence gives it the ability to evade host defences and develop resistance to antibiotics. The aim of this work is to detect virulence factors and correlate them with antibiotic resistance. The virulence genes were detected by multiplex PCR. Susceptibility to antibiotics was tested by diffusion of agar discs. Of the 102 isolated strains, 43.14% had at least one of the three virulence factors tested. The sfa/foc gene was the most predominant, with a rate of 59.10%. The antibiotic susceptibility test showed the overall resistance of the strains tested, ranging from 20 to 80%. The correlation between antibiotic resistance and the expression of virulence factors showed that strains carrying the afa and sfa/ foc genes were resistant to amoxicillin/clavulanic acid at rates of 83.3% and 61.5%, respectively; strains carrying the pap gene were 95% resistant to imipenem. Statistically significant P values were obtained, respectively, 0.001 for sfa/foc, 0.034 for afa, and 0.000 for pap. Other statistically significant results were also obtained. These factor detection rates and their antibiotic resistance profiles should lead us to question the hygiene measures to be taken to avoid contamination of the urogenital tract by these factors.

Keywords:E. coli; Antimicrobial Resistance; Virulence Factors

References

1. Whelan S., et al. “Uropathogenic Escherichia coli (UPEC)-Associated Urinary Tract Infections: The Molecular Basis for Challenges to Effective Treatment”. Microorganisms9 (2023): 2169.
2. Darmancier H., et al. “Are Virulence and Antibiotic Resistance Genes Linked? A Comprehensive Analysis of Bacterial Chromosomes and Plasmids”. Antibiotics (Basel)6 (2022): 706.
3. Garnotel E., et al. “Sensibilité aux antibiotiques d’Escherichia coli isolé des infections urinaires Communautaires: étude AFORCOPI-BIO, 2015”. Revue Francophone des laboratoires 496 (2017): 66-73.
4. Tan CW and Chlebicki MP. “Urinary tract infections in adults”. Singapore Medical Journal9 (2016): 485-490.
5. Maris Ségolène 2016. Caractérisation de souches d’Escherichia coli pathogènes Urinaires Provenant De Guadeloupe : Portrait de la diversité des facteurs de virulence Présents”. Mémoire de Maîtrise en Microbiologie Appliquée de l’Université du Québec, Canada (2016): 113.
6. Pakbin B., et al. “Virulence Factors of Enteric Pathogenic Escherichia coli: A Review”. International Journal of Molecular Sciences 18 (2021): 9922.
7. Fatima S., et al. “Virulence Factors and Antimicrobial Resistance of Uropathogenic Escherichia coli EQ101 UPEC Isolated from UTI Patient in Quetta, Balochistan, Pakistan”. Biomed Research International (2023): 7278070.
8. Basavaraju M and Gunashree BS. “Escherichia coli: An Overview of Main Characteristics”. IntechOpen (2023).
9. Rahdar M., et al. “Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From Patients With Urinary Tract Infection”. Jundishapur Journal of Microbiology8 (2015): e22647.
10. de Oliveira H C., et al. “Importance of adhesins in virulence of Paracoccidioides spp”. Frontiers in Microbiology 6 (2015).
11. Klein RD and Hultgren SJ. “Urinary tract infections: microbial pathogenesis, host-pathogen interactions and new treatment strategies”. Nature Review Microbiology4 (2020): 211-226.
12. Nisha K., et al. “Comparative analysis of virulence factors & biotypes of Gardnerella vaginalis isolated from the genital tract of women with & without bacterial vaginosis”. Indian Journal of Medical Research1 (2019): 57-61.
13. Srilakshmi Yarlagadda Sajana G., et al. “Association of vaginal infections in Preterm labour”. International Journal of Reproduction, Contraception, Obstetrics and Gynecology 6 (2018): 2174-2179.
14. Salam MA., et al. “Antimicrobial Resistance: A Growing Serious Threat for Global Public Health”. Healthcare 13 (2023): 1946.
15. Abed AD and Mutter TY. “Relationship between antimicrobial resistance and virulence factors in uropathogenic Escherichia coli isolates from Ramadi, Iraq: phenotype and genotype identification”. African Health Science3 (2023): 486-496.
16. Makaya Dangui Nieko NP., et al. “Phenotypic and Genotypic Characterization of Metallo-Beta-Lactamase and Extended-Spectrum Beta-Lactamase among Enterobacteria Isolated at National Public Health Laboratory of Brazzaville”. Advances in Microbiology3 (2022): 63-377.
17. Guenadez Maya Ouided. “Profil épidémiologique des entérobactéries uropathogènes sécrétrices de BLSE”. Mémoire de Master de l’Université des Frères Mentouri Constantine (2020).
18. Michel Claire 2014. “Infections urinaires gravidiques : analyse de leur prise en charge dans le service d’hospitalisation des grossesses du CHU Estaing à Clermont-Ferrand”. Thèse de Doctorat de l’université de l’Université d’Auvergne-Clermont 1 (2014): 75.
19. Thomas-White K., et al. “Culturing of female bladder bacteria reveals an interconnected urogenital microbiota”. Nature Communication1 (2018): 1557.
20. Lewis AL and Gilbert NM. “Roles of the vagina and the vaginal microbiota in urinary tract infection: evidence from clinical correlations and experimental models”. GMS Infectious Disease 8 (2020): Doc02.
21. Mueller Mathieu and Tainter Christopher R. “Escherichia coli Infection”. In: StatPearls. Treasure Island (FL): StatPearls Publishing (2024).
22. Safarpoor Dehkordi F., et al. “Uropathogenic Escherichia coli in the high vaginal swab samples of fertile and infertile women: virulence factors, O-serogroups, and phenotyping and genotyping characterization of antibiotic resistance”. New Microbes and New Infectious 38 (2020): 100824.
23. Aleksandrowicz A., et al. “Whatever makes them stick-Adhesins of avian pathogenic Escherichia coli”. Veterinary Microbiology 257 (2021): 109095.
24. Helmy YA., et al. “Antimicrobial Resistance and Recent Alternatives to Antibiotics for the Control of Bacterial Pathogens with an Emphasis on Foodborne Pathogens”. Antibiotics (Basel)2 (2023): 274.
25. El-Far SW and Abukhatwah MW. “Prevalence of Aminoglycoside Resistance Genes in Clinical Isolates of Pseudomonas aeruginosa from Taif, Saudi Arabia-An Emergence Indicative Study”. Microorganisms9 (2023): 2293.
26. Ramirez MS and Tolmasky ME. “Aminoglycoside modifying enzymes”. Drug Resistance Update6 (2010): 151-171.
27. Zhang Y., et al. “The prevalence and distribution of aminoglycoside resistance genes”. Biosafety and Health 1 (2023): 14-20.
28. López M., et al. “Resistance to Fluoroquinolones in Pseudomonas aeruginosa from Human, Animal, Food and Environmental Origin: The Role of CrpP and Mobilizable ICEs”. Antibiotics (Basel)9 (2022): 1271.
29. Hooper DC and Jacoby GA. “Topoisomerase Inhibitors: Fluoroquinolone Mechanisms of Action and Resistance”. Cold Spring Harbor Perspectives in Medicine 6 (2016).
30. Miranda-Estrada LI., et al. “Relationship between virulence factors, resistance to antibiotics and phylogenetic groups of uropathogenic Escherichia coli in two locations in Mexico”. Enfermedades Infecciosas y Microbiología Clínica 7 (2017): 426-433.

Citation

Citation: Nicole Prisca Makaya Dangui Nieko., et al. “First detection of virulence factors in Escherichia coli isolated from urogenital tract and correlation with antimicrobial resistance at the National Public Health Laboratory (Brazzaville)". Acta Scientific Microbiology 8.1 (2025): 33-40.

Copyright

Copyright: © 2025 Nicole Prisca Makaya Dangui Nieko., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.