Anila Joseph, Nancy Ann Joseph S, Preetha Nair*

Department of Biotechnology, Mount Carmel College Autonomous, Bengaluru, India

*Corresponding Author: Preetha Nair, Department of Biotechnology, Mount Carmel College Autonomous, Bengaluru, India.

Received: November 06, 2024; Published: December 05, 2024

Abstract

For the past few decades, bioactive compounds from marine organisms have been utilized for their therapeutic activity. Among algae, microalgae are extensively studied and reported to exhibit more diversity in their pharmacological action. Our attempt is to explore the profile of the biologically important active molecules in macroalgae from the marine environment. Simultaneously we also investigated the therapeutic potential of algal extracts. The results may open new avenues for medical research and offer substitute treatments for illnesses linked to oxidative stress and inflammatory diseases. The methodologies consist of two phases, the first stage consists of algal culturing and staining, extraction of phytonutrients and their estimations. We have compared three marine algal species, Padina pavonica, Ulva fasciata and Hypnea valentiae. The phytochemical profile including alkaloids, tannin, phenol, saponin, and flavonoids along with major biomolecules (protein, carbohydrate, and lipid) was analyzed in all three algal extracts. The second phase consists of the assessment of the therapeutic potential of marine algal extract. Anti-inflammatory, anti arthritic, and anti-oxidant potential along with the antiproliferative effect on UV-exposed mammalian cells was assessed. Its rich nutritional profile has been highlighted by examining its biochemical composition, which positions algae as a sustainable source of protein and other essential nutrients for human and animal feed. Study indicates that the compounds derived from algae can regulate inflammatory pathways, prevent oxidative stress, and mitigate arthritic symptoms. This makes marine algae a viable and sustainable substitute for traditional pharmaceutical treatments. Furthermore, the safety profile of algal elements offers minimal side effects compared to synthetic medications. Further investigation into the phytochemical composition and therapeutic mechanisms of algae can reveal their potential in healthcare and quality of life.

Keywords:Protein; Carbohydrate; Algae

References

1. Fakayode AE. “Phytonutrients, antioxidants, and anti-inflammatory analysis of Peperomia pellucida”. Journal of Medical Pharmaceutical and Allied Sciences 10 (2021): 3517-3523.
2. Shakoor H., et al. “Immunomodulatory Effects of Dietary polyphenols”. Nutrients 13 (2021): 728.
3. Rezayian M., et al. “Oxidative damage and antioxidative system in algae”. Toxicology Reports 6 (2019): 1309-1313.
4. Provasoli L., et al. “The development of artificial media for marine algae”. Archives of Microbiology 25 (1957): 392-428.
5. Nguyen TV. “Preparation of Artificial Sea Water (ASW) for Culturing Marine Bacteria”. (2018): 10.
6. Ma Y., et al. “Staining of paraffin-embedded plant material in safranin and fast green without prior removal of the paraffin”. Canadian Journal of Botany 71 (2011): 996-999.
7. Slocombe SP., et al. “A rapid and general method for measurement of protein in micro-algal biomass”. Bioresource Technology 129 (2013): 51-57.
8. Axelsson M and F Gentili. “A Single-Step Method for Rapid Extraction of Total Lipids from Green Microalgae”. PLoS ONE 9 (2014): e89643.
9. Abd El-Baky H and G El-Baroty. “Algal extracts improve antioxidant defense abilities and salt tolerance of wheat plant irrigated with seawater”. African Journal of Biochemistry Research 2 (2008): 151-164.
10. Layne E. “Spectrophotometric and Turbidimetric Methods for Measuring Proteins”. Methods in Enzymology 10 (1957): 447-455.
11. Kumar R R., et al. “Lipid Extraction Methods from Microalgae: A Comprehensive Review”. Frontiers in Energy Research 2 (2015): 1-9.
12. Yue F., et al. “Effects of monosaccharide composition on quantitative analysis of total sugar content by phenol-sulfuric acid method”. Frontiers in Nutrition 9 (2022): 1-10.
13. Senguttuvan J., et al. “Phytochemical analysis and evaluation of leaf and root parts of the medicinal herb, Hypochaeris radicata L. for in vitro antioxidant activities”. Asian Pacific Journal of Tropical Biomedicine 4 (2014): S359-S367.
14. Siddhuraju P and K Becker. “Antioxidant Properties of Various Solvent Extracts of Total Phenolic Constituents from Three Different Agroclimatic Origins of Drumstick Tree (Moringa oleifera Lam.) Leaves”. Journal of Agricultural and Food Chemistry 51 (2003): 2144-2155.
15. Zhishen J., et al. “The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals”. Food Chemistry 64 (1999): 555-559.
16. Nithiyanantham S., et al. “Differential Effects of Processing Methods on Total Phenolic Content, Antioxidant and Antimicrobial Activities of Three Species of Solanum”. Journal of Food and Drug Analysis 20 (2012) :21.
17. Le A., et al. “Improving the Vanillin-Sulphuric Acid Method for Quantifying Total Saponins”. Technologies 6 (2018) : 84.
18. Baliyan S., et al. “Determination of Antioxidants by DPPH Radical Scavenging Activity and Quantitative Phytochemical Analysis of Ficus religiosa”. Molecules 27 (2022): 1326.
19. Yesmin, et al. “Membrane stabilization as a mechanism of the anti-inflammatory activity of ethanolic root extract of Choi (Piper chaba)”. Clinical Phytoscience 6 (2020): 59.
20. Chandra S., et al. “Evaluation of invitro anti-inflammatory activity of coffee against the denaturation of protein”. Asian Pacific Journal of Tropical Biomedicine15 (2012): S178-S180.
21. Louis KS., et al. “Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods”. In: Stoddart, M. (eds) Mammalian Cell Viability. Methods in Molecular Biology vol 740 (2011).
22. Mucha P., et al. “Overview of the Antioxidant and Anti-Inflammatory Activities of Selected Plant Compounds and Their Metal Ions Complexes”. Molecules 26 (2021): 4886.
23. Zeb A. “Concept, mechanism, and applications of phenolic antioxidants in foods”. Journal of Food Biochemistry 44 (2020): e13394. 
24. Rahman , et al. “Role of Phenolic Compounds in Human Disease: Current Knowledge and Future Prospects”. Molecules 27 (2021): 233. 
25. Huang J., et al. “Tannins as antimicrobial agents: Understanding toxic effects on pathogens”. Toxicon 24 (2024): 107812.
26. Safe, et al. “Flavonoids: structure-function and mechanisms of action and opportunities for drug development”. Toxicology Research37 (2021): 147-162. 
27. Desai S., et al. “Saponins and their biological activities”. Pharma Times 41 (2009): 13-16.
28. Pisoschi AM., et al. “Comprehensive and critical view on the anti-inflammatory and immunomodulatory role of natural phenolic antioxidants”. European Journal of Medicinal Chemistry 265 (2024): 116075.
29. Chen L., et al. “Inflammatory responses and inflammation-associated diseases in organs”. Oncotarget 9 (2017): 7204-7218.
30. Pradhan, et al. “Bioactive Metabolites from Marine Algae as Potent Pharmacophores against Oxidative Stress-Associated Human Diseases: A Comprehensive Review”. Molecules 26 (2020): 37. 
31. Hessling, et al. “The impact of far-UVC radiation (200-230 nm) on pathogens, cells, skin, and eyes - a collection and analysis of a hundred years of data”. GMS Hygiene and Infection Control 16 (2021). 
32. Pfeifer GP., et al. “Mutations induced by ultraviolet light”. Mutation Research 571 (2005) : 19-31.
33. de Jager TL., et al. “Ultraviolet Light Induced Generation of Reactive Oxygen Species”. Advances in Experimental Medicine and Biology 996 (2017): 15-23. 
34. Kan J., et al. “Phytonutrients: Sources, bioavailability, interaction with gut microbiota, and their impacts on human health”. Frontiers in Nutrition 9 (2022): 1-21.

Citation

Citation: Preetha Nair., et al. “Efficacy of Bioactive Compounds Extracted from Marine Algae". Acta Scientific Microbiology 8.1 (2025): 11-23.

Copyright

Copyright: © 2025 Preetha Nair., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.