Acta Scientific Microbiology

Research Article Volume 7 Issue 8

The Analysis of Cytotoxicity of Durban Residential House Dust Extracts on Human Lung Bronchial Epithelial Cell Line (A549) and Human Lung Bronchus Virus Transformed Epithelial Cell Line (ATCC No. CRL – 9482 BBM)

Bongani Nkala1,2* and Nceba Gqaleni2

1Faculty of Applied Science, Eduvos, Glen Austin AH, Midrand, South Africa
2Department of Traditional Medicine, University of KwaZulu-Natal, South Africa

*Corresponding Author: Bongani Nkala, Faculty of Applied Science, Eduvos, Glen Austin AH, Midrand, South Africa.

Received: May 10, 2024; Published: July 29, 2024

Abstract

There has been increased focus on house dust due to its potential to contain biological pollutants in indoor environments. Biological factors may include pollen, spores, moulds, bacteria, viruses, allergens, dust mites, and epithelial cells. This study aimed to analyse the cytotoxicity of house dust on the human lung bronchus carcinoma epithelial cell line (A549) and human lung bronchus virustransformed epithelial cell line (BBM). One hundred and five (n = 105) house dust samples were obtained from households. Human cell lines were treated with five different dilutions of each house dust extract. Cell viability was assessed using the MTT assay. Toxic effects of house dust extract were analysed, following house dust extract treatment and cells were stained with double dye (annexin – V- and propidium iodide) and analysed with flow cytometry and fluorescent microscope. The results suggest that this study showed that dust extract is toxic to human cell lines and cells underwent a degree of apoptosis and necrosis at 62% (A549) and 99% (BBM). The findings in this study strongly suggest that house dust extracts are toxic to human cell lines.

Keywords: House Dust; A549 Cell Line; BBM Cell Line; MTT; Apoptosis; Necrosis

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Citation

Citation: Bongani Nkala and Nceba Gqaleni. “The Analysis of Cytotoxicity of Durban Residential House Dust Extracts on Human Lung Bronchial Epithelial Cell Line (A549) and Human Lung Bronchus Virus Transformed Epithelial Cell Line (ATCC No. CRL – 9482 BBM)".Acta Scientific Microbiology 7.8 (2024): 188-194.

Copyright

Copyright: © 2024 Bongani Nkala and Nceba Gqaleni. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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