Taliby Dos Camara^1,2*, Abdoulaye Makanera³, Fassou René Kolié^1,2, César Hagoussou¹ and Fodé Assé Camara⁴

¹Microbiology Laboratory, Department of Biology, Faculty of Science, Gamal Abdel Nasser University, Conakry, Republic of Guinea
²Medical Biology Laboratory, Mahatma Gandhi University, Conakry, Republic of Guinea
³Laboratory of the Faculty of Health Sciences and Techniques, Gamal Abdel Nasser University, Conakry, Republic of Guinea
⁴Siguri Prefectural Hospital Medical Biology Laboratory, Republic of Guinea

*Corresponding Author: Taliby Dos Camara, Lecturer and Researcher, Microbiology Laboratory, Department of Biology, Faculty of Science, Gamal Abdel Nasser University, Conakry, Republic of Guinea.

Received: April 15, 2024; Published: June 24, 2024

Abstract

Introduction: Tuberculosis (TB) is an infectious disease transmitted from person to person and caused by the bacillus Koch (BK). It is a major public health problem throughout the world, despite the numerous tuberculosis control strategies (TCS).

Objective: To evaluate the value of Genexpert MTB/RIF in the diagnosis of pulmonary tuberculosis in PLHIV at Siguiri prefectural hospital, with a view to improving patient care.

Method: This is a prospective, descriptive, cross-sectional study lasting six months, from 15 February to 15 August 2023.

Results: Biological diagnosis of 200 patients revealed 85 Genexpert-positive patients, i.e. a prevalence of 42.5%, compared with 79 fluorescence microscopy-positive patients, i.e. 39.5%. The variation in viral load in the 85 patients tested positive for HIV-TB co-infection was significant in HIV-TB co-infected patients with a mean of (59277copies/ml). Immunosuppression was advanced overall, with the majority of patients in our study having an LTCD4+ level of less than 200 cells/μl. The mean LTCD4+ level was 124.52 cells/μl, with extremes ranging from 5 to 349 cells/μl. Patients with a mean LTCD4+ level ≥ 200 cells/μl accounted for 72% of cases. Married patients were the most represented at 62%, the majority being blue-collar workers (42%), followed by housewives (19%). The 31-40 age group was the most affected, with a prevalence of 31%. The Siguiri Koura 1 and 2 neighbourhoods were the most affected by HIV-MTB co-infection, with 26% and 21% respectively.

Conclusion: The present study proves that the Genexpert MTB/RIF method is the best method for diagnosing pulmonary tuberculosis compared with Microscopy (Fluorescence and Ordinary) and plays a major role in predicting multi-drug resistance, particularly to Rifampicin, in Mycobacterium tuberculosis. Its systematic use in conjunction with.

Keywords: HIV; Tuberculosis; Microscopy; PCR GeneXpert MTB/RIF; Siguiri

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Citation

Citation: Taliby Dos Camara., et al. “Genexpert MTB/RIF Contribution to the Diagnosis of Pulmonary Tuberculosis in People Living with HIV at the Siguiri Prefectural Hospital (Republic of Guinea)".Acta Scientific Microbiology 7.7 (2024): 76-85.

Copyright

Copyright: © 2024 Taliby Dos Camara., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.