Lamlom Taie Lamlom Tahawy, Ari Susilowati and Sutarno Sutarno

Bioscience Study Program, Graduate Program, Universitas Sebelas Maret, Surakarta, Indonesia

*Corresponding Author: Lamlom Taie Lamlom Tahawy, Bioscience Study Program, Graduate Program, Universitas Sebelas Maret, Surakarta, Indonesia.

Received: May 30, 2024; Published: June 20, 2024

Abstract

This study presents a comprehensive exploration into the genetic diversity and chemical composition of Centella asiatica, a medicinal plant of significant pharmacological interest, harvested from three distinct locales within the rich biodiversity hotspot of Central Java, Indonesia. Through meticulous analysis, we scrutinized a total of sixteen samples, employing advanced techniques such as genomic DNA extraction and electrophoretic profiling. Our findings unveil a diverse array of banding patterns across the sampled populations, indicative of substantial genetic variations within Centella asiatica. Concurrently, quantitative assessments of saponin and terpenoid content provide invaluable insights into the biochemical richness of these plant specimens. Moreover, environmental parameters including temperature, soil pH, humidity, and lux meter readings were meticulously recorded, facilitating a comprehensive understanding of their influence on the plant's phytochemistry. This interdisciplinary approach allows for a nuanced exploration of Centella asiatica genetic makeup, chemical constituents, and environmental interactions, providing a solid foundation for further research into its therapeutic potential and ecological significance.

Keywords: Centella asiatica; Genetic Diversity; Electrophoresis; Saponins; Terpenoids; Environmental Factors

References

1. Baek S., et al. “Development of Hairy Root Cultures for Biomass and Triterpenoid Production in Centella asiatica”. Plants2 (2022).
2. Ganie IB., et al. “Biotechnological Intervention and Secondary Metabolite Production in Centella asiatica L”. In Plants 11.21 (2022a).
3. Butterfield D A., et al. “Pharmacokinetics and Pharmacodynamics of Key Components of a Standardized Centella asiatica Product in Cognitively Impaired Older Adults: A Phase 1, Double-Blind, Randomized Clinical Trial”. (2022a).
4. Sanguansajapong V., et al. “Development of Oral Microemulsion Spray Containing Pentacyclic Triterpenes-Rich Centella asiatica (L.) Urb. Extract for Healing Mouth Ulcers”. Pharmaceutics 11 (2022).
5. Li M., et al. “Probing Pharmaceutical Strategies to Promote the Skin Delivery of Asiatic Acid from Hydrogels: Enhancement Effects of Organic Amine Counterions, Chemical Enhancers, and Microneedle Pretreatment”. Pharmaceutics11 (2022).
6. Wright KM., et al. “Developing a Rational, Optimized Product of Centella asiatica for Examination in Clinical Trials: Real World Challenges”. Frontiers in Nutrition 8 (2022a).
7. Hebbar S., et al. “Cationic biopolymer decorated Asiatic Acid and Centella asiatica extract incorporated liposomes for treating early-stage Alzheimer’s disease: An In-vitro and In-vivo investigation”. F1000Research 11 (2022).
8. Arribas-López E., et al. “A Systematic Review of the Effect of Centella asiatica on Wound Healing”. In International Journal of Environmental Research and Public Health 19.6 (2022).
9. Cabey K., et al. “Withania somnifera and Centella asiatica Extracts Ameliorate Behavioral Deficits in an In Vivo Drosophila melanogaster Model of Oxidative Stress”. Antioxidants1 (2022).
10. Wright K M., et al. “Developing a Rational, Optimized Product of Centella asiatica for Examination in Clinical Trials: Real World Challenges”. Frontiers in Nutrition 8 (2022a).
11. Kwak SY., et al. “Centella asiatica-Derived Endothelial Paracrine Restores Epithelial Barrier Dysfunction in Radiation-Induced Enteritis”. Cells16 (2022).
12. Djakaria S A., et al. “Antioxidant and Antibacterial Activity of Selected Indonesian Honey against Bacteria of Acne”. Jurnal Kimia Sains Dan Aplikasi8 (2020): 267–275.
13. Khwanchum L., et al. “Differentiation of Centella asiatica (L.) Urb. from Hydrocotyle umbellata L. using the trnH-psbA region, species-specific bands and anisocytic stomata as markers for quality control of raw materials and their products”. Journal of Applied Research on Medicinal and Aromatic Plants 37 (2023b).
14. Khwanchum L., et al. “Differentiation of Centella asiatica (L.) Urb. from Hydrocotyle umbellata L. using the trnH-psbA region, species-specific bands and anisocytic stomata as markers for quality control of raw materials and their products”. Journal of Applied Research on Medicinal and Aromatic Plants (2023a): 37.
15. Sellathoroe S. “COMPARISON OF DIFFERENT EXTRACTION METHODS TO STUDY THE ANTIMICROBIAL ACTIVITY OF CENTELLA ASIATICA LEAF EXTRACTS”. International Journal of Advanced Research5 (2019): 344-347.
16. Biradar SR. “Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella asiatica L. (URB)”. American Journal of Life Sciences6 (2013a): 243.
17. Biradar SR. “Extraction of Some Secondary Metabolites &Thin Layer Chromatography from Different Parts of Centella asiatica L. (URB)”. American Journal of Life Sciences6 (2013b): 243.

Citation

Citation: Lamlom Taie Lamlom Tahawy., et al. “Genetic Diversity and Phytochemical Analysis of Centella asiatica in Central Java: A Study Based on Electrophoretic Profiling and Environmental Factors". Acta Scientific Microbiology 7.7 (2024): 51-57.

Copyright

Copyright: © 2024 Lamlom Taie Lamlom Tahawy., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.