Acta Scientific Microbiology

Review Article Volume 7 Issue 5

Gene Editing Technique: Homologue Recombination, Antisense mRNA, RNAi, Site-Directed Mutagenesis, and CRISPR/Cas9

Abdulghani A Naeem1,2, Saud A Abdulsamad1,2*, Youqiang Ke3 and Gang He4

1Basic Sciences Department, College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia
2King Abdullah International Medical Research Center, Jeddah, Saudi Arabia
3Department of Molecular and Clinical Cancer Medicine, Liverpool University, Liverpool, UK
4Sichuan Industrial Institute of Antibiotics, Chengdu University, Chengdu, Sichuan, China

*Corresponding Author: Saud A Abdulsamad, Basic Sciences Department, College of Science and Health Professions, King Saud bin Abdulaziz University for Health Sciences, Jeddah, Saudi Arabia.

Received: March 07, 2024; Published: April 26, 2024


Since the 1970s, molecular scientists have been searching for methods to modify DNA via biological repair processes. Thus, they would be able to fix genetic mutations or introduce new functions into the genome. In order to accomplish this goal, genome editing techniques were created. It has become considerably easier, more accurate, and less costly to modify the genome because to the advent of new techniques. There has been a surge in interest in genome editing's potential uses since these breakthroughs, both for basic research and for improving human health through treating or preventing illness and disability. Scientists may now modify DNA to alter physical characteristics like eye color and the likelihood of contracting illness thanks to advances in genome editing technology. Various methods are used by scientists to do this. Every medical advancement has its own set of advantages, hazards, regulatory difficulties and ethical issues that must be taken into consideration while using it. Genome editing raises a number of important challenges, including how to balance possible advantages against the danger of unintended damage; how to manage the usage of these techniques; how to include cultural values into pertinent medical and economic considerations; as well as how to respect the unavoidable disparities, founded in country cultures, that will impact attitudes on whether as well as how to utilize these technologies. The purpose of this study is to discuss the most recent advances in gene editing, as well as the potential benefits and drawbacks that may result from its use.

Keywords: Gene Editing; Homologue; Antisense; mRNA; RNAi; Mutagenesis


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Citation: Saud A Abdulsamad., et al. “Gene Editing Technique: Homologue Recombination, Antisense mRNA, RNAi, Site-Directed Mutagenesis, and CRISPR/Cas9".Acta Scientific Microbiology 7.5 (2024): 35-45.


Copyright: © 2024 Saud A Abdulsamad., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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