Acta Scientific Microbiology

Review Article Volume 7 Issue 3

A Composite Review on: Microbial Culture and Growth Curve of Bacteria

Kamalraj Mohan1, Gautam Srinivas KP2, Suresh Arumugam3, Pradeep Raj Meenakshi Sundaram8, Anandan Balakrishnan9, Varshini GopalaKrishnan4, Ignatius Roshan R4, Gokul G Ra5, Sathishkumar Elumalai6, Jeevan Malaiyan4,6,7*

11Department of Microbiology, Meenakshi Medical College Hospital and Research Institute, Meenakshi Academy of Higher Education and Research, Kancheepuram, India
2Department of General Medicine, Meenakshi Medical College Hospital and Research Institute, Meenakshi Academy of Higher Education and Research, Kancheepuram, India
3Central Research Facility, Meenakshi Medical College Hospital and Research Institute, Meenakshi Academy of Higher Education and Research, Kancheepuram, India
4Division of Virology, Department of Microbiology, Sri Muthukumaran Medical College Hospital and Research Institute, Affiliated to The Tamil Nadu Dr. M.G.R. Medical University, Chikkarayapuram, Chennai, India
5Department of General Medicine, Sri Muthukumaran Medical College Hospital and Research Institute, Affiliated to The Tamil Nadu Dr. M.G.R. Medical University, Chikkarayapuram, Chennai, India
6Central Research Facility, Sri Muthukumaran Medical College Hospital and Research Institute, Affiliated to The Tamil Nadu Dr. M.G.R. Medical University, Chikkarayapuram, Chennai, India
7Department of Microbiology, Annaii Medical College and Hospital, College Road, Pennalur, Sriperumbudur, Chennai, Tamil Nadu, India
8Department of General Medicine, Annaii Medical College and Hospital, College Road, Pennalur, Sriperumbudur, Chennai, Tamil Nadu, India
9Department of Genetics, Dr. ALM Post Graduate Institute of Basic Medical Sciences, University of Madras, Taramani campus, Chennai, India

*Corresponding Author: Jeevan Malaiyan, DDivision of Virology, Department of Microbiology and Central Research Facility, Sri Muthukumaran Medical College Hospital and Research Institute, Affiliated to The Tamil Nadu Dr. M.G.R. Medical University, Chikkarayapuram, Chennai, India.

Received: January 22, 2024; Published: February 11, 2024

Abstract

Objectives: The classic presentation of dengue fever has expanded its horizon by involving severe forms as atypical manifestations and hemorrhagic fever with reason unknown. This study aimed to characterize dengue viruses (DENV) causing hemorrhagic fever.

Methods: Clinically suspected cases of dengue fever were confirmed by NS1 antigen, IgM and IgG antibody. Following clinical confirmation dengue virus isolates were subjected to reverse transcriptase polymerase chain reaction (RT-PCR), sequenced, aligned using Clustal W and serotype based phylogenetic analysis was performed.

Results: A total of 308 cases were screened, during 2015-18 of which 106 (34%) samples were positive for dengue infection. 42 (40%) cases showed atypical dengue manifestations, 14 (13%) presented with hemorrhagic fever. Among the atypical manifestations, hepatomegaly and splenomegaly were the most common, followed by ascites 12 (11%), pleural effusion 9 (8%), bradycardia 6 (6%), meningitis 6 (6%), acalculous cholecystitis 04 (4%), haemoptysis 3 (3%) and acute pancreatitis 2 (2%). Dengue hemorrhagic fever (n = 14) was caused by, DENV-1 (n = 7), DENV-2 (n = 5) and DENV- 4 (n = 2). Serotype DENV-3 was not isolated in any of the samples. A new clade of genotype I (GI) was predominant in DENV-1; Genotype IVC (GIVC)was predominant in DENV-2 showing mixed clustering suggesting lineage C; clade B of lineage C, genotype I (GI)was seen in DENV-4. The amino acid changes were observed in M51I, L72F, S93N in DENV-1 and M104V, V112A in DENV-2 of C-prM regions of hemorrhagic isolates when compared to standard strains.

Conclusions: Multiple amino acid changes may have influenced the emergence of new clade, lineages and resulted in higher magnitude of severity dengue and presented as hemorrhagic fever (DHF).

Keywords: Atypical Manifestation; Characterization; Dengue Viruses; Hemorrhagic Fever; Phylogenetic Analysis

References

    1. World Health Organization. “Global strategy for dengue prevention and control 2012-2020”.
    2. Wang WH., et al. “Dengue hemorrhagic fever a systemic literature review of current perspectives on pathogenesis, prevention and control”. Journal of Microbiology, Immunology and Infection 6 (2020): 963-978.
    3. Ahamed SF., et al. “Emergence of new genotypes and lineages of dengue viruses during the 2012–15 epidemics in southern India”. International Journal of Infectious Diseases 84S (2019): S34-43.
    4. Gallagher P., et al. “The association of obesity and severe dengue: possible pathophysiological mechanisms”. Journal of Infectious1 (2020): 10-16.
    5. Harapan H., et al. “Dengue: A minireview”. Viruses 8 (2020): 829.
    6. Holmes EC and Twiddy SS. “The origin, emergence and evolutionary genetics of dengue virus”. Infection, Genetics and Evolution 1 (2003): 19-28.
    7. Bhatt P., et al. “Current Understanding of the Pathogenesis of Dengue Virus Infection”. Current Microbiology1 (2021): 17-32.
    8. Shrivastava A., et al. “Lineage shift of dengue virus in Eastern India: an increased implication for DHF/DSS”. Epidemiology Infectious8 (2015): 1599-1605.
    9. Kadam DB., et al. “Expanded Dengue”. Journal of the Association of Physicians of India7 (2016): 59-63.
    10. Fatima Z., et al. “Serotype and genotype analysis of dengue virus by sequencing followed by phylogenetic analysis using samples from three mini outbreaks-2007-2009 in Pakistan”. BMC Microbiology 1 (2011): 1-8.
    11. Malayan J., et al. “Anti-mumps virus activity by extracts of Mimosa pudica, a unique Indian medicinal plant”. Indian Journal of Virology2 (2013): 166-173.
    12. Dereeper A., et al. “BLAST‐EXPLORER helps you building datasets for phylogenetic analysis”. BMC Evology and Biology1 (2010): 8.
    13. Kumar S., et al. “Molecular evolutionary genetics analysis across computing platforms”. Molecular Biology and Evolution 35 (2018): 1547‐1549.
    14. Dang TT., et al. “Whole genome sequencing and genetic variations in several dengue virus type 1 strains from unusual dengue epidemic of 2017 in Vietnam”. Virology Journal1 (2020): 1-10.
    15. Collins ND., et al. “Structural and Nonstructural Genes Contribute to the Genetic Diversity of RNA Viruses”. MBio5 (2018): e01871-18.
    16. Azhar EI., et al. “Complete genome sequencing and phylogenetic analysis of dengue type 1 virus isolated from Jeddah, Saudi Arabia”. Virology Journal 12 (2015): 1.
    17. Rico-Hesse R. “Microevolution and virulence of dengue viruses”. Advances in Virus Research 59 (2003): 315-341.
    18. Martina BE., et al. “Dengue virus pathogenesis: an integrated view”. Clinical Microbiology Review 4 (2009): 564-581.
    19. Sessions OM., et al. “Analysis of dengue virus genetic diversity during human and mosquito infection reveals genetic constraints”. PLOS Neglected Tropical Diseases9 (2015): e0004044.
    20. Damodar T., et al. “Clinical and laboratory profile of dengue viral infections in and around Mangalore, India”. Indian Journal of Medical Microbiology2 (2017): 256-261.
    21. Halsey ES., et al. “Correlation of serotype specific dengue virus infection with clinical manifestations”. PLOS Neglected Tropical Diseases5 (2012): e1638.
    22. Ahlawat RS and Kalra T. “Atypical manifestations of dengue fever in a recent dengue outbreak”. Annals of Tropical Medicine and Public Health 10 (2017): 1448-1452.
    23. Soo KM., et al. “Meta-analysis of dengue severity during infection by different dengue virus serotypes in primary and secondary infections”. PloS One5 (2016): e0154760.
    24. Anantapreecha S., et al. “Serological and virological features of dengue fever and dengue haemorrhagic fever in Thailand from 1999 to 2002”. Epidemiology Infectious3 (2005): 503-507.
    25. Cecilia D., et al. “Emergence of the Asian genotype of DENV-1 in South India”. Virology 510 (2017): 40-45.
    26. Lee KS., et al. “Dengue virus surveillance in Singapore reveals high viral diversity through multiple introductions and in situ evolution”. Infection, Genetics and Evolution 1 (2012): 77-85.
    27. Waman VP., et al. “Analysis of genotype diversity and evolution of Dengue virus serotype 2 using complete genomes”. Peer Journal 4 (2016): e2326.
    28. Anoop M., et al. “Genetic characterization of dengue virus serotypes causing concurrent infection in an outbreak in Ernakulam, Kerala, South India”. Indian Journal of Experimental Biology 48 (2010): 849-857.
    29. Vaddadi K., et al. “Complete genome characterization and evolutionary analysis of serotype-4 associated with severe dengue”. Epidemiology Infectious7 (2017): 1443-1450.
    30. Uehara A., et al. “Analysis of Dengue Serotype 4 in Sri Lanka during the 2012-2013 Dengue Epidemic”. American Journal of Tropical Medicine and Hygiene 97 (2017): 130-136.

    Citation

    Citation: Kamalraj Mohan.,et al. “Molecular Characterization of Dengue Viruses Causing Hemorrhagic Fever from the Atypical Dengue Cases in Chennai, India".Acta Scientific Microbiology 7.3 (2024): 31-40.

    Copyright

    Copyright: © 2024 Jeevan Malaiyan., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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