Sunita R Borkar^1,2* and Saroj N. Bhosle¹

¹Department of Microbiology, Goa University, Taleigao Plateau, Goa, India
²UG and PG Department of Microbiology, P.E.S’s R.S.N College of Arts and Science, Farmagudi, Ponda, Goa, India

*Corresponding Author: Sunita R Borkar, Associate Professor in Microbiology, Department of Microbiology, Goa University, Taleigao Plateau, Goa, India.

Received: December 11, 2023; Published: January 16, 2024

Abstract

Alkali-stable amylases offer greater advantages over other amylases in biotechnological applications. In the present study, we aimed to produce, optimize and characterize amylase from alkaliphilic strains SB-D and SB-W isolated from sediment samples of an agrochemical factory. Both the strains were identified as Alkalihalobacillus sp. by 16S rRNA sequencing. The strain SB-D efficiently hydrolyzed 1% starch with optimized conditions of 2% inoculum, pH 10.3, 25°C, at 200 rpm after 24 h, while for SB-W it was 2% inoculum, pH 10.3, 55°C, at 250 rpm and after 16 h. Further, crude amylases from strain SB-D and SB-W were partially purified to 2.9 and 1.8 fold, respectively by ammonium sulfate precipitation method and showed multiple protein bands on SDS-PAGE. Interestingly, amylase from SB-W retained 80% activity in the presence of butanol, isopropanol and ether and showed activity at a broad pH range of 7.3-11.3 and temperature of 25˚C -75˚C. In comparison with commercial α-amylase; amylase from both strains showed a similar pattern of reducing sugar and iodine blue value. Furthermore, amylases required metal ions for their activity and were stable in the presence of detergent additives. Conclusively, SB-D and SB-W are potential producers of thermo-alkali tolerant and detergent-stable amylases of biotechnological significance.

Keywords: Agrochemical Factory; Alkalihalobacillus sp. Strain SB-D; Alkalihalobacillus sp. Strain SB-W; Alkali Stable

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Citation

Citation: Sunita R Borkar and Saroj N. Bhosle. “Production, Optimization, and Partial Characterization of Alkali-stable Alpha-Amylases from Alkalihalobacillus Isolates".Acta Scientific Microbiology 7.2 (2024): 42-53.

Copyright

Copyright: © 2024 Sunita R Borkar and Saroj N. Bhosle. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.