Hemavati Ranebennur¹*, Basavaraj YB², Nayan Deepak Gangappa³, Kirti Rawat¹, Manoj Kumar Yadav⁴ and Kartar Singh⁵

¹Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India
²Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, Regional Station, Pune Maharashtra, India
³Fruit Science, Central Horticultural Experiment Station – Chettalli, Kodagu, India
⁴Plant pathology, ICAR- Indian Agricultural Research Institute, Regional Station Karnal, Haryana, India
⁵ICAR-National Bureau of Plant Genetic Resources, Regional Station Jodhpur, Rajasthan, India

*Corresponding Author: Hemavati Ranebennur, Division of Plant Pathology, ICAR-Indian Agricultural Research Institute, New Delhi, India.

Received: November 28, 2023; Published: January 08, 2024

Abstract

Suspected phytoplasma symptoms of flat stem, witches’-broom, little leaf, and apical fasciation were observed on Clarkia unguiculata plants in March, 2022 at the Division of Plant Pathology, IARI. The plant samples (both symptomatic and asymptomatic) and leafhopper species were collected and subjected to nested PCR assays using universal primer pairs (P1/P7 followed by R16F2n/R2). All of the symptomatic samples yielded PCR amplicons of the expected 1.2 kb size for nested reaction. The only leafhopper species identified was Hishimonus phycitis, which likewise tested positive in PCR assays with the anticipated amplicon of 1.2 kb using the same set of phytoplasma specific primers as used for the plant samples. For further confirmation a multilocus gene, secA gene (480bp) was also amplified using universal primers. Nucleotide sequence identities of 100% with 16S rRNA and 100% with secA gene was found with strains of aster yellows group (16SrI-B). This is the first report of the phytoplasma association with Clarkia unguiculata in the world.

Keywords: Ornamental; Multilocus Gene; secA; 16S rRNA; North India

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Citation

Citation: Hemavati Ranebennur., et al. “Clarkia unguiculata: A New Plant Host of Candidatus Phytoplasma Asteris (16SrI-B) Related Strain".Acta Scientific Microbiology 7.2 (2024): 21-27.

Copyright

Copyright: © 2024 Hemavati Ranebennur., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.