Sabita Tamang¹, Ruby Maharjan¹, Amin Maharjan¹ and Sagar Atri²*

¹Department of Biotechnology, Kantipur Valley College, Lalitpur, Nepal ²National Ayurveda Research and Training Centre, Kritipur, Nepal

*Corresponding Author: Sagar Atri, National Ayurveda Research and Training Centre, Kritipur, Nepal.

Received: July 24, 2023; Published: December 02, 2023

Abstract

Traditional medicine and the use of indigenous medicinal plants have been an integral part of the healthcare system and cultural practices in Nepal for generations. However, there has been a lack of systematic research on their antioxidant and antimicrobial properties. Our study focused on evaluating the medicinal potential of two indigenous plants of Nepal; T. sinensis and B. aristata. The plant samples were collected from different ecological regions in Nepal, specifically T. sinensis and B. aristata, from altitudes of 1161 and 1666m, respectively, in the hills of Dhunibesi-7, Dhading, Nepal. Crude methanolic extracts were prepared from these plants using the Soxhlet apparatus method and concentrated through rotary vacuum evaporation. Preliminary phytochemical analysis revealed the presence of important compounds such as alkaloids, carbohydrates, tannins, flavonoids, saponins, resins, proteins, amino acids, and fatty acids. Antimicrobial efficacy was assessed using Agar-disc diffusion against gram-positive (Bacillus subtilis, Staphylococcus aureus) and gram-negative (Klebsiella pneumoniae, Enterobacter) bacteria. All extracts exhibited antimicrobial activity, with B. aristata leaves being most effective against B. subtilis. The antioxidant potential was evaluated using the 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, with B. aristata stem extract showing the strongest activity (IC50: 82.101 μg/ml) and T. sinensis leaf extract showing the lowest (IC50: 128.53 μg/ml). Notably, both B. aristata stem and T. sinensis leaves demonstrated superior antioxidant performance. In conclusion, T. sinensis and B. aristata emerge as promising sources of medicinal compounds. Their diverse phytochemical composition likely contributes to their observed antimicrobial and antioxidant properties. This study emphasizes the value of these indigenous plants in Nepal's traditional medicine and highlights their potential as sources of medicinal and antioxidant agents.

Keywords: Anti-microbial Activity; Anti-oxidant Activity; Phytochemicals; Zone of Inhibition; Half Maximal Inhibitory Concentration (IC50)

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Citation

Citation: Sabita Tamang., et al. “An Assessment of Phytochemical, Anti-microbial, and Anti-oxidant Properties of Tinospora sinensis and Berberis aristat of Nepal".Acta Scientific Microbiology 4.6 (2023): 03-11.

Copyright

Copyright: © 2023 Sagar Atri., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.