Ndaleh Wozerou Nghonjuyi*

Department of Animal Science, University of Buea/Saint Monica University Higher Institute, Cameroon

*Corresponding Author: Ndaleh Wozerou Nghonjuyi, Department of Animal Science, University of Buea/Saint Monica University Higher Institute, Cameroon.

Received: October 25, 2023; Published: November 19, 2023

Abstract

Escherichia coli and Klebsiella species are pathogens of significant public health interest to which new antibiotics therapies are urgently needed, where they have extended Spectrum of Beta-Lactamase (ESBL) activity. This study was aimed to determine the prevalence of ESBL-production among the environmental isolates of E. coli and Klebsiella species and their susceptibility to seven antimicrobials at the Buea Regional Hospital (BRH). Samples from surfaces of doors, toilet and hospital beds and tables were collected by swabbing, using sterile hydrophilic cotton swabs and inoculated on EMB. Environmental isolates were tested for ESBL production by using the double disk synergy test by Kirby-Bauer disc diffusion method on Muller-Hinton agar. Susceptibility to antibiotics was performed using the disc diffusion method according to the Clinical Laboratory Standards Institute guidelines. ESBL was observed in 2 isolates giving an overall prevalence of 12.5% (2/16), these two ESBL isolates were Klebsiella species. For susceptibility profile, cefepime and cefixime were 100% active against ESBL producing strains. Four Klebsiella (33.3%) isolates showed resistance to all the antibiotics tested. The study indicates low prevalence of ESBL producing Klebsiella species and E. coli from the environment in BRH. However, it gives insight to a foreseeable problem giving the increasing incidence of antibiotics abuse.

Keywords: Antimicrobials; Bet-lactamase; Buea; Cameroon; Hospital Surfaces; Klebsiella

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Citation

Citation: Ndaleh Wozerou Nghonjuyi. “Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella Species from Buea Regional Hospital Surfaces and Their Susceptibility Pattern to Antimicrobials in Cameroon".Acta Scientific Microbiology 6.12 (2023): 28-38.

Copyright

Copyright: © 2023 Ndaleh Wozerou Nghonjuyi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.