Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 12

Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella Species from Buea Regional Hospital Surfaces and Their Susceptibility Pattern to Antimicrobials in Cameroon

Ndaleh Wozerou Nghonjuyi*

Department of Animal Science, University of Buea/Saint Monica University Higher Institute, Cameroon

*Corresponding Author: Ndaleh Wozerou Nghonjuyi, Department of Animal Science, University of Buea/Saint Monica University Higher Institute, Cameroon.

Received: October 25, 2023; Published: November 19, 2023

Abstract

Escherichia coli and Klebsiella species are pathogens of significant public health interest to which new antibiotics therapies are urgently needed, where they have extended Spectrum of Beta-Lactamase (ESBL) activity. This study was aimed to determine the prevalence of ESBL-production among the environmental isolates of E. coli and Klebsiella species and their susceptibility to seven antimicrobials at the Buea Regional Hospital (BRH). Samples from surfaces of doors, toilet and hospital beds and tables were collected by swabbing, using sterile hydrophilic cotton swabs and inoculated on EMB. Environmental isolates were tested for ESBL production by using the double disk synergy test by Kirby-Bauer disc diffusion method on Muller-Hinton agar. Susceptibility to antibiotics was performed using the disc diffusion method according to the Clinical Laboratory Standards Institute guidelines. ESBL was observed in 2 isolates giving an overall prevalence of 12.5% (2/16), these two ESBL isolates were Klebsiella species. For susceptibility profile, cefepime and cefixime were 100% active against ESBL producing strains. Four Klebsiella (33.3%) isolates showed resistance to all the antibiotics tested. The study indicates low prevalence of ESBL producing Klebsiella species and E. coli from the environment in BRH. However, it gives insight to a foreseeable problem giving the increasing incidence of antibiotics abuse.

Keywords: Antimicrobials; Bet-lactamase; Buea; Cameroon; Hospital Surfaces; Klebsiella

References

  1. Najjuka CF., et al. “Antimicrobial susceptibility profiles of Escherichia coli and Klebsiella pneumoniae isolated from outpatients in urban and rural districts of Uganda”. BMC Research Notes 9 (2016): 1-4.
  2. Andrew B., et al. “Prevalence of extended-spectrum beta-lactamases-producing microorganisms in patients admitted at KRRH, Southwestern Uganda”. International Journal of Microbiology (2017).
  3. Shaikh S., et al. “Risk factors for acquisition of extended spectrum beta lactamase producing Escherichia coli and Klebsiella pneumoniae in North-Indian hospitals”. Saudi Journal of Biological Sciences1 (2015): 37-41.
  4. Gundogan N and Avci E. “Prevalence and antibiotic resistance of extended-spectrum beta-lactamase (ESBL) producing Escherichia coli and Klebsiella species isolated from foods of animal origin in Turkey”. African Journal of Microbiology Research31 (2013): 4059-4064.
  5. Riaz S., et al. “Prevalence and comparison of Beta-lactamase producing Escherichia coli and Klebsiella spp from clinical and environmental sources in Lahore, Pakistan”. African Journal of Microbiology Research2 (2012): 465-470.
  6. Sherchan JB., et al. “Clinical epidemiology and molecular analysis of extended-spectrum-β-lactamase-producing Escherichia coli in Nepal: characteristics of sequence types 131 and 648”. Antimicrobial Agents and Chemotherapy6 (2015): 3424-3432.
  7. Voor in ‘t holt AF., et al. “Detection of healthcare-related extended-spectrum beta-lactamase-producing Escherichia coli transmission events using combined genetic and phenotypic epidemiology”. PLoS One7 (2016): e0160156.
  8. Rubio-Perez I., et al. “Extended-spectrum beta-lactamase-producing bacteria in a tertiary care hospital in Madrid: epidemiology, risk factors and antimicrobial susceptibility patterns”. Emerging Health Threats Journal 1 (2012): 11589.
  9. Bakkali M., et al. “Characterization of bacterial strains and their resistance status in hospital environment”. Journal of Tropical Diseases 180 (2015): 2.
  10. Mehrad B., et al. “Antimicrobial resistance in hospital-acquired gram-negative bacterial infections”. Chest5 (2015): 1413-1421.
  11. Tetteh FK. “Extended spectrum beta-lactamase in clinical isolates of Escherichia coli and Klebsiella pneumoniae from the Tamale Teaching Hospital (Doctoral dissertation, MA Thesis, College of Health Sciences, University of Ghana)”.
  12. Magoué CL., et al. “Prevalence and spread of extended-spectrum β-lactamase-producing Enterobacteriaceae in Ngaoundere, Cameroon”. Clinical Microbiology and Infection9 (2013): E416-420.
  13. CLSI, Performance Standards for Antimicrobial Susceptibility Testing: CLSI Supplement M100S, Clinical and Laboratory Standards Institute, Wayne, PA, USA (2016).
  14. Agbo EC. “Occurence of Beta-Lactamases in Escherichia Coli and Klebsiella Species isolated from Environmental Sources and Hospital Patients in Nsukka, Enugu State (Doctoral dissertation)”.
  15. Muzslay M., et al. “ESBL-producing Gram-negative organisms in the healthcare environment as a source of genetic material for resistance in human infections”. Journal of Hospital Infection1 (2017): 59-64.
  16. Engda T., et al. “Prevalence and antimicrobial susceptibility patterns of extended spectrum beta-lactamase producing Entrobacteriaceae in the University of Gondar Referral Hospital environments, northwest Ethiopia”. BMC Research Notes1 (2018): 1-7.
  17. Das S., et al. “Antimicrobial Susceptibility Pattern of Pseudomonas aeruginosa with Special Reference to ESBL Producers from Various Clinical Samples at a Tertiary Care Center in Bihar”. International Journal of Research and Review1 (2020).
  18. Afifi MM. “Detection of extended spectrum betalactamase producing Klebsiella pneumoniae and Escherichia coli of environmental surfaces at upper Egypt”. International Journal of Biological Chemistry2 (2013): 58-68.

Citation

Citation: Ndaleh Wozerou Nghonjuyi. “Prevalence of Extended Spectrum Beta Lactamase (ESBL) Producing Escherichia coli and Klebsiella Species from Buea Regional Hospital Surfaces and Their Susceptibility Pattern to Antimicrobials in Cameroon".Acta Scientific Microbiology 6.12 (2023): 28-38.

Copyright

Copyright: © 2023 Ndaleh Wozerou Nghonjuyi. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




Metrics

Acceptance rate30%
Acceptance to publication20-30 days

Indexed In






News and Events


Contact US