Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 9

Biochemical and Antimicrobial activity of Potential Probiotics Isolated from Halari Milk

Ankur Kumari1, Anuradha Bhardwaj2*, Parvati Sharma1*, Yash Pal2, Varij Nayan3, Prashant Singh2 and Ashish Sindhu1

1Department of Zoology, Chaudhary Bansi Lal University, Bhiwani, Haryana, India

2National Research Centre on Equine, Hisar, India

3Central Institute for Research on Buffaloes, Hisar, India

*Corresponding Author: Parvati Sharma and Anuradha Bhardwaj, Department of Zoology, Chaudhary Bansi Lal University, Bhiwani, Haryana and National Research Centre on Equine, Hisar India.

Received: August 07, 2023; Published: August 18, 2023

Abstract

The probiotic-enriched food product that not only satisfies nutritional needs but also boost the immune system, is now appalling situation. Non bovine milk, contains various bioactive components which are potentially involved in the immune system functioning, bone strengthening and for balancing gut flora with reliable probiotics. Among these non-bovine milk sources, therapeutic properties of Donkey milk are researched since antiquity, but the microbiological profile has gotten less attention. Therefore, current study emphasized the significance, isolation, biochemical identity, and antimicrobial efficacy of probiotics found in donkey milk. A total of 35 isolates were collected from donkey milk samples and eight potent isolates were chosen based on screening and further evaluated for their significant probiotic activity like, resistance for acidic conditions, high bile salt concentration, antibiotic susceptibility and antibacterial activity against pathogenic bacteria of these isolates was examined. Lactococcus lactis subsp cremoris and Enterococcus faceium are found to resistant against in vitro stress conditions and exhibited maximum antimicrobial activity against Staphylococcus aureus, Pseudomonas and Bacillus subtilis strains. In line with the findings of the present study, these potent DM probiotics can be employed as bio preservatives to prevent the proliferation of pathogenic or food-spoiled bacteria.

 Keywords: Donkey Milk; Probiotics; Bacteria; Biochemical Test; Antimicrobial Activity

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Citation

Citation: Parvati Sharma and Anuradha Bhardwaj., et al. “Biochemical and Antimicrobial activity of Potential Probiotics Isolated from Halari Milk". Acta Scientific Microbiology 6.9 (2023): 73-83.

Copyright

Copyright: © 2023 Parvati Sharma and Anuradha Bhardwaj., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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