Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 9

Biochemical and Antimicrobial activity of Potential Probiotics Isolated from Halari Milk

Ankur Kumari1, Anuradha Bhardwaj2*, Parvati Sharma1*, Yash Pal2, Varij Nayan3, Prashant Singh2 and Ashish Sindhu1

1Department of Zoology, Chaudhary Bansi Lal University, Bhiwani, Haryana, India

2National Research Centre on Equine, Hisar, India

3Central Institute for Research on Buffaloes, Hisar, India

*Corresponding Author: Parvati Sharma and Anuradha Bhardwaj, Department of Zoology, Chaudhary Bansi Lal University, Bhiwani, Haryana and National Research Centre on Equine, Hisar India.

Received: August 07, 2023; Published: August 18, 2023


The probiotic-enriched food product that not only satisfies nutritional needs but also boost the immune system, is now appalling situation. Non bovine milk, contains various bioactive components which are potentially involved in the immune system functioning, bone strengthening and for balancing gut flora with reliable probiotics. Among these non-bovine milk sources, therapeutic properties of Donkey milk are researched since antiquity, but the microbiological profile has gotten less attention. Therefore, current study emphasized the significance, isolation, biochemical identity, and antimicrobial efficacy of probiotics found in donkey milk. A total of 35 isolates were collected from donkey milk samples and eight potent isolates were chosen based on screening and further evaluated for their significant probiotic activity like, resistance for acidic conditions, high bile salt concentration, antibiotic susceptibility and antibacterial activity against pathogenic bacteria of these isolates was examined. Lactococcus lactis subsp cremoris and Enterococcus faceium are found to resistant against in vitro stress conditions and exhibited maximum antimicrobial activity against Staphylococcus aureus, Pseudomonas and Bacillus subtilis strains. In line with the findings of the present study, these potent DM probiotics can be employed as bio preservatives to prevent the proliferation of pathogenic or food-spoiled bacteria.

 Keywords: Donkey Milk; Probiotics; Bacteria; Biochemical Test; Antimicrobial Activity


  1. Hemming F. “Usage Of 17 Specific Names Based on Wild Species Which Are Pre-Dated by Or Contemporary with those Based on Domestic Animals (Lepidoptera, Osteichthyes, Mammalia): Conserved”. The Bulletin of Zoological Nomenclature 60 (2003): 81-84.
  2. Grubb P. “Order Perissodactyla. In Wilson, D.E.; Reeder, D.M. Mammal Species of the World: A Taxonomic and Geographic Reference” (3rd edition) (2005): 62265494.
  3. Garhwal R., et al. “Systematic Review of the Bioactive Components, Nutritional Qualities and Potential Therapeutic Applications of Donkey Milk”. Journal of Equine Veterinary Science 115 (2022): 104006.
  4. Press information bureau, Ministry of Fisheries, Animal Husbandry and Dairying, Government of India. Saurashtra Maldhari Sammelan (2022).
  5. ISO EN. "15214. Microbiology of Food and Animal Feeding Stuffs-Horizontal Method for the Enumeration of Mesophilic Lactic Acid Bacteria-Colony-Count Technique at 30 Degrees C. In ICS, 7 (1998): 300.
  6. Rastogi S., et al. “In vitro evaluation of probiotic potential and safety assessment of Lactobacillus mucosae strains isolated from Donkey’s lactation”. Probiotics and Antimicrobial Proteins 12 (2019): 1045-1056.
  7. Cirrincione S., et al. “Donkey Milk Fermentation by Lactococcus lactis subsp. Cremoris and Lactobacillus rhamnosus Affects the Antiviral and Antibacterial Milk Properties”. Molecules16 (2021): 5100.
  8. Bhardwaj A., et al. “Donkey milk composition and its therapeutic applications”. Indian Journal of Animal Research 6 (2020): 837-840.
  9. Kaushik JK., et al. “Functional and probiotic attributes of an indigenous isolate of Lactobacillus plantarum”. PLoS One 4 (2009): e8099.
  10. Massouras T., et al. “Microbial Profile Antibacterial Properties and Chemical Composition of Raw Donkey Milk”. Animals 10 (2020): 2001.
  11. Sharma P., et al. “Isolation and identification of pathogenic bacteria and fungi isolated from skin ulcers of Cirrhinus mrigala”. Indian Journal of Animal Research 4 (2013): 283-291.
  12. Sharma P., et al. “Relative efficacy of two probiotics in controlling the epizootic ulcerative syndrome disease in Mrigal (Cirrhinus mrigala)”. Journal of Fisheries and Aquatic Science 8.2 (2013): 305.
  13. Garrity and Jones D. “Bergey's Manual of Systematic Bacteriology”. New York: Springer 3 (2009).
  14. Kleerebezem M and Hugenholtz J. “Metabolic pathway engineering in lactic acid bacteria”. Current Opinion in Biotechnology 14 (2003): 232-237.
  15. Zhang B., et al. “Screening of probiotic activities of lactobacilli strains isolated from traditional Tibetan Qula, a raw yak milk cheese. Asian-australas”. Journal of Animal Science 10 (2016): 1490-1499.
  16. Vlková E., et al. “Antimicrobial susceptibility of bifidobacteria isolated from gastrointestinal tract of calves”. Livestock Science 105 (2016): 253-259.
  17. Aspri M., et al. “Raw donkey milk as a source of Enterococcus diversity: Assessment of their technological properties and safety characteristics”. Food Control (2016).
  18. Cavallarin L., et al. “A survey on the milk chemical and microbiological quality in dairy donkey farms located in North-Western Italy”. Food Control 50 (2015): 230–235.
  19. Neviani E., et al. “Characterization of Lactobacillus helveticus strains resistant to lysozyme”. Le Lait 71 (1991): 65-73.
  20. Clinical and Laboratory Standards Institute (CLSI). “Performance standards for antimicrobial susceptibility testing: twenty-second informational supplement. In: CLSI document M100-S22. Clinical Laboratory Standard Institute, Wayne (2015).
  21. Carminati D., et al. “Biotyping of cultivable lactic acid bacteria isolated from donkey milk”. Applied Microbiology 3 (2014): 299-305.
  22. Quigley L., et al. “The complex microbiota of raw milk”. FEMS Microbiology Reviews 37 (2013): 664–698.
  23. Kumari A and Sharma P. “The Nutraceutical Characteristics of Donkey Milk: A New Insight for its Potential Probiotics”. Journal of Veterinary Medicine and Animal Sciences 2 (2021): 1074.
  24. Kumari A., et al. “Exploration and Insights of Potential Probiotics Diversity of Donkey (Equus africanus asinus) Milk. 2nd International Electronic Conference on Diversity”. IECD (2022).
  25. Kumari A., et al. “Isolation and identification of probiotic bacteria from donkey (Equus africanus asinus) milk of South-Western Haryana”. International Journal of Innovative Science, Engineering and Technology 4 (2022): 2348-7968.
  26. Papademas P., et al. “Probiotic, Antimicrobial, Antioxidant and Sensory properties of Fermented donkey milk with Lactobacillus fermentum ME-3 and Lactobacillus acidophilus (ATCC4356)”. BAOJ Microbiology 1 (2015): 004.
  27. Gupta AK., et al. “Phenotypic Clustering of Indian Donkey Population Belonging to Six Agro Climatic Regions”. Journal of Biodiversity and Endangered Species 5 (2017): 201.
  28. Gupta AK., et al. “Biochemical profiles of Indian donkey population located in six different agro-climatic zones”. Comparative Clinical Pathology (2015).
  29. Jagadeesh KS. “Lactic acid bacteria as a source of functional ingredients”. South Indian Journal of Biological Sciences 1 (2015): 70-71.
  30. Bhardwaj A., et al. “Donkey milk composition and its therapeutic applications”. Indian Journal of Animal Research 6 (2020): 837-840.


Citation: Parvati Sharma and Anuradha Bhardwaj., et al. “Biochemical and Antimicrobial activity of Potential Probiotics Isolated from Halari Milk". Acta Scientific Microbiology 6.9 (2023): 73-83.


Copyright: © 2023 Parvati Sharma and Anuradha Bhardwaj., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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