Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 8

Cross-sectional Study on Bovine Tuberculosis Based on Post Mortem and Bacteriological Examination in Four Abattoirs of Northern Algeria: Slaughterhouses as Source of Data for Disease Control

Hanane Damene1, Razika Boukert1, Naima Bouzid2, Yassine Zerizer2, Ali Berber1, Naima Sahraoui1 and Djamel Tahir1,3*

1Institute of Veterinary Sciences, University Blida 1, Blida, Algeria

2Directory of Agricultural Services, Béjaia, Algeria

3Institute of Pasteur, Paris, France

*Corresponding Author: Djamel Tahir, Institute of Veterinary Sciences, University Blida 1, Blida, Algeria.

Received: July 17, 2023; Published: July 25, 2023


Bovine tuberculosis (BTB) remains a major zoonotic disease, causing economic losses as well as public health problems, particularly in developing countries. The aim of this study was to determine the prevalence of BTB and the associated risk factors in northern Algeria, based on the cattle population slaughtered in four abattoirs. Overall, 3,546 slaughtered cattle were inspected for detection of visible tuberculosis-like lesions between 2017 and 2019. Furthermore, tissue samples were collected from the affected carcasses (n = 232) and screened using the Ziehl–Neelsen (ZN) staining technique and mycobacterial culture. Visible tuberculosis-like lesions occurred in 6.5% (232/3,546) of the slaughtered animals. Whatever the technique used, the prevalence of BTB disease was found to be statistically associated with age, sex, and breed (p<0.0001). The thoracic lymph nodes including the tracheobronchial and mediastinal LN were mostly affected, at rates of 52.6% and 31.9%, respectively. The majority (69.4%; n = 161) of granulomas found in the thoracic lymph nodes were characterized by caseous necrosis (34%) or caseous-calcified lesions (35.3%). The present study demonstrated that despite the efforts of authorities to eradicate BTB, this disease is still enzootic in Algeria, since the prevalence remains important. Finally, our findings highlighted the importance of abattoirs as a source of information for recording the BTB epidemiological situation in a given area, contributing to updating the design and implementation of control and prevention strategies.

 Keywords: Bovine Tuberculosis; Cattle; Risk Factors; Post Mortem Inspection; Bacteriological Examination; Algeria


  1. Humblet MF., et al. “Classification of worldwide bovine tuberculosis risk factors in cattle: a stratifiedapproach”. Veterinary Research5 (2009): 1-24.
  2. Cvetnic Z., et al. “Mycobacterium caprae in cattle and humans in Croatia”. The International Journal of Tuberculosis and Lung Disease6 (2007): 652-658.
  3. Sulieman MS., et al. “Identification of acid fast bacteria from caseous lesions in cattle in Sudan”. Journal of Veterinary Medicine, Series B9 (2002): 415-418.
  4. Michelet L., et al. “Mycobacterium microti interferes with bovine tuberculosis surveillance”. Microorganisms12 (2020): 1850.
  5. Thoen CO., et al. “Mycobacterium bovis infection in animals and humans”. John Wiley and Sons 2 (2008): 1-329.
  6. O’Reilly LM., et al. “The epidemiology of Mycobacterium bovis infections in animals and man: a review”. Tubercle and Lung Disease 76 (1995): 1-46.
  7. Grange JM. “Mycobacterium bovis infection in human beings”. Tuberculosis1-2 (2001): 71-77.
  8. De Kantor IN., et al. “An update on bovine tuberculosis programmes in Latin American and Caribbean countries”. Veterinary Microbiology 112.2-4 (2006): 111-118.
  9. Domingo M., et al. “Pathology of bovine tuberculosis”. Research in Veterinary Science 97 (2014): S20-S29.
  10. Corner LA., et al. “Efficiency of inspection procedures for the detection of tuberculous lesions in cattle”. Australian Veterinary Journal11 (1990): 389-392.
  11. Wangoo A., et al. “Advanced granulomatous lesions in Mycobacterium bovis-infected cattle are associated with increased expression of type I procollagen, γδ (WC1+) T cells and CD 68+ cells”. Journal of Comparative Pathology4 (2005): 223-234.
  12. Vordermeier HM., et al. “Correlation of ESAT-6-specific gamma interferon production with pathology in cattle following Mycobacterium bovis BCG vaccination against experimental bovine tuberculosis”. Infection and Immunity6 (2002): 3026-3032.
  13. Palmer MV., et al. “Lesion development and immunohistochemical changes in granulomas from cattle experimentally infected with Mycobacterium bovis”. Veterinary Pathology6 (2007): 863-874.
  14. Carrisoza-Urbina J., et al. “Atypical granuloma formation in Mycobacterium bovis-infected calves”. PLoS One7 (2019): e0218547.
  15. Acha PN., et al. “Zoonoses and Communicable Diseases Common to Man and Animals. Bacteriose and mycoses”. Scientific and Technical Publication (DC: Pan American Health Organization, Washington) 3 (2001): 283-299.
  16. Word Organisation for Animal Health (OIE). “Contronlling of bovine tuberculosis: a one health chalenge”. Bulletin (2019): 2-82.
  17. Demelash B., et al. “Prevalence of bovine tuberculosis in Ethiopian slaughter cattle based on post-mortem examination”. Tropical Animal Health and Production5 (2009): 755-765.
  18. Sahraoui N., et al. “Molecular characterization of Mycobacterium bovis strains isolated from cattle slaughtered at two abattoirs in Algeria”. BMC Veterinary Research 1 (2009): 4.
  19. Damene H., et al. “Broad diversity of Mycobacterium tuberculosis complex strains isolated from humans and cattle in Northern Algeria suggests a zoonotic transmission cycle”. PLoS Neglected Tropical Diseases 11 (2020): e0008894.
  20. Word Organisation for Animal Health (OIE). “Bovine tuberculosis:The tuberculin test Manual of diagnostic tests and vaccines for terrestrial animals”. OIE Terrestrial Manual 6 (2009): 6-7.
  21. World Health Organization (WHO). “Laboratory services in tuberculosis control. Geneva Switzerland: Part II: Microscopy”. World Health Organization (1998): 1-63.
  22. Corner LA. “Post mortem diagnosis of Mycobacterium bovis infection in cattle”. Veterinary Microbiology 40.1-2 (1994): 53-63.
  23. Shitaye JE., et al. “A prevalence study of bovine tuberculosis by using abattoir meat inspection and tuberculin skin testing data, histopathological and IS6110 PCR examination of tissues with tuberculous lesions in cattle in Ethiopia”. Veterinarni Medicina 11 (2006): 512.
  24. Olea-Popelka FJ., et al. “Risk factors for disclosure of additional tuberculous cattle in attested-clear herds that had one animal with a confirmed lesion of tuberculosis at slaughter during 2003 in Ireland”. Preventive Veterinary Medecine 85 1-2 (2008): 81-91.
  25. Shittu A., et al. “Factors associated with bovine tuberculosis confirmation rates in suspect lesions found in cattle at routine slaughter in Great Britain, 2003-2008”. Preventive Veterinary Medecine3-4 (2013): 395-404.
  26. Kasaye S., et al. “Prevalence of camel tuberculosis at Akaki abattoir in Addis Ababa, Ethiopia”. African Journal of Microbiology Research 20 (2013): 2184-9.
  27. Pollock JM., et al. “Mycobacterium bovis infection and tuberculosis in cattle”. The Veterinary Journal 2 (2002): 115-127.
  28. Boukary AR., et al. “Risk factors associated with bovine tuberculosis and molecular characterization of Mycobacterium bovis strains in urban settings in Niger”. Transboundary and Emerging Diseases 6 (2012): 490-502.
  29. Habarugira G., et al. “Bovine tuberculosis in Rwanda: Prevalence and economic impact evaluation by meat inspection at Société des Abattoirs de Nyabugogo-Nyabugogo Abattoir, Kigali”. Journal of the South African Veterinary Association 1 (2014): 1-5.
  30. Elias K., et al. “Status of bovine tuberculosis in Addis Ababa dairy farms”. Revue scientifique et technique 3 (2008): 915.
  31. Skuce RA., et al. “Bovine tuberculosis (TB): a review of cattle-to-cattle transmission, risk factors and susceptibility”. Bacteriology Branch Veterinary Sciences Division Agrifood and Biosciences Institute (2011).
  32. Teklu A., et al. “Tuberculous lesions not detected by routine abattoir inspection: the experience of the Hossana municipal abattoir, southern Ethiopia”. Revue Scientifique et Technique-Office International Des Epizooties 3 (2004): 957-964.
  33. Liebana E., et al. “Pathology of naturally occurring bovine tuberculosis in England and Wales”. Veterinary Journal3 (2008): 354-360.
  34. Proaño-Pérez F., et al. “Post-mortem examination and laboratory-based analysis for the diagnosis of bovine tuberculosis among dairy cattle in Ecuador”. Preventive Veterinary Medecine1-2 (2011): 65-72.
  35. Converse PJ., et al. “Cavitary tuberculosis produced in rabbits by aerosolized virulent tubercle bacilli”. Infection and Immunity11 (1996): 4776-4787.
  36. Menin Á., et al. “Asymptomatic cattle naturally infected with Mycobacterium bovis present exacerbated tissue pathology and bacterial dissemination”. PloS One1 (2013): e53884.
  37. AVSEVER ML., et al. “New spoligotyping pattern of Mycobacterium bovis isolates from farm animals in Turkey”. Ankara Üniversitesi Veteriner Fakültesi Dergisi 1 (2017): 37-43.
  38. De la Rua-Domenech R., et al. “Ante mortem diagnosis of tuberculosis in cattle: a review of the tuberculin tests, γ-interferon assay and other ancillary diagnostic techniques”. Research Veterinary Science2 (2006): 190-210.


Citation: Hanane Damene., et al. “Cross-sectional Study on Bovine Tuberculosis Based on Post Mortem and Bacteriological Examination in Four Abattoirs of Northern Algeria: Slaughterhouses as Source of Data for Disease Control". Acta Scientific Microbiology 6.8 (2023): 49-46.


Copyright: © 2023 Hanane Damene., et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


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