Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 7

Effect of Dimethyl Sulfoxide on the Bioluminescence Expression of the Inducible SOS-Lux gene Biosensor E. coli C600 pPSL-1 and E. coli DPD1718 elicited by Mitomycin-C

Awulu EA1,2*

1Department of Biological Sciences, Federal University Lokoja, Kogi State, Nigeria

2School of Biological Sciences, University of Aberdeen, Scotland, UK

*Corresponding Author: Awulu EA, Department of Biological Sciences, Federal University Lokoja, Kogi State, Nigeria and School of Biological Sciences, University of Aberdeen, Scotland, UK.

Received: May 22, 2023; Published: June 14, 2023

Abstract

The microbial SOS lux gene test was designed for quick screening of environmental mutagens. The assay is built on the receptor-reporter principle, with SOS as receptor sensitive to DNA damage and a bioluminescence system expressing detectable and measurable light signal. This bioassay was aimed at evaluating the effects of dimethyl sulfoxide (DMSO) on the bioluminescence expression of inducible SOS lux gene biosensor E. coli C600 pPSL-1 and E. coli DPD1718 elicited by Mitomycin C (a known mutagen). The bacterial culture of E. coli C600 pPSL-1 and E. coli DPD1718 at the mid-exponential growth phase were exposed to different concentrations of MMC (0, 10, 25, 75 and 100 ng/ml respectively) and with DMSO (0, 0.5, 1.0, 2.0 and 5.0 % respectively) for each MMC concentration). E. coli C600 pPSL-1 bioluminescence demonstrated a dose-dependent increase in SOS response that peaked at 3 hr post-MMC exposure, whereas E. coli DPD1718 bioluminescence demonstrated a gradual increase in bioluminescence which can be distinguished statistically same time. Above 1% DMSO concentrations significantly reduced the bioluminescence expression of E. coli C600 pPSL-1 and E. coli DPD1718. DMSO concentration above 1% caused significant reduction in bioluminescence in both microbial sensors. Hence, DMSO can cause additional toxicity in terms of hydrophobic compounds mobility across cell membrane of test subjects in toxicity evaluation and its role be screened meticulously.

 Keywords: Dimethyl Sulfoxide; Bioluminescence; Mitomycin C; E. coli C600 pPSL-1; E. coli DPD1718

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Citation

Citation: Awulu EA. “Effect of Dimethyl Sulfoxide on the Bioluminescence Expression of the Inducible SOS-Lux gene Biosensor E. coli C600 pPSL-1 and E. coli DPD1718 elicited by Mitomycin-C". Acta Scientific Microbiology 6.7 (2023): 22-26.

Copyright

Copyright: © 2023 Awulu EA. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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