Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 7

Synthesis of Oleoyl-diethanolamide by Using Purified Lipase from Thermotolerant Bacillus subtilis TTP-06

Manpreet Kaur and Reena Gupta*

Department of Biotechnology, Himachal Pradesh University, Shimla, Himachal Pradesh, India

*Corresponding Author: Reena Gupta, Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, Himachal Pradesh, India.

Received: May 08, 2023; Published: June 13, 2023

Abstract

Present study aimed to synthesize oleoyl-diethanolamide in a greener way by using lipase as a biocatalyst. The lipase enzyme used in present work was purified from a thermotolerant Bacillus subtilis TTP-06. The synthesis of oleoyl-diethanolamide was determined by checking the % conversion of oleic acid (OA). Therafter, oleoyl-diethanolamide synthesized in the laboratory was characterized by Fourier Transform Infra-Red (FTIR) spectroscopy. Maximum 92.64 ± 0.62% conversion of OA was achieved after the optimization of certain parameters viz. concentration of substrates, reaction time, reaction temperature and amount of biocatalyst.

 Keywords: Fourier Transform Infrared Spectroscopy; Oleoyl-Diethanolamide; Synthesis, Oleic Acid; Diethanolamine

References

  1. Masyithah Z and T Herawan. “Optimization of enzymatic synthesis of oleoyl-diethanolamide in solvent-free system”. Journal of Pure and Applied Microbiology 11 (2017): 1327-1336.
  2. Sood A., et al. “Lipases and their applications in biomedical field”. Current Biotechnology 12 (2023).
  3. Al-Mulla EAJ., et al. “Enzymatic synthesis of fatty amides from palm olein”. Journal of Oleo Science2 (2010): 59-64.
  4. Masyithah Z., et al. “The optimization of enzymatic synthesis for lauoryl-N-methyl glucamide surfactants”. Indonesian Journal of Chemistry 11 (2011): 223-228.
  5. Ayşenur A., et al. "Development of infusion tea formulations with food wastes: evaluation of temperature and time effects on quality parameters”. Applied Food Research1 (2022): 100087.
  6. Sánchez DA., et al. “Enzymatic synthesis of thymol octanoate, a promising hybrid molecule”. Catalysts3 (2023): 473.
  7. Suhendra D., et al. “Synthesis of fatty diethanolamide from calophyllum inophyllum kernel oil via enzymatic reaction”. Rasayan Journal of Chemistry 12.2 (2019): 765-772.
  8. Gunawan ER., et al. “Four factor surface response optimization of ethyl palmitate from Terminalia cattapa Kernel oil”. Asian Journal of Chemistry 29.10 (2017): 2107-2112.
  9. Bankar V., et al. “A practical green synthesis of thiazine derivatives using phase transfer catalyst”. Rasayan Journal of Chemistry 3 (2018): 1294-1299.
  10. Wang X., et al. “Synthesis of Oleoylethanolamide Using Lipase”. Journal of Agricultural and Food Chemistry1 (2012): 451-457.
  11. Masyithah Z. “Optimization method for the synthesis of fatty amide surfactants from oleic acid catalyzed by immobilized lipase”. Oriental Journal of Chemistry 32 (2016): 1361-1371.

Citation

Citation: Manpreet Kaur and Reena Gupta. “Synthesis of Oleoyl-diethanolamide by Using Purified Lipase from Thermotolerant Bacillus subtilis TTP-06". Acta Scientific Microbiology 6.7 (2023): 17-21.

Copyright

Copyright: © 2023 Manpreet Kaur and Reena Gupta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.




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