Acta Scientific Microbiology (ISSN: 2581-3226)

Research Article Volume 6 Issue 7

Synthesis of Oleoyl-diethanolamide by Using Purified Lipase from Thermotolerant Bacillus subtilis TTP-06

Manpreet Kaur and Reena Gupta*

Department of Biotechnology, Himachal Pradesh University, Shimla, Himachal Pradesh, India

*Corresponding Author: Reena Gupta, Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, Himachal Pradesh, India.

Received: May 08, 2023; Published: June 13, 2023


Present study aimed to synthesize oleoyl-diethanolamide in a greener way by using lipase as a biocatalyst. The lipase enzyme used in present work was purified from a thermotolerant Bacillus subtilis TTP-06. The synthesis of oleoyl-diethanolamide was determined by checking the % conversion of oleic acid (OA). Therafter, oleoyl-diethanolamide synthesized in the laboratory was characterized by Fourier Transform Infra-Red (FTIR) spectroscopy. Maximum 92.64 ± 0.62% conversion of OA was achieved after the optimization of certain parameters viz. concentration of substrates, reaction time, reaction temperature and amount of biocatalyst.

 Keywords: Fourier Transform Infrared Spectroscopy; Oleoyl-Diethanolamide; Synthesis, Oleic Acid; Diethanolamine


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Citation: Manpreet Kaur and Reena Gupta. “Synthesis of Oleoyl-diethanolamide by Using Purified Lipase from Thermotolerant Bacillus subtilis TTP-06". Acta Scientific Microbiology 6.7 (2023): 17-21.


Copyright: © 2023 Manpreet Kaur and Reena Gupta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.


Acceptance rate30%
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