Manpreet Kaur and Reena Gupta*
Department of Biotechnology, Himachal Pradesh University, Shimla, Himachal Pradesh, India
*Corresponding Author: Reena Gupta, Department of Biotechnology, Himachal Pradesh University, Summerhill, Shimla, Himachal Pradesh, India.
Received: May 08, 2023; Published: June 13, 2023
Present study aimed to synthesize oleoyl-diethanolamide in a greener way by using lipase as a biocatalyst. The lipase enzyme used in present work was purified from a thermotolerant Bacillus subtilis TTP-06. The synthesis of oleoyl-diethanolamide was determined by checking the % conversion of oleic acid (OA). Therafter, oleoyl-diethanolamide synthesized in the laboratory was characterized by Fourier Transform Infra-Red (FTIR) spectroscopy. Maximum 92.64 ± 0.62% conversion of OA was achieved after the optimization of certain parameters viz. concentration of substrates, reaction time, reaction temperature and amount of biocatalyst.
Keywords: Fourier Transform Infrared Spectroscopy; Oleoyl-Diethanolamide; Synthesis, Oleic Acid; Diethanolamine
Citation: Manpreet Kaur and Reena Gupta. “Synthesis of Oleoyl-diethanolamide by Using Purified Lipase from Thermotolerant Bacillus subtilis TTP-06". Acta Scientific Microbiology 6.7 (2023): 17-21.
Copyright: © 2023 Manpreet Kaur and Reena Gupta. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.